PMID- 36185700
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221004
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 9
DP  - 2022
TI  - Purification, structural characterization and immunological activity of Sibiraea 
      laexigata (L.) Maxim polysaccharide.
PG  - 1013020
LID - 10.3389/fnut.2022.1013020 [doi]
LID - 1013020
AB  - Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating 
      stomach discomfort and indigestion for a long time in china. Polysaccharides have 
      been identified as one of the major bioactive compounds in the SLM. In the 
      present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was 
      employed in polysaccharides extraction derived from SLM using polyethylene glycol 
      (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 
      and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 
      chromatography in sequence. Then, the preliminarily structure of the two factions 
      were characterized by chemical composition analysis, molecular weight 
      measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra 
      analysis and SEM. The results showed that SLMPs-1-1 and SLMPs-2-1 with different 
      molecular weights of 1.03 and 1.02 kDa, mainly composed of glucose (46.76 and 
      46.79%), respectively. The results of structural characterization from FT-IR, 
      (1)H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical 
      pyranoid polysaccharide with alpha-glycosidic bond and beta-glycosidic bond. 
      Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor 
      necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2), and alleviated the immune 
      organs tissue damage of cyclophosphamide (Cy)-treated mice. RT-qPCR and 
      Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the 
      levels of NF-kappaB, TLR4, which revealed that SLMPs-1-1 could participate in 
      immunosuppressive protection of Cy-treated mice. These findings suggested that 
      the potential of SLMPs-1-1 as an alternative immunostimulator could be used in 
      food and pharmaceutical industries.
CI  - Copyright (c) 2022 Yang, Liu, Yang, Ma, Guo, Chen and Gao.
FAU - Yang, Xuhua
AU  - Yang X
AD  - China-Malaysia National Joint Laboratory, College of Life Sciences and 
      Engineering, Northwest Minzu University, Lanzhou, China.
FAU - Liu, Honghai
AU  - Liu H
AD  - Technology Research and Development Center, Gansu Tobacco Industry Co., Ltd., 
      Lanzhou, China.
FAU - Yang, Jutian
AU  - Yang J
AD  - China-Malaysia National Joint Laboratory, College of Life Sciences and 
      Engineering, Northwest Minzu University, Lanzhou, China.
AD  - Taizishan Ecosystem Observatory of Carbon Neutralization, Northwest Minzu 
      University, Lanzhou, China.
FAU - Ma, Zhongren
AU  - Ma Z
AD  - China-Malaysia National Joint Laboratory, College of Life Sciences and 
      Engineering, Northwest Minzu University, Lanzhou, China.
FAU - Guo, Penghui
AU  - Guo P
AD  - China-Malaysia National Joint Laboratory, College of Life Sciences and 
      Engineering, Northwest Minzu University, Lanzhou, China.
AD  - Taizishan Ecosystem Observatory of Carbon Neutralization, Northwest Minzu 
      University, Lanzhou, China.
FAU - Chen, Hong
AU  - Chen H
AD  - China-Malaysia National Joint Laboratory, College of Life Sciences and 
      Engineering, Northwest Minzu University, Lanzhou, China.
FAU - Gao, Dandan
AU  - Gao D
AD  - China-Malaysia National Joint Laboratory, College of Life Sciences and 
      Engineering, Northwest Minzu University, Lanzhou, China.
AD  - Taizishan Ecosystem Observatory of Carbon Neutralization, Northwest Minzu 
      University, Lanzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220915
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC9521201
OTO - NOTNLM
OT  - Sibiraea laexigata (L.) Maxim
OT  - immunological activity
OT  - polysaccharides
OT  - purification
OT  - structural characterization
COIS- Author HL was employed by the Gansu Tobacco Industry Co., Ltd. The remaining 
      authors declare that the research was conducted in the absence of any commercial 
      or financial relationships that could be construed as a potential conflict of 
      interest.
EDAT- 2022/10/04 06:00
MHDA- 2022/10/04 06:01
PMCR- 2022/01/01
CRDT- 2022/10/03 04:23
PHST- 2022/08/06 00:00 [received]
PHST- 2022/08/29 00:00 [accepted]
PHST- 2022/10/03 04:23 [entrez]
PHST- 2022/10/04 06:00 [pubmed]
PHST- 2022/10/04 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2022.1013020 [doi]
PST - epublish
SO  - Front Nutr. 2022 Sep 15;9:1013020. doi: 10.3389/fnut.2022.1013020. eCollection 
      2022.