PMID- 36199785
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221007
IS  - 1687-8450 (Print)
IS  - 1687-8469 (Electronic)
IS  - 1687-8450 (Linking)
VI  - 2022
DP  - 2022
TI  - The Exosomal miR-1246 of Laryngeal Squamous Cell Carcinoma Induces Polarization 
      of M2 Type Macrophages and Promotes the Invasiveness of Laryngeal Squamous Cell 
      Carcinoma.
PG  - 4424221
LID - 10.1155/2022/4424221 [doi]
LID - 4424221
AB  - BACKGROUND: The possible role and detailed mechanisms of Tumor-associated 
      macrophages (TAMs) in laryngeal squamous cell carcinoma (LSCC) have not been 
      revealed. METHODS: The expressions of typical markers were evaluated by qRT-PCR. 
      In macrophages cocultured with TU212 cells, CD163, and CD206 protein expressions 
      were detected by western blot analysis; IL-10 and IL-12 expressions were detected 
      by ELISA assay. Exosomes isolated from TU212 cells were characterized by TEM 
      analysis. As for the TU212 cells cocultured with macrophages processed with HOK 
      or TU212 cells derived exosomes, their viability, migration, and invasion were 
      assessed by CCK-8 assay, wounding healing, and Transwell assays, respectively. 
      RESULTS: In this study, macrophages processed with exosomes from human TU212 
      cells notably advanced LSCC cell viability, migration, and invasion. miR-1246 
      inhibitor suppressed the M2 polarization of macrophages. Macrophages transfected 
      with miR-1246 inhibitor suppressed LSCC cell viability, migration, and invasion. 
      CONCLUSION: In summary, our data implied that the exosomal, miR-1246 of LSCC, 
      induced polarization of M2 type macrophages and promoted the progression of LSCC. 
      This trial is registered with 2020-13.
CI  - Copyright (c) 2022 Lifeng Wu et al.
FAU - Wu, Lifeng
AU  - Wu L
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan 
      Medical College, No. 2 Zheshan West Road, Wuhu, 241001 Anhui, China.
FAU - Zuo, Na
AU  - Zuo N
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan 
      Medical College, No. 2 Zheshan West Road, Wuhu, 241001 Anhui, China.
FAU - Pan, Shuo
AU  - Pan S
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan 
      Medical College, No. 2 Zheshan West Road, Wuhu, 241001 Anhui, China.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan 
      Medical College, No. 2 Zheshan West Road, Wuhu, 241001 Anhui, China.
FAU - Wang, Qixue
AU  - Wang Q
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan 
      Medical College, No. 2 Zheshan West Road, Wuhu, 241001 Anhui, China.
FAU - Ma, Jun
AU  - Ma J
AUID- ORCID: 0000-0002-6201-0664
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan 
      Medical College, No. 2 Zheshan West Road, Wuhu, 241001 Anhui, China.
LA  - eng
PT  - Journal Article
DEP - 20220926
PL  - Egypt
TA  - J Oncol
JT  - Journal of oncology
JID - 101496537
PMC - PMC9529393
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/10/07 06:00
MHDA- 2022/10/07 06:01
PMCR- 2022/09/26
CRDT- 2022/10/06 02:13
PHST- 2022/07/22 00:00 [received]
PHST- 2022/08/18 00:00 [revised]
PHST- 2022/09/02 00:00 [accepted]
PHST- 2022/10/06 02:13 [entrez]
PHST- 2022/10/07 06:00 [pubmed]
PHST- 2022/10/07 06:01 [medline]
PHST- 2022/09/26 00:00 [pmc-release]
AID - 10.1155/2022/4424221 [doi]
PST - epublish
SO  - J Oncol. 2022 Sep 26;2022:4424221. doi: 10.1155/2022/4424221. eCollection 2022.