PMID- 36193063
OWN - NLM
STAT- MEDLINE
DCOM- 20221005
LR  - 20240118
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - Tumor Necrosis Factor-Alpha Disrupts Cx43-Mediated Corneal Endothelial Gap 
      Junction Intercellular Communication.
PG  - 4824699
LID - 10.1155/2022/4824699 [doi]
LID - 4824699
AB  - Connexin43 (Cx43)-mediated gap junctions are vital in maintaining corneal 
      endothelium homeostasis. Tumor necrosis factor-alpha (TNF-alpha) is among the most 
      important inflammatory factors which cause corneal endothelial dysfunction in 
      various eye diseases. However, the effect of TNF-alpha on Cx43-mediated gap junctions 
      of the corneal endothelium remains undefined. In the current research, we 
      determined the effect of TNF-alpha on gap junction intercellular communication (GJIC) 
      in rabbit corneal endothelium. To evaluate alterations of GJIC, if any, we 
      treated ex vivo cultured rabbit corneal endothelium with different concentrations 
      of TNF-alpha (2-20 ng/ml). The localization of Cx43 was analyzed by immunostaining, 
      while RT-qPCR and western blot were used to profile the expression of Cx43 and 
      zonula occludens-1 (ZO-1). The association between ZO-1 and Cx43 was evaluated 
      using immunoprecipitation and double staining. GJIC activity was determined by 
      the scrap loading and dye transfer assay (SLDT). Our data demonstrated that a 
      high concentration of TNF-alpha (10 ng/ml and 20 ng/ml) disrupts the Cx43 mediated 
      gap junction distribution in rabbit corneal endothelium and suppresses the 
      expression of Cx43 protein. Furthermore, rabbit corneal endothelial GJIC was 
      inhibited due to the decreased association between the ZO-1 and Cx43 proteins. 
      Current results demonstrate that TNF-alpha inhibits corneal endothelial GJIC via 
      decreasing the association between ZO-1 and Cx43, disrupting the distribution of 
      Cx43, and downregulating the expression of Cx43 protein. This study offers a new 
      theoretical foundation for diagnosing and treating corneal endothelial cell 
      decompensation induced by elevated TNF-alpha in various eye diseases.
CI  - Copyright (c) 2022 Jufeng Meng et al.
FAU - Meng, Jufeng
AU  - Meng J
AUID- ORCID: 0000-0002-3035-7225
AD  - School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, 
      China.
FAU - Xu, Ke
AU  - Xu K
AUID- ORCID: 0000-0002-4423-1703
AD  - Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC 
      Key Laboratory of Control of Tropical Diseases, School of Tropical Medicine, 
      Hainan Medical University, Haikou 571199, China.
FAU - Qin, Yinyin
AU  - Qin Y
AD  - Institute of Clinical Medicine Research, Suzhou Science & Technology Town 
      Hospital, Suzhou 215153, China.
FAU - Liu, Ya
AU  - Liu Y
AD  - Department of Ophthalmology, Suzhou Science & Technology Town Hospital, Suzhou 
      215153, China.
FAU - Xu, Lin
AU  - Xu L
AD  - Department of Ophthalmology, Suzhou Science & Technology Town Hospital, Suzhou 
      215153, China.
FAU - Qiao, Shigang
AU  - Qiao S
AUID- ORCID: 0000-0003-0309-8405
AD  - Institute of Clinical Medicine Research, Suzhou Science & Technology Town 
      Hospital, Suzhou 215153, China.
FAU - An, Jianzhong
AU  - An J
AD  - Institute of Clinical Medicine Research, Suzhou Science & Technology Town 
      Hospital, Suzhou 215153, China.
FAU - Liu, Jianjun
AU  - Liu J
AD  - Department of Ophthalmology, Suzhou Science & Technology Town Hospital, Suzhou 
      215153, China.
FAU - Zhang, Zhenhao
AU  - Zhang Z
AUID- ORCID: 0000-0001-5659-6315
AD  - Institute of Clinical Medicine Research, Suzhou Science & Technology Town 
      Hospital, Suzhou 215153, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20220919
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Connexin 43)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
RIN - Oxid Med Cell Longev. 2024 Jan 9;2024:9871986. PMID: 38234513
MH  - Animals
MH  - Cell Communication/physiology
MH  - Cells, Cultured
MH  - *Connexin 43
MH  - Gap Junctions/metabolism
MH  - Rabbits
MH  - *Tumor Necrosis Factor-alpha/metabolism
PMC - PMC9526630
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/10/05 06:00
MHDA- 2022/10/06 06:00
PMCR- 2022/09/19
CRDT- 2022/10/04 01:54
PHST- 2022/07/10 00:00 [received]
PHST- 2022/08/23 00:00 [revised]
PHST- 2022/08/27 00:00 [accepted]
PHST- 2022/10/04 01:54 [entrez]
PHST- 2022/10/05 06:00 [pubmed]
PHST- 2022/10/06 06:00 [medline]
PHST- 2022/09/19 00:00 [pmc-release]
AID - 10.1155/2022/4824699 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 Sep 19;2022:4824699. doi: 10.1155/2022/4824699. 
      eCollection 2022.