PMID- 36194447
OWN - NLM
STAT- MEDLINE
DCOM- 20221104
LR  - 20221104
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 39
IP  - 11
DP  - 2022 Nov
TI  - Validation of a LC-MS/MS method for the quantitative analysis of four antibiotics 
      in pig tissues and plasma to assess the risk of transfer of residues to edible 
      matrices after exposure to cross-contaminated feed.
PG  - 1818-1827
LID - 10.1080/19440049.2022.2126529 [doi]
AB  - Cross-contamination between medicated and non-medicated feed can occur during 
      production, processing, transport or storage of animal feed. This may lead to the 
      presence of low concentrations of antibiotics in supposedly drug-free feed for 
      food production animals, which potentially could also harm consumers due to 
      residues. In addition, consumption of sub-therapeutic concentrations of 
      antibiotics may increase the risk of emergence of resistant bacteria. In this 
      study, LC-MS/MS methods were developed to quantify four antibiotics 
      (sulfadimethoxine, oxytetracycline, trimethoprim and amoxicillin) in several pig 
      matrices, i.e. plasma, muscle, liver, kidneys and faeces. All methods were 
      validated using the accuracy profile, except for amoxicillin in faeces, for which 
      extraction could not be optimised for low concentrations. These methods were then 
      applied as part of an animal study during which several pigs received 
      contaminated feed at a concentration corresponding to 2% of therapeutic dose, in 
      order to evaluate the risk of the presence of residues in animal faeces and 
      tissues. The results showed that sulfadimethoxine is well absorbed and 
      accumulates in the muscle, kidneys and liver, where concentrations were higher 
      than the maximum residue limits (MRLs) authorised in EU legislation. Conversely, 
      oxytetracycline was mostly found in faeces as its oral absorption is very low. 
      Trimethoprim concentrations were slightly higher than the tolerated MRL in the 
      kidneys, but they were below this level in the other tissues. Finally, 
      amoxicillin concentrations remained below the lower limit of quantification of 
      the methods in all matrices.
FAU - Santos-Santorum Suarez, Cristina
AU  - Santos-Santorum Suarez C
AUID- ORCID: 0000-0001-9723-0336
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Sanders, Pascal
AU  - Sanders P
AUID- ORCID: 0000-0003-4019-480X
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Perrin-Guyomard, Agnes
AU  - Perrin-Guyomard A
AUID- ORCID: 0000-0002-4078-188X
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Hurtaud-Pessel, Dominique
AU  - Hurtaud-Pessel D
AUID- ORCID: 0000-0002-6307-6382
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Laurentie, Michel
AU  - Laurentie M
AUID- ORCID: 0000-0002-3677-5401
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Viel, Alexis
AU  - Viel A
AUID- ORCID: 0000-0002-6930-9327
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Taillandier, Jean-Francois
AU  - Taillandier JF
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Lagree, Marie-Pierre
AU  - Lagree MP
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
FAU - Gaugain, Murielle
AU  - Gaugain M
AUID- ORCID: 0000-0003-4402-0655
AD  - ANSES-Fougeres Laboratory (French National Agency for Food, Environment and 
      Occupational Health & Safety), Fougeres Cedex, France.
LA  - eng
PT  - Journal Article
DEP - 20221004
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Anti-Bacterial Agents)
RN  - 30CPC5LDEX (Sulfadimethoxine)
RN  - X20I9EN955 (Oxytetracycline)
RN  - AN164J8Y0X (Trimethoprim)
RN  - 804826J2HU (Amoxicillin)
SB  - IM
MH  - Swine
MH  - Animals
MH  - Chromatography, Liquid/methods
MH  - Anti-Bacterial Agents/analysis
MH  - Sulfadimethoxine/analysis
MH  - *Oxytetracycline/analysis
MH  - Tandem Mass Spectrometry/methods
MH  - Animal Feed/analysis
MH  - Trimethoprim/analysis
MH  - Amoxicillin/analysis
MH  - *Drug Residues/analysis
OTO - NOTNLM
OT  - Chromatography - LC-MS/MS
OT  - antibiotics
OT  - method validation
OT  - veterinary drug residues
EDAT- 2022/10/05 06:00
MHDA- 2022/11/05 06:00
CRDT- 2022/10/04 11:43
PHST- 2022/10/05 06:00 [pubmed]
PHST- 2022/11/05 06:00 [medline]
PHST- 2022/10/04 11:43 [entrez]
AID - 10.1080/19440049.2022.2126529 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 
      Nov;39(11):1818-1827. doi: 10.1080/19440049.2022.2126529. Epub 2022 Oct 4.