PMID- 36195162
OWN - NLM
STAT- MEDLINE
DCOM- 20221206
LR  - 20221208
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 193
IP  - Pt 1
DP  - 2022 Nov 20
TI  - Proteomic analysis of skeletal muscle in Chinese hamsters with type 2 diabetes 
      mellitus reveals that OPLAH downregulation affects insulin resistance and 
      impaired glucose uptake.
PG  - 23-33
LID - S0891-5849(22)00619-0 [pii]
LID - 10.1016/j.freeradbiomed.2022.09.029 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is a metabolic disease controlled by a 
      combination of genetic and environmental factors. The Chinese hamster, as a novel 
      animal model of spontaneous T2DM with high phenotypic similarity to human 
      disease, is of great value in identifying potential therapeutic targets for T2DM. 
      Here, we used tandem mass tag (TMT) quantitative proteomics based on liquid 
      chromatography-tandem mass spectrometry to assess the skeletal muscles of a 
      Chinese hamster diabetes model. We identified 38 differentially abundant 
      proteins, of which 14 were upregulated and 24 were downregulated. Further 
      analysis of the differentially abundant proteins revealed that five of them 
      (OPLAH, GST, EPHX1, SIRT5, ALDH1L1) were associated with oxidative stress; these 
      were validated at the protein and mRNA levels, and the results were consistent 
      with the proteomic analysis results. In addition, we evaluated the role of OPLAH 
      in the pathogenesis of T2DM in human skeletal muscle cells (HSKMCs) by silencing 
      it. The knockdown of OPLAH caused an increase in reactive oxygen species content, 
      decreased the GSH content, inhibited the PI3K/Akt/GLUT4 signaling pathway, and 
      reduced glucose uptake. We propose that OPLAH downregulation plays a role in 
      insulin resistance and glucose uptake disorders in HSKMCs possibly via oxidative 
      stress, making it a new therapeutic target for T2DM.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Shi, Zeya
AU  - Shi Z
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China.
FAU - Huo, Yitong
AU  - Huo Y
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China.
FAU - Hou, Jianan
AU  - Hou J
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China.
FAU - Zhang, Ruihu
AU  - Zhang R
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China.
FAU - Wu, Jianqin
AU  - Wu J
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China.
FAU - Wang, Wentao
AU  - Wang W
AD  - Department of Cardiology, The Affiliated Cardiovascular Disease Hospital of 
      Shanxi Medical University, Taiyuan, 030001, China.
FAU - Yu, Jingjing
AU  - Yu J
AD  - Experimental Animal Platform in Academy of Medical Sciences, Zhengzhou 
      University, Zhengzhou, 450052, China.
FAU - Wang, Hailong
AU  - Wang H
AD  - School of Basic Medicine, Basic Medical Science Center, Shanxi Medical 
      University, Jinzhong, 030600, China.
FAU - Liu, Yu
AU  - Liu Y
AD  - Department of Pharmacology, Shanxi Medical University, Taiyuan, 030001, China.
FAU - Song, Guohua
AU  - Song G
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China. Electronic address: ykdsgh@163.com.
FAU - Chen, Zhenwen
AU  - Chen Z
AD  - Beijing Key Laboratory of Cancer Invasion and Metastasis Research, School of 
      Basic Medical Science, Capital Medical University, Beijing, 100629, China. 
      Electronic address: czwen@ccmu.edu.cn.
FAU - Chen, Zhaoyang
AU  - Chen Z
AD  - Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science 
      and Animal Model of Human Disease, Shanxi Medical University, Taiyuan, 030001, 
      China. Electronic address: ccytycn@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221003
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (Insulin)
SB  - IM
MH  - Cricetinae
MH  - Animals
MH  - Humans
MH  - *Insulin Resistance/genetics
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Cricetulus
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Proteomics
MH  - Down-Regulation
MH  - Muscle, Skeletal/metabolism
MH  - Glucose/metabolism
MH  - Insulin/metabolism
OTO - NOTNLM
OT  - Chinese hamster
OT  - OPLAH
OT  - Proteomics
OT  - Skeletal muscle
OT  - Type 2 diabetes mellitus
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/10/05 06:00
MHDA- 2022/12/07 06:00
CRDT- 2022/10/04 19:13
PHST- 2022/06/30 00:00 [received]
PHST- 2022/09/14 00:00 [revised]
PHST- 2022/09/27 00:00 [accepted]
PHST- 2022/10/05 06:00 [pubmed]
PHST- 2022/12/07 06:00 [medline]
PHST- 2022/10/04 19:13 [entrez]
AID - S0891-5849(22)00619-0 [pii]
AID - 10.1016/j.freeradbiomed.2022.09.029 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2022 Nov 20;193(Pt 1):23-33. doi: 
      10.1016/j.freeradbiomed.2022.09.029. Epub 2022 Oct 3.