PMID- 36194269 OWN - NLM STAT- MEDLINE DCOM- 20230224 LR - 20230224 IS - 1436-6215 (Electronic) IS - 1436-6207 (Print) IS - 1436-6207 (Linking) VI - 62 IP - 2 DP - 2023 Mar TI - Oral administration of Lactobacillus plantarum JC7 alleviates OVA-induced murine food allergy through immunoregulation and restoring disordered intestinal microbiota. PG - 685-698 LID - 10.1007/s00394-022-03016-5 [doi] AB - PURPOSE: The incidence and prevalence of food allergy have sharply risen over the past several decades. Oral administration of probiotic stains has been proven as a safe and effective method to control food allergy. In this study, it aims to comprehensively investigate the anti-allergic effect of Lactobacillus plantarum JC7. METHODS: Balb/c mice were randomly divided into three groups and received OVA (20 microg/mouse, intraperitoneal injection), L. plantarum JC7 (2 x 10(8) CFU/mouse, intragastric administration) + OVA (20 microg/mouse, intraperitoneal injection) or 0.9% saline (intragastric administration) for 3 weeks. Body weight was monitored weekly, and allergic reactions were evaluated after challenge of OVA. Serum levels of OVA-specific immunoglobulins and various cytokines were tested using ELISA, and the cecum microbiota was analysed by 16S rRNA sequencing to explore the relationships between these indicators and OVA-induced food allergy. Western blotting was used to identify the expression levels of phosphorylated IkappaBalpha and nuclear factor kappa B p65. RESULTS: OVA-sensitised mice showed mitigation of respiratory manifestations, alleviation of lung inflammation and congestion, and the presence of an intact intestinal villus structure. Furthermore, OVA-specific immunoglobulin E (IgE), OVA-specific-IgG1, and plasma histamine levels were declined in mice treated with L. plantarum JC7 than in OVA-sensitised mice. In addition, interferon-gamma (IFN-gamma) and interleukin 10 (IL-10) levels were significantly increased, while IL-4 and IL-17A levels were clearly decreased in mice that had undergone oral administration of L. plantarum JC7, compared with OVA-sensitised mice. These findings indicated imbalances of T helper cell type 1 (Th1)/Th2 and regulatory T cells (Treg)/Th17, which were confirmed by quantitative polymerase chain reaction (PCR). Western blotting demonstrated that the expression levels of phosphorylated IkappaBalpha and nuclear factor kappa B p65 were significantly increased in OVA-sensitised mice, but these changes were partly reversed after treatment with L. plantarum JC7. Oral administration of L. plantarum JC7 increased the richness, diversity, and evenness of cecum microbiota, characterised by higher Bacteroidetes abundance and lower Firmicutes abundance. Additionally, the intestinal microbial community composition was significantly altered in the OVA-sensitised group, indicating a disordered intestinal microbiota that was restored by the oral administration of L. plantarum JC7. CONCLUSION: Overall, L. plantarum JC7 can prevent food allergy by rectifying Th1/Th2 and Treg/Th17 imbalances, combined with modifications of disordered intestinal microbiota. CI - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany. FAU - Duan, Cuicui AU - Duan C AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Ma, Lin AU - Ma L AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Yu, Jie AU - Yu J AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Sun, Yixue AU - Sun Y AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Liu, Lifan AU - Liu L AD - Graduate School, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Ma, Fumin AU - Ma F AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Li, Xiaolei AU - Li X AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. FAU - Li, Dan AU - Li D AUID- ORCID: 0000-0002-5711-9665 AD - Key Laboratory of Agro-Products Processing Technology, Jilin Provincial Department of Education, Changchun University, 6543 Weixing Road, Changchun, 130022, Jilin, People's Republic of China. drlidan@yahoo.com. LA - eng GR - YDZJ202201ZYTS688/Department of Science and Technology, Jilin Province/ GR - ZKP202110/the Scholar Climbing Project of Changchun University/ PT - Journal Article DEP - 20221004 PL - Germany TA - Eur J Nutr JT - European journal of nutrition JID - 100888704 RN - 9006-59-1 (Ovalbumin) RN - 139874-52-5 (NF-KappaB Inhibitor alpha) RN - 0 (NF-kappa B) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Cytokines) SB - IM MH - Mice MH - Animals MH - *Lactobacillus plantarum/genetics/metabolism MH - Ovalbumin MH - NF-KappaB Inhibitor alpha/therapeutic use MH - *Gastrointestinal Microbiome MH - NF-kappa B MH - RNA, Ribosomal, 16S MH - *Food Hypersensitivity/drug therapy MH - Cytokines/metabolism MH - Administration, Oral MH - Mice, Inbred BALB C MH - Disease Models, Animal PMC - PMC9530419 OTO - NOTNLM OT - Food allergy OT - Gut microbiota OT - Lactobacillus plantarum OT - Th1/Th2 imbalance OT - Treg/Th17 imbalance COIS- The authors declare no completing financial interest or personal relationship that could have appeared to influence the work reported in this paper. EDAT- 2022/10/05 06:00 MHDA- 2023/02/25 06:00 PMCR- 2022/10/04 CRDT- 2022/10/04 11:13 PHST- 2022/04/05 00:00 [received] PHST- 2022/09/22 00:00 [accepted] PHST- 2022/10/05 06:00 [pubmed] PHST- 2023/02/25 06:00 [medline] PHST- 2022/10/04 11:13 [entrez] PHST- 2022/10/04 00:00 [pmc-release] AID - 10.1007/s00394-022-03016-5 [pii] AID - 3016 [pii] AID - 10.1007/s00394-022-03016-5 [doi] PST - ppublish SO - Eur J Nutr. 2023 Mar;62(2):685-698. doi: 10.1007/s00394-022-03016-5. Epub 2022 Oct 4.