PMID- 36195392
OWN - NLM
STAT- MEDLINE
DCOM- 20221006
LR  - 20221006
IS  - 0306-4565 (Print)
IS  - 0306-4565 (Linking)
VI  - 109
DP  - 2022 Oct
TI  - ROS/JNK-mediated lysosomal injury in rat intestinal epithelial-6 cells during 
      heat stress.
PG  - 103326
LID - S0306-4565(22)00140-1 [pii]
LID - 10.1016/j.jtherbio.2022.103326 [doi]
AB  - Injury to the intestinal epithelial cells and loss of the intestinal barrier are 
      critical to heatstroke. To reveal the mechanism through which heatstroke leads to 
      intestinal epithelial injury, the relationship between reactive oxygen species 
      (ROS), c-Jun NH2-terminal kinase (JNK), and lysosomes were studied in intestinal 
      epithelial cells subjected to heat stress. Cells of heat stress groups were 
      incubated at 43  degrees C for 1 h, then incubated at 37  degrees C as indicated. Control group 
      cells were incubated at 37  degrees C. Cell-counting kit-8 assay was used to assess cell 
      viability. Cells were labeled with 2'-7'dichlorofluorescin diacetate and acridine 
      orange (AO) staining, respectively, the total ROS and AO were detected by 
      confocal laser scanning microscopy and flow cytometry. Apoptosis was analyzed by 
      flow cytometry using annexin V-fluorescein isothiocyanate/prodium iodide 
      staining, the expressions of mitogen-activated protein kinases were detected by 
      western blotting. Heat stress induced apoptosis and inhibited cell viability, the 
      production of ROS, and lysosomal injury in IEC-6 cells. After pretreatment with 
      the lysosomal cathepsin inhibitor E64, the JNK inhibitor SP600125, or the ROS 
      scavenger NAC, the effect of heat stress on apoptosis or lysosomal injury was 
      significantly attenuated. In conclusion, heat stress induced apoptosis, lysosomal 
      injury, and the accumulation of ROS in IEC-6 cells; mechanistically, this 
      occurred through the ROS-induced activation of JNK signaling, which mediated the 
      lysosomal injury and ultimately apoptosis.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Pan, Zhiguo
AU  - Pan Z
AD  - Emergency Department, Longhua Hospital Affiliated to Shanghai University of 
      Traditional Chinese Medicine, Shanghai, 200032, China; Intensive Care Unit, 
      General Hospital of Southern Theater, Guangzhou, 510010, China.
FAU - He, Xuan
AU  - He X
AD  - Intensive Care Unit, General Hospital of Southern Theater, Guangzhou, 510010, 
      China.
FAU - Shao, Yu
AU  - Shao Y
AD  - Cadre's No.7 Internal Medicine Department, General Hospital of Southern Theater, 
      Guangzhou, 510010, China.
FAU - Chen, Wenda
AU  - Chen W
AD  - Intensive Care Unit, General Hospital of Southern Theater, Guangzhou, 510010, 
      China.
FAU - Fang, Bangjiang
AU  - Fang B
AD  - Emergency Department, Longhua Hospital Affiliated to Shanghai University of 
      Traditional Chinese Medicine, Shanghai, 200032, China. Electronic address: 
      fangbj_jzk@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220915
PL  - England
TA  - J Therm Biol
JT  - Journal of thermal biology
JID - 7600115
RN  - 0 (Annexin A5)
RN  - 0 (Fluoresceins)
RN  - 0 (Iodides)
RN  - 0 (Isothiocyanates)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.- (Cathepsins)
RN  - F30N4O6XVV (Acridine Orange)
RN  - K2J09EMJ52 (Phenazopyridine)
SB  - IM
MH  - Acridine Orange/metabolism/pharmacology
MH  - Animals
MH  - Annexin A5/metabolism/pharmacology
MH  - Apoptosis
MH  - Cathepsins/metabolism/pharmacology
MH  - Epithelial Cells/metabolism
MH  - Fluoresceins/metabolism/pharmacology
MH  - *Heat Stress Disorders/metabolism
MH  - *Heat Stroke
MH  - Heat-Shock Response
MH  - *Intestinal Diseases
MH  - Iodides/metabolism/pharmacology
MH  - Isothiocyanates/metabolism/pharmacology
MH  - Lysosomes/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism/pharmacology
MH  - Phenazopyridine/metabolism/pharmacology
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Heat stress
OT  - JNK
OT  - Lysosome
OT  - ROS
COIS- Declaration of competing interest None.
EDAT- 2022/10/05 06:00
MHDA- 2022/10/07 06:00
CRDT- 2022/10/04 21:05
PHST- 2021/07/30 00:00 [received]
PHST- 2022/01/28 00:00 [revised]
PHST- 2022/09/09 00:00 [accepted]
PHST- 2022/10/04 21:05 [entrez]
PHST- 2022/10/05 06:00 [pubmed]
PHST- 2022/10/07 06:00 [medline]
AID - S0306-4565(22)00140-1 [pii]
AID - 10.1016/j.jtherbio.2022.103326 [doi]
PST - ppublish
SO  - J Therm Biol. 2022 Oct;109:103326. doi: 10.1016/j.jtherbio.2022.103326. Epub 2022 
      Sep 15.