PMID- 36194390
OWN - NLM
STAT- MEDLINE
DCOM- 20221027
LR  - 20221222
IS  - 1530-6992 (Electronic)
IS  - 1530-6984 (Linking)
VI  - 22
IP  - 20
DP  - 2022 Oct 26
TI  - Phosphatidylserine Lipid Nanoparticles Promote Systemic RNA Delivery to Secondary 
      Lymphoid Organs.
PG  - 8304-8311
LID - 10.1021/acs.nanolett.2c03234 [doi]
AB  - Secondary lymphoid organs (SLOs) are an important target for mRNA delivery in 
      various applications. While the current delivery method relies on the drainage of 
      nanoparticles to lymph nodes by intramuscular (IM) or subcutaneous (SC) 
      injections, an efficient mRNA delivery carrier for SLOs-targeting delivery by 
      systemic administration (IV) is highly desirable but yet to be available. In this 
      study, we developed an efficient SLOs-targeting carrier using phosphatidylserine 
      (PS), a well-known signaling molecule that promotes the endocytic activity of 
      phagocytes and cellular entry of enveloped viruses. We adopted these biomimetic 
      strategies and added PS into the standard four-component MC3-based LNP 
      formulation (PS-LNP) to facilitate the cellular uptake of immune cells beyond the 
      charge-driven targeting principle commonly used today. As a result, PS-LNP 
      performed efficient protein expression in both lymph nodes and the spleen after 
      IV administration. In vitro and in vivo characterizations on PS-LNP demonstrated 
      a monocyte/macrophage-mediated SLOs-targeting delivery mechanism.
FAU - Luozhong, Sijin
AU  - Luozhong S
AUID- ORCID: 0000-0001-8697-9480
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
FAU - Yuan, Zhefan
AU  - Yuan Z
AUID- ORCID: 0000-0002-4724-6214
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
FAU - Sarmiento, Tara
AU  - Sarmiento T
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
FAU - Chen, Yu
AU  - Chen Y
AD  - Department of Materials Science and Engineering, Cornell University, Ithaca, New 
      York 14853, United States.
FAU - Gu, Wenchao
AU  - Gu W
AUID- ORCID: 0000-0002-0967-1682
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
FAU - McCurdy, Caleb
AU  - McCurdy C
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
FAU - Gao, Wenting
AU  - Gao W
AD  - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 
      14853, United States.
FAU - Li, Ruoxin
AU  - Li R
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
FAU - Wilkens, Stephan
AU  - Wilkens S
AD  - Department of Biochemistry and Molecular Biology, SUNY Upstate Medical 
      University, Syracuse, New York 13210, United States.
FAU - Jiang, Shaoyi
AU  - Jiang S
AUID- ORCID: 0000-0001-9863-6899
AD  - Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York 
      14853, United States.
LA  - eng
GR  - S10 OD025049/OD/NIH HHS/United States
GR  - R35 GM141908/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20221004
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
RN  - 0 (Lipid Nanoparticles)
RN  - 0 (Phosphatidylserines)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Phosphatidylserines
MH  - *Nanoparticles/chemistry
MH  - RNA, Small Interfering/genetics
MH  - RNA, Messenger/genetics
OTO - NOTNLM
OT  - cell-targeting delivery
OT  - lipid nanoparticles
OT  - mRNA delivery
OT  - secondary lymphoid organs
EDAT- 2022/10/05 06:00
MHDA- 2022/10/28 06:00
CRDT- 2022/10/04 11:22
PHST- 2022/10/05 06:00 [pubmed]
PHST- 2022/10/28 06:00 [medline]
PHST- 2022/10/04 11:22 [entrez]
AID - 10.1021/acs.nanolett.2c03234 [doi]
PST - ppublish
SO  - Nano Lett. 2022 Oct 26;22(20):8304-8311. doi: 10.1021/acs.nanolett.2c03234. Epub 
      2022 Oct 4.