PMID- 36191853
OWN - NLM
STAT- MEDLINE
DCOM- 20221108
LR  - 20221213
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Linking)
VI  - 150
DP  - 2022 Nov
TI  - Gonadulin: A newly discovered insulin-like peptide involved in ovulation and 
      oviposition in Rhodnius prolixus, a vector of Chagas disease.
PG  - 103848
LID - S0965-1748(22)00130-8 [pii]
LID - 10.1016/j.ibmb.2022.103848 [doi]
AB  - Insulin-like peptides (ILPs) are vital hormones involved in a wide range of 
      physiological processes in all organisms. In insects, insulin signaling has a key 
      role in detecting and interpreting nutrient levels for egg production. Based on 
      publicly available transcriptomes, a new ILP named gonadulin has been reported 
      and suggested to be expressed by the gonads (hence its name). Although the 
      identification of gonadulin establishes its existence, its physiological 
      relevance remains poorly understood. Rhodnius prolixus is an obligate 
      hematophagous insect and a primary vector of Trypanosoma cruzi, the etiological 
      agent of Chagas disease. In this study, we report for the first time the 
      participation of gonadulin in reproductive performance of an hemipteran. By 
      quantitative PCR and fluorescence in situ hybridization (FISH), we find that the 
      R. prolixus gonadulin transcript is highly expressed in the reproductive system, 
      particularly in the calyx, a structure through which eggs move into the lumen of 
      the lateral oviducts during ovulation. The putative gonadulin receptor, a member 
      of the leucine-rich repeat-containing G protein-coupled receptor subfamily 
      (LGR3), is most highly expressed in the central nervous system with lower levels 
      in the reproductive tissue and other tissues. Interestingly, when the gonadulin 
      signaling cascade is impaired using RNA interference (RNAi), eggs are retained 
      primarily in the ovarioles and calyx, indicating that ovulation and oviposition 
      are inhibited. Understanding the physiological processes involved in reproduction 
      in R. prolixus will shed light on potential targets for effective production of 
      biopesticides by translational research, thereby controlling insect populations 
      and transmission of the disease.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Leyria, Jimena
AU  - Leyria J
AD  - Department of Biology, University of Toronto Mississauga, Mississauga, ON, 
      Canada. Electronic address: jimenal.leyria@utoronto.ca.
FAU - Philip, Riya
AU  - Philip R
AD  - Department of Biology, University of Toronto Mississauga, Mississauga, ON, 
      Canada. Electronic address: riya.philip@mail.utoronto.ca.
FAU - Orchard, Ian
AU  - Orchard I
AD  - Department of Biology, University of Toronto Mississauga, Mississauga, ON, 
      Canada. Electronic address: ian.orchard@utoronto.ca.
FAU - Lange, Angela B
AU  - Lange AB
AD  - Department of Biology, University of Toronto Mississauga, Mississauga, ON, 
      Canada. Electronic address: angela.lange@utoronto.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220930
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Insulin)
RN  - 0 (Peptides)
SB  - IM
MH  - Female
MH  - Animals
MH  - *Rhodnius/genetics
MH  - Oviposition
MH  - Insulin/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Chagas Disease
MH  - Peptides/genetics
MH  - Ovulation
OTO - NOTNLM
OT  - GPCR
OT  - Insect
OT  - Insulin
OT  - RNAi
OT  - Reproduction
OT  - Reproductive system
COIS- Declaration of competing interest The authors declare no competing interests.
EDAT- 2022/10/04 06:00
MHDA- 2022/11/09 06:00
CRDT- 2022/10/03 19:28
PHST- 2022/08/27 00:00 [received]
PHST- 2022/09/27 00:00 [revised]
PHST- 2022/09/27 00:00 [accepted]
PHST- 2022/10/04 06:00 [pubmed]
PHST- 2022/11/09 06:00 [medline]
PHST- 2022/10/03 19:28 [entrez]
AID - S0965-1748(22)00130-8 [pii]
AID - 10.1016/j.ibmb.2022.103848 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2022 Nov;150:103848. doi: 10.1016/j.ibmb.2022.103848. 
      Epub 2022 Sep 30.