PMID- 36197319
OWN - NLM
STAT- MEDLINE
DCOM- 20221021
LR  - 20221021
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 14
IP  - 41
DP  - 2022 Oct 19
TI  - 3D Polycaprolactone/Gelatin-Oriented Electrospun Scaffolds Promote Periodontal 
      Regeneration.
PG  - 46145-46160
LID - 10.1021/acsami.2c03705 [doi]
AB  - Periodontitis is a worldwide chronic inflammatory disease, where surgical 
      treatment still shows an uncertain prognosis. To break through the dilemma of 
      periodontal treatment, we fabricated a three-dimensional (3D) multilayered 
      scaffold by stacking and fixing electrospun polycaprolactone/gelatin (PCL/Gel) 
      fibrous membranes. The biomaterial displayed good hydrophilic and mechanical 
      properties. Besides, we found human periodontal ligament stem cell (hPDLSC) 
      adhesion and proliferation on it. The following scanning electron microscopy 
      (SEM) and cytoskeleton staining results proved the guiding function of fibers to 
      hPDLSCs. Then, we further analyzed periodontal regeneration-related proteins and 
      mRNA expression between groups. In vivo results in a rat acute periodontal defect 
      model confirmed that the topographic cues of materials could directly guide 
      cellular orientation and might provide the prerequisite for further 
      differentiation. In the aligned scaffold group, besides new bone regeneration, we 
      also observed that angular concentrated fiber regeneration in the root surface of 
      the defect is similar to the normal periodontal tissue. To sum up, we have 
      constructed electrospun membrane-based 3D biological scaffolds, which provided a 
      new treatment strategy for patients undergoing periodontal surgery.
FAU - Xu, Xuanwen
AU  - Xu X
AUID- ORCID: 0000-0001-8951-9930
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 
      Nanjing210029, China.
AD  - Jiangsu Engineering Research Center of Stomatological Translational Medicine, 
      Nanjing Medical University, Nanjing210029, China.
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing210029, China.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 
      Nanjing210029, China.
AD  - Jiangsu Engineering Research Center of Stomatological Translational Medicine, 
      Nanjing Medical University, Nanjing210029, China.
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing210029, China.
FAU - Zheng, Kai
AU  - Zheng K
AD  - Jiangsu Engineering Research Center of Stomatological Translational Medicine, 
      Nanjing Medical University, Nanjing210029, China.
FAU - Li, Xinyu
AU  - Li X
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 
      Nanjing210029, China.
AD  - Jiangsu Engineering Research Center of Stomatological Translational Medicine, 
      Nanjing Medical University, Nanjing210029, China.
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing210029, China.
FAU - Li, Lu
AU  - Li L
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 
      Nanjing210029, China.
AD  - Jiangsu Engineering Research Center of Stomatological Translational Medicine, 
      Nanjing Medical University, Nanjing210029, China.
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing210029, China.
FAU - Xu, Yan
AU  - Xu Y
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 
      Nanjing210029, China.
AD  - Jiangsu Engineering Research Center of Stomatological Translational Medicine, 
      Nanjing Medical University, Nanjing210029, China.
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing210029, China.
LA  - eng
PT  - Journal Article
DEP - 20221005
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 24980-41-4 (polycaprolactone)
RN  - 9000-70-8 (Gelatin)
RN  - 0 (Polyesters)
RN  - 0 (Biocompatible Materials)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Rats
MH  - *Gelatin
MH  - *Tissue Scaffolds
MH  - Polyesters
MH  - Biocompatible Materials/pharmacology
MH  - RNA, Messenger
MH  - Tissue Engineering/methods
OTO - NOTNLM
OT  - aligned structure
OT  - electrospinning
OT  - periodontal regeneration
OT  - three-dimensional scaffolds
OT  - tissue engineering
EDAT- 2022/10/06 06:00
MHDA- 2022/10/22 06:00
CRDT- 2022/10/05 10:23
PHST- 2022/10/06 06:00 [pubmed]
PHST- 2022/10/22 06:00 [medline]
PHST- 2022/10/05 10:23 [entrez]
AID - 10.1021/acsami.2c03705 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2022 Oct 19;14(41):46145-46160. doi: 
      10.1021/acsami.2c03705. Epub 2022 Oct 5.