PMID- 36195213
OWN - NLM
STAT- MEDLINE
DCOM- 20221201
LR  - 20230213
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 111
DP  - 2023 Jan
TI  - Adaptation of the gut holobiont to malnutrition during mouse pregnancy depends on 
      the type of nutritional adversity.
PG  - 109172
LID - S0955-2863(22)00240-6 [pii]
LID - 10.1016/j.jnutbio.2022.109172 [doi]
AB  - Malnutrition can influence maternal physiology and programme offspring 
      development. Yet, in pregnancy, little is known about how dietary challenges that 
      influence maternal phenotype affect gut structure and function. Emerging evidence 
      suggests that interactions between the environment, multidrug resistance (MDR) 
      transporters and microbes may influence maternal adaptation to pregnancy and 
      regulate fetoplacental development. We hypothesized that the gut holobiont (host 
      and microbes) during pregnancy adapts differently to suboptimal maternal diets, 
      evidenced by changes in the gut microenvironment, morphology, and expression of 
      key protective MDR transporters during pregnancy. Mice were fed a control diet 
      (CON) during pregnancy, or undernourished (UN) by 30% of control intake from 
      gestational day (GD) 5.5-18.5, or fed 60% high fat diet (HF) for 8 weeks before 
      and during pregnancy. At GD18.5, maternal small intestinal (SI) architecture 
      (H&E), proliferation (Ki67), P-glycoprotein (P-gp - encoded by Abcb1a/b) and 
      breast cancer resistance protein (BCRP/Abcg2) MDR transporter expression and 
      levels of pro-inflammatory biomarkers were assessed. Circulating inflammatory 
      biomarkers and maternal caecal microbiome composition (G3 PhyloChip(TM)) were 
      measured. MDR transporter expression was also assessed in fetal gut. HF diet 
      increased maternal SI crypt depth and proinflammatory load, and decreased SI 
      expression of Abcb1a mRNA, whilst UN increased SI villi proliferation and Abcb1a, 
      but decreased Abcg2, mRNA expression. There were significant associations between 
      Abcb1a and Abcg2 mRNA levels with relative abundance of specific microbial taxa. 
      Using a systems physiology approach we report that common nutritional adversities 
      provoke adaptations in the pregnancy holobiont in mice, and reveal new mechanisms 
      that could influence reproductive outcomes and fetal development.
CI  - Copyright (c) 2022. Published by Elsevier Inc.
FAU - Connor, Kristin L
AU  - Connor KL
AD  - Department of Health Sciences, Carleton University, Ottawa, Ontario, Canada; 
      Mount Sinai Hospital, Lunenfeld-Tanenbaum Research Institute, Toronto, Ontario, 
      Canada. Electronic address: kristin.connor@carleton.ca.
FAU - Bloise, Enrrico
AU  - Bloise E
AD  - Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, 
      Minas Gerais, Brazil.
FAU - DeSantis, Todd Z
AU  - DeSantis TZ
AD  - Second Genome, San Francisco, California, USA.
FAU - Lye, Stephen J
AU  - Lye SJ
AD  - Mount Sinai Hospital, Lunenfeld-Tanenbaum Research Institute, Toronto, Ontario, 
      Canada; Department of Obstetrics and Gynaecology, University of Toronto, Toronto, 
      Ontario, Canada.
LA  - eng
GR  - MOP-81238/CIHR/Canada
GR  - FDN-143262/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221003
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 2)
RN  - 0 (Biomarkers)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Female
MH  - Mice
MH  - Pregnancy
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 2
MH  - Biomarkers
MH  - *Malnutrition/metabolism
MH  - Maternal Nutritional Physiological Phenomena
MH  - *Neoplasm Proteins/metabolism
MH  - RNA, Messenger
OTO - NOTNLM
OT  - Gut
OT  - Inflammation
OT  - Multidrug resistance
OT  - Nutrition
OT  - Pregnancy
EDAT- 2022/10/05 06:00
MHDA- 2022/12/01 06:00
CRDT- 2022/10/04 19:14
PHST- 2021/11/02 00:00 [received]
PHST- 2022/08/18 00:00 [revised]
PHST- 2022/08/19 00:00 [accepted]
PHST- 2022/10/05 06:00 [pubmed]
PHST- 2022/12/01 06:00 [medline]
PHST- 2022/10/04 19:14 [entrez]
AID - S0955-2863(22)00240-6 [pii]
AID - 10.1016/j.jnutbio.2022.109172 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2023 Jan;111:109172. doi: 10.1016/j.jnutbio.2022.109172. Epub 
      2022 Oct 3.