PMID- 36185682
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221004
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 9
DP  - 2022
TI  - Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer 
      arietinum L.).
PG  - 966705
LID - 10.3389/fnut.2022.966705 [doi]
LID - 966705
AB  - Chickpea (Cicer arietinum L.) is a well-known legume widely used as traditional 
      medicine. This study aimed to characterize the structure and evaluate the 
      immunomodulatory activity of one glycoprotein [crude chickpea glycoprotein-1 
      (CAG-1)] isolated from chickpea. CAG-1 was extracted with hot alkaline water and 
      purified with DEAE-Sepharose Fast Flow and Superdex-200 column chromatography. 
      CAG-1, with a molecular weight of 8,106 Da, contained 57.12% polysaccharide and 
      35.41% protein. The polysaccharide part was mainly composed of glucose (Glc). The 
      protein part was connected mainly by aspartic (Asp) and glutamic (Glu). The 
      results of nuclear magnetic resonance (NMR) analysis indicated the presence of 
      alpha-d-Glcp-(1 --> 4)-alpha-d-Glcp-(1 --> 4)-alpha-d-Glcp-(1 --> . In addition, the sugar chains 
      of the glycoprotein were not hydrolyzed under alkaline conditions, suggesting 
      that the glycoprotein was N-glycosidic; thus, the sugar chain was linked to the 
      protein chain by Asp. An immunological study showed that CAG-1 stimulated the 
      production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-alpha 
      (TNF-alpha), and monocyte chemotactic protein 1 (MCP-1) in RAW 264.7 macrophages in a 
      dose-dependent manner.
CI  - Copyright (c) 2022 Shi, Li, Wei, Wang, Zhou, Ma and Yao.
FAU - Shi, Zhenxing
AU  - Shi Z
AD  - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 
      China.
AD  - Department of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing, 
      China.
AD  - School of Food Science and Technology, Henan University of Technology, Zhengzhou, 
      China.
FAU - Li, Shiyu
AU  - Li S
AD  - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 
      China.
FAU - Wei, Zuchen
AU  - Wei Z
AD  - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 
      China.
AD  - Laboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir 
      Area's Medicinal Herbs, College of Life Science and Engineering, Chongqing Three 
      Gorges University, Chongqing, China.
FAU - Wang, Yuanji
AU  - Wang Y
AD  - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 
      China.
FAU - Zhou, Nong
AU  - Zhou N
AD  - Laboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir 
      Area's Medicinal Herbs, College of Life Science and Engineering, Chongqing Three 
      Gorges University, Chongqing, China.
FAU - Ma, Qiang
AU  - Ma Q
AD  - Department of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing, 
      China.
FAU - Yao, Yang
AU  - Yao Y
AD  - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220916
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC9523481
OTO - NOTNLM
OT  - RAW 264.7 murine macrophage cell
OT  - chickpea
OT  - glycoprotein
OT  - immunomodulatory
OT  - structural characterization
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/10/04 06:00
MHDA- 2022/10/04 06:01
PMCR- 2022/01/01
CRDT- 2022/10/03 04:23
PHST- 2022/06/11 00:00 [received]
PHST- 2022/07/15 00:00 [accepted]
PHST- 2022/10/03 04:23 [entrez]
PHST- 2022/10/04 06:00 [pubmed]
PHST- 2022/10/04 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2022.966705 [doi]
PST - epublish
SO  - Front Nutr. 2022 Sep 16;9:966705. doi: 10.3389/fnut.2022.966705. eCollection 
      2022.