PMID- 36200240
OWN - NLM
STAT- MEDLINE
DCOM- 20230327
LR  - 20240223
IS  - 2211-5374 (Electronic)
VI  - 12
IP  - 1
DP  - 2023
TI  - MicroRNA-Mediated Regulation of BMP Signaling in the Developing Neural Tube.
PG  - 63-81
LID - 10.2174/2211536611666220930151322 [doi]
AB  - BACKGROUND: Neural tube (NT) morphogenesis is reliant on the proper 
      temporospatial expression of numerous genes and synchronized crosstalk between 
      diverse signaling cascades and gene regulatory networks governing key cellular 
      processes. MicroRNAs (miRNAs), a group of small non-coding regulatory RNAs, 
      execute defining roles in directing key canonical pathways during embryogenesis. 
      OBJECTIVE: In order to comprehend the mechanistic underpinnings of miRNA 
      regulation of NT morphogenesis, we have identified in the current study various 
      miRNAs and their target mRNAs associated with BMP signaling during critical 
      stages of neurulation. METHODS: We previously demonstrated the expression of 
      several miRNAs during the critical stages of neurulation (gestational days (GD) 
      8.5, 9.0, and 9.5) employing high-sensitivity, high-coverage microarrays. In the 
      present study, bioinformatic analyses were used to identify miRNAs differentially 
      expressed (DE) in the embryonic NT that target messenger RNAs (mRNAs) associated 
      with the bone morphogenetic protein (BMP) signaling pathway. RNAs extracted from 
      the developing NT were hybridized to both miRNA and mRNA arrays to evaluate 
      miRNA-mRNA interactions. RESULTS: Bioinformatic analysis identified several DE 
      miRNAs that targeted mRNAs encoding members of (and proteins associated with) the 
      BMP signaling pathway - a signaling cascade central to normal NT development. 
      CONCLUSION: Identification of the miRNAs and their mRNA targets associated with 
      BMP signaling facilitates a better understanding of the crucial epigenetic 
      mechanisms underlying normal NT development as well as the pathogenesis of NT 
      defects. The current study supports the notion that miRNAs function as key 
      regulators of neural tube morphogenesis via modulation of the BMP signaling 
      cascade. Altered expression of these miRNAs during neurulation may therefore 
      result in NT defects.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Mukhopadhyay, Partha
AU  - Mukhopadhyay P
AD  - Department of Oral Immunology and Infectious Diseases, Division of Craniofacial 
      Anomalies, University of Louisville School of Dentistry, 501 S. Preston St., 
      Louisville, KY, 40292, USA.
FAU - Seelan, Ratnam S
AU  - Seelan RS
AD  - Department of Oral Immunology and Infectious Diseases, Division of Craniofacial 
      Anomalies, University of Louisville School of Dentistry, 501 S. Preston St., 
      Louisville, KY, 40292, USA.
FAU - Greene, Robert M
AU  - Greene RM
AD  - Department of Oral Immunology and Infectious Diseases, Division of Craniofacial 
      Anomalies, University of Louisville School of Dentistry, 501 S. Preston St., 
      Louisville, KY, 40292, USA.
FAU - Pisano, M Michele
AU  - Pisano MM
AD  - Department of Oral Immunology and Infectious Diseases, Division of Craniofacial 
      Anomalies, University of Louisville School of Dentistry, 501 S. Preston St., 
      Louisville, KY, 40292, USA.
LA  - eng
GR  - R03 HD097075/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United Arab Emirates
TA  - Microrna
JT  - MicroRNA (Shariqah, United Arab Emirates)
JID - 101631045
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Neural Tube/metabolism
MH  - *MicroRNAs/genetics
MH  - Embryonic Development
MH  - Signal Transduction/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Gene Expression Profiling
OTO - NOTNLM
OT  - BMP signaling
OT  - embryonic development
OT  - etiology
OT  - miRNA
OT  - neural tube
OT  - neurulation
EDAT- 2022/10/07 06:00
MHDA- 2023/03/28 06:00
CRDT- 2022/10/06 02:54
PHST- 2022/06/15 00:00 [received]
PHST- 2022/08/24 00:00 [revised]
PHST- 2022/08/31 00:00 [accepted]
PHST- 2022/10/07 06:00 [pubmed]
PHST- 2023/03/28 06:00 [medline]
PHST- 2022/10/06 02:54 [entrez]
AID - MIRNA-EPUB-126694 [pii]
AID - 10.2174/2211536611666220930151322 [doi]
PST - ppublish
SO  - Microrna. 2023;12(1):63-81. doi: 10.2174/2211536611666220930151322.