PMID- 36187791
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221004
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 13
DP  - 2022
TI  - LncRNA affects epigenetic reprogramming of porcine embryo development by 
      regulating global epigenetic modification and the downstream gene SIN3A.
PG  - 971965
LID - 10.3389/fphys.2022.971965 [doi]
LID - 971965
AB  - The study of preimplantation development is of great significance to reproductive 
      biology and regenerative medicine. With the development of high-throughput deep 
      sequencing technology, it has been found that lncRNAs play a very important role 
      in the regulation of embryonic development. In this study, key lncRNAs that 
      regulate embryonic development were screened by analyzing the expression pattern 
      of lncRNAs in porcine in vivo fertilization (IVV) embryos. By knocking down 
      lncRNA expression in in vitro fertilization (IVF) embryos, we investigated its 
      function and mechanism of regulating embryonic development. The results showed 
      that the expression pattern of lncRNA was consistent with the time of gene 
      activation. The lncRNAs were highly expressed in the 4-cell to blastocyst stage 
      but barely expressed in the oocytes and 2-cell stage. So we speculated this part 
      of lncRNAs may regulate gene expression. The lncRNA LOC102165808 (named lncT 
      because the gene near this lncRNA is TFAP2C) was one of them. The knockdown (KD) 
      of lncT inhibited embryonic development, resulting in decreased H3K4me3, H3K4me2, 
      and H3K9me3, and increased DNA methylation. Meanwhile, RNAseq showed SIN3A was 
      the top decreased gene in lncT-KD embryos. There was a severe blastocyst 
      formation defect in SIN3A-KD embryos. Both lncT and SIN3A could affect NANOG and 
      induce more cell apoptosis. In conclusion, the knockdown of lncT inhibits 
      embryonic development by regulating H3K4me3, H3K4me2, DNA methylation, 
      pluripotency gene, and apoptosis, and SIN3A is one of the downstream genes of 
      lncT in regulating embryonic development.
CI  - Copyright (c) 2022 Zhang, Zhou, Huang, Zhai, Wu, An, Zhang, Shi, Li, Kong, Yu and 
      Li.
FAU - Zhang, Daoyu
AU  - Zhang D
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Zhou, Yongfeng
AU  - Zhou Y
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Huang, Rong
AU  - Huang R
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Zhai, Yanhui
AU  - Zhai Y
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Wu, Di
AU  - Wu D
AD  - Department of Emergency Medicine, First Hospital, Jilin University, Changchun, 
      China.
FAU - An, Xinglan
AU  - An X
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Zhang, Sheng
AU  - Zhang S
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Shi, Lijing
AU  - Shi L
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Li, Qi
AU  - Li Q
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Kong, Xiangjie
AU  - Kong X
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
FAU - Yu, Hao
AU  - Yu H
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Li, Ziyi
AU  - Li Z
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, First Hospital, Jilin University, Changchun, China.
LA  - eng
PT  - Journal Article
DEP - 20220916
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC9523245
OTO - NOTNLM
OT  - DNA methylation
OT  - SIN3A
OT  - histone modification
OT  - lncRNAs
OT  - porcine embryonic development
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/10/04 06:00
MHDA- 2022/10/04 06:01
PMCR- 2022/09/16
CRDT- 2022/10/03 04:58
PHST- 2022/06/17 00:00 [received]
PHST- 2022/08/30 00:00 [accepted]
PHST- 2022/10/03 04:58 [entrez]
PHST- 2022/10/04 06:00 [pubmed]
PHST- 2022/10/04 06:01 [medline]
PHST- 2022/09/16 00:00 [pmc-release]
AID - 971965 [pii]
AID - 10.3389/fphys.2022.971965 [doi]
PST - epublish
SO  - Front Physiol. 2022 Sep 16;13:971965. doi: 10.3389/fphys.2022.971965. eCollection 
      2022.