PMID- 36203120
OWN - NLM
STAT- MEDLINE
DCOM- 20221024
LR  - 20221024
IS  - 2299-5684 (Electronic)
IS  - 1734-1140 (Linking)
VI  - 74
IP  - 5
DP  - 2022 Oct
TI  - Simultaneous determination of four antibiotics in human urine and hair samples 
      employing a simple and efficient dispersive micro solid-phase extraction-high 
      performance liquid chromatography.
PG  - 1069-1082
LID - 10.1007/s43440-022-00404-w [doi]
AB  - BACKGROUND: Antibiotics containing azithromycin, amoxicillin, doxycycline, 
      tetracycline, and azithromycin are widely utilized in the treatment of bacterial 
      infections, and their determination in biological samples is critical to evaluate 
      their side effects. METHODS: A dispersive micro solid-phase extraction (DMSPE) as 
      an efficient sample preparation was developed for the simultaneous extraction of 
      the antibiotics in biological samples. For this purpose, chitosan@Fe(3)O(4) 
      nanoparticles were prepared as a green and magnetic sorbent based on a chemical 
      coating of Fe(3)O(4) nanoparticles with chitosan. RESULTS: The sorbent was 
      compared with several sorbents such as ZnO NPs, CuO NPs, and Fe(3)O(4) NPs to 
      extract the antibiotics. In addition, effective factors for antibiotic extraction 
      were evaluated and optimized using a central composite design and one factor at a 
      time. The method has wide linearity in the concentration ranges of 2.1-800.0, 
      3.5-1000.0, 2.0-800.0, and 2.7-1000.0 mug L(-1) with an R-squared higher than 
      0.9958 for the determination of azithromycin, amoxicillin, doxycycline, and 
      tetracycline, respectively. The limit of detection and limit of quantitation was 
      lower than 1.1 and 3.5 mug L(-1), respectively. The relative standard deviations 
      (RSDs) were also lower than 4.90% for the determination of the analytes at a 
      concentration of 20.0 microg L(-1). High recoveries in the percentage range of 
      94.70-106.75%, with RSDs between 4.64 and 5.83%, were obtained to analyze spiked 
      urine and hair samples. CONCLUSIONS: The DMSPE-HPLC-DAD procedure displayed 
      various advantages for determining antibiotics, including simple operation, high 
      recoveries, low sample solution and desorption solvent consumption, high 
      preconcentration factors, proper determination time, and simple and inexpensive 
      method of sorbent preparation.
CI  - (c) 2022. The Author(s) under exclusive licence to Maj Institute of Pharmacology 
      Polish Academy of Sciences.
FAU - Soleimanirad, Ehsan
AU  - Soleimanirad E
AD  - Department of Chemistry, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, P.O. Box 91735-413, Mashhad, Iran.
FAU - Badameh, Anousheh
AU  - Badameh A
AD  - Department of Chemistry, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, P.O. Box 91735-413, Mashhad, Iran.
FAU - Raoufi, Arastou
AU  - Raoufi A
AD  - Department of Chemistry, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, P.O. Box 91735-413, Mashhad, Iran. a.raoufi@mshdiau.ac.ir.
FAU - Ebrahimi, Mahmoud
AU  - Ebrahimi M
AD  - Department of Chemistry, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, P.O. Box 91735-413, Mashhad, Iran. Ebrachem2007@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20221007
PL  - Switzerland
TA  - Pharmacol Rep
JT  - Pharmacological reports : PR
JID - 101234999
RN  - 0 (Anti-Bacterial Agents)
RN  - 83905-01-5 (Azithromycin)
RN  - N12000U13O (Doxycycline)
RN  - 9012-76-4 (Chitosan)
RN  - SOI2LOH54Z (Zinc Oxide)
RN  - 0 (Solvents)
RN  - 804826J2HU (Amoxicillin)
SB  - IM
MH  - Humans
MH  - Chromatography, High Pressure Liquid/methods
MH  - Anti-Bacterial Agents
MH  - Azithromycin
MH  - Doxycycline
MH  - *Chitosan
MH  - *Zinc Oxide
MH  - Solid Phase Extraction/methods
MH  - Solvents/chemistry
MH  - Amoxicillin
MH  - Hair
MH  - Limit of Detection
OTO - NOTNLM
OT  - Antibiotic
OT  - Central composite design
OT  - Chitosan
OT  - Dispersive micro solid-phase extraction (DMSE)
OT  - Human hair sample
OT  - Magnetic Fe3O4 nanoparticles
EDAT- 2022/10/07 06:00
MHDA- 2022/10/25 06:00
CRDT- 2022/10/06 23:39
PHST- 2022/04/19 00:00 [received]
PHST- 2022/08/01 00:00 [accepted]
PHST- 2022/07/31 00:00 [revised]
PHST- 2022/10/07 06:00 [pubmed]
PHST- 2022/10/25 06:00 [medline]
PHST- 2022/10/06 23:39 [entrez]
AID - 10.1007/s43440-022-00404-w [pii]
AID - 10.1007/s43440-022-00404-w [doi]
PST - ppublish
SO  - Pharmacol Rep. 2022 Oct;74(5):1069-1082. doi: 10.1007/s43440-022-00404-w. Epub 
      2022 Oct 7.