PMID- 36188117
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221004
IS  - 1948-0210 (Print)
IS  - 1948-0210 (Electronic)
IS  - 1948-0210 (Linking)
VI  - 14
IP  - 9
DP  - 2022 Sep 26
TI  - Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces 
      differentiation of mesenchymal stem cells into cardiac-like cells.
PG  - 700-713
LID - 10.4252/wjsc.v14.i9.700 [doi]
AB  - BACKGROUND: Heart diseases are the primary cause of death all over the world. 
      Following myocardial infarction, billions of cells die, resulting in a huge loss 
      of cardiac function. Stem cell-based therapies have appeared as a new area to 
      support heart regeneration. The transcription factors GATA binding protein 4 
      (GATA-4) and myocyte enhancer factor 2C (MEF2C) are considered prominent factors 
      in the development of the cardiovascular system. AIM: To explore the potential of 
      GATA-4 and MEF2C for the cardiac differentiation of human umbilical cord 
      mesenchymal stem cells (hUC-MSCs). METHODS: hUC-MSCs were characterized 
      morphologically and immunologically by the presence of specific markers of MSCs 
      via immunocytochemistry and flow cytometry, and by their potential to 
      differentiate into osteocytes and adipocytes. hUC-MSCs were transfected with 
      GATA-4, MEF2C, and their combination to direct the differentiation. Cardiac 
      differentiation was confirmed by semiquantitative real-time polymerase chain 
      reaction and immunocytochemistry. RESULTS: hUC-MSCs expressed specific cell 
      surface markers CD105, CD90, CD44, and vimentin but lack the expression of CD45. 
      The transcription factors GATA-4 and MEF2C, and their combination induced 
      differentiation in hUC-MSCs with significant expression of cardiac genes i.e., 
      GATA-4, MEF2C, NK2 homeobox 5 (NKX2.5), MHC, and connexin-43, and cardiac 
      proteins GATA-4, NKX2.5, cardiac troponin T, and connexin-43. CONCLUSION: 
      Transfection with GATA-4, MEF2C, and their combination effectively induces 
      cardiac differentiation in hUC-MSCs. These genetically modified MSCs could be a 
      promising treatment option for heart diseases in the future.
CI  - (c)The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights 
      reserved.
FAU - Razzaq, Syeda Saima
AU  - Razzaq SS
AD  - Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi 75270, 
      Pakistan.
FAU - Khan, Irfan
AU  - Khan I
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi 
      75270, Pakistan.
FAU - Naeem, Nadia
AU  - Naeem N
AD  - Dow Research Institute of Biotechnology & Biomedical Sciences (DRIBBS), Dow 
      University of Health Sciences (DUHS), Ojha Campus, Karachi 75200, Pakistan.
FAU - Salim, Asmat
AU  - Salim A
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi 
      75270, Pakistan.
FAU - Begum, Sumreen
AU  - Begum S
AD  - Stem Cells Research Laboratory (SCRL), Sindh Institute of Urology and 
      Transplantation (SIUT), Karachi 74200, Pakistan.
FAU - Haneef, Kanwal
AU  - Haneef K
AD  - Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi 75270, 
      Pakistan. k.haneef@uok.edu.pk.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Stem Cells
JT  - World journal of stem cells
JID - 101535826
PMC - PMC9516467
OTO - NOTNLM
OT  - Differentiation
OT  - GATA binding protein 4
OT  - Heart disease
OT  - Human umbilical cord-mesenchymal stem cells
OT  - Myocyte enhancer factor 2C
OT  - Transcription factors
COIS- Conflict-of-interest statement: The authors confirm that this article has no 
      conflict of interest to report.
EDAT- 2022/10/04 06:00
MHDA- 2022/10/04 06:01
PMCR- 2022/09/26
CRDT- 2022/10/03 05:03
PHST- 2022/03/28 00:00 [received]
PHST- 2022/06/20 00:00 [revised]
PHST- 2022/08/30 00:00 [accepted]
PHST- 2022/10/03 05:03 [entrez]
PHST- 2022/10/04 06:00 [pubmed]
PHST- 2022/10/04 06:01 [medline]
PHST- 2022/09/26 00:00 [pmc-release]
AID - 10.4252/wjsc.v14.i9.700 [doi]
PST - ppublish
SO  - World J Stem Cells. 2022 Sep 26;14(9):700-713. doi: 10.4252/wjsc.v14.i9.700.