PMID- 36202902
OWN - NLM
STAT- MEDLINE
DCOM- 20221010
LR  - 20221202
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Oct 6
TI  - Magnetic nanowires substrate increases adipose-derived mesenchymal cells 
      osteogenesis.
PG  - 16698
LID - 10.1038/s41598-022-21145-z [doi]
LID - 16698
AB  - Magnetic nanomaterials are increasingly impacting the field of biology and 
      medicine. Their versatility in terms of shape, structure, composition, coating, 
      and magnetic responsivity make them attractive for drug delivery, cell targeting 
      and imaging. Adipose derived-mesenchymal cells (ASCs) are intensely scrutinized 
      for tissue engineering and regenerative medicine. However, differentiation into 
      musculoskeletal lineages can be challenging. In this paper, we show that uncoated 
      nickel nanowires (Ni NW) partially released from their alumina membrane offer a 
      mechanically-responsive substrate with regular topography that can be used for 
      the delivery of magneto-mechanical stimulation. We have used a tailored protocol 
      for improving ASCs adherence to the substrate, and showed that cells retain their 
      characteristic fibroblastic appearance, cytoskeletal fiber distribution and good 
      viability. We report here for the first time significant increase in osteogenic 
      but not adipogenic differentiation of ASCs on Ni NW exposed to 4 mT magnetic 
      field compared to non-exposed. Moreover, magnetic actuation is shown to induce 
      ASCs osteogenesis but not adipogenesis in the absence of external biochemical 
      cues. While these findings need to be verified in vivo, the use of Ni NW 
      substrate for inducing osteogenesis in the absence of specific differentiation 
      factors is attractive for bone engineering. Implant coating with similar surfaces 
      for orthopedic and dentistry could be as well envisaged as a modality to improve 
      osteointegration.
CI  - (c) 2022. The Author(s).
FAU - Labusca, Luminita
AU  - Labusca L
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
AD  - Orthopedics and Traumatology Clinic, County Emergency Hospital Saint Spiridon 
      Iasi, 700111, Iasi, Romania.
FAU - Danceanu, Camelia
AU  - Danceanu C
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Minuti, Anca Emanuela
AU  - Minuti AE
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
AD  - Alexandru Ioan Cuza University, Faculty of Physics, 700506, Iasi, Romania.
FAU - Herea, Dumitru-Daniel
AU  - Herea DD
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania. dherea@phys-iasi.ro.
FAU - Ghemes, Adrian
AU  - Ghemes A
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Rotarescu, Cristian
AU  - Rotarescu C
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Dragos-Pinzaru, Oana
AU  - Dragos-Pinzaru O
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Tibu, Mihai
AU  - Tibu M
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Marian, Grigoras
AU  - Marian G
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Chiriac, Horia
AU  - Chiriac H
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
FAU - Lupu, Nicoleta
AU  - Lupu N
AD  - Department of Magnetic Devices and Materials, National Institute of Research and 
      Development for Technical Physics, 700050, Iasi, Romania.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221006
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 7OV03QG267 (Nickel)
RN  - LMI26O6933 (Aluminum Oxide)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Aluminum Oxide
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Magnetic Phenomena
MH  - *Nanowires
MH  - Nickel/metabolism
MH  - *Osteogenesis
PMC - PMC9537172
COIS- The authors declare no competing interests.
EDAT- 2022/10/07 06:00
MHDA- 2022/10/12 06:00
PMCR- 2022/10/06
CRDT- 2022/10/06 23:26
PHST- 2022/06/24 00:00 [received]
PHST- 2022/09/22 00:00 [accepted]
PHST- 2022/10/06 23:26 [entrez]
PHST- 2022/10/07 06:00 [pubmed]
PHST- 2022/10/12 06:00 [medline]
PHST- 2022/10/06 00:00 [pmc-release]
AID - 10.1038/s41598-022-21145-z [pii]
AID - 21145 [pii]
AID - 10.1038/s41598-022-21145-z [doi]
PST - epublish
SO  - Sci Rep. 2022 Oct 6;12(1):16698. doi: 10.1038/s41598-022-21145-z.