PMID- 36204098
OWN - NLM
STAT- MEDLINE
DCOM- 20221104
LR  - 20230421
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - Metabolomics analysis of stool in rats with type 2 diabetes mellitus after 
      single-anastomosis duodenal-ileal bypass with sleeve gastrectomy.
PG  - 1013959
LID - 10.3389/fendo.2022.1013959 [doi]
LID - 1013959
AB  - BACKGROUND: Single-anastomosis duodenal-ileal bypass with sleeve gastrectomy 
      (SADI-S) is one of the most effective bariatric procedures in the treatment of 
      type 2 diabetes mellitus (T2DM). However, the mechanisms by which SADI-S improves 
      T2DM are not well-known. OBJECTIVE: To explore the effects of SADI-S on 
      metabolites in the stool of rats with T2DM. METHODS: Twenty rats were fed on 
      high-fat diet and administered with a low-dose (30mg/kg) of streptozotocin to 
      establish T2DM models. The rats were then randomly assigned to the SADI-S group 
      (n=10) and sham operation group (n=9). Stool samples were collected from all rats 
      at 8 weeks after surgery and stored at -80  degrees C. Metabolomics analysis was 
      performed to identify differential metabolites through ultra- performance liquid 
      chromatography-mass spectrometry. RESULTS: At 8-week after surgery, rats of the 
      SADI-S group showed significantly decreased fasting blood glucose, glucose 
      tolerance test 2-hour, glycated haemoglobin, and body weight compared with those 
      of the sham group. A total of 245 differential metabolites were identified 
      between the two groups. Among them, 16 metabolites such as branched-chain amino 
      acids (valine), aromatic amino acid (phenylalanine), bile acid (cholic acid, 
      lithocholic acid, and beta-muricholic acid), short-chain fatty acid (isobutyric 
      acid), and phospholipid [lysoPE(17:0), lysoPE(20:3) and lysoPS(16:0)] were 
      associated to the T2DM remission after SADI-S. CONCLUSION: SADI-S improves T2DM 
      in rats by regulating phenylalanine biosynthesis, valine, phenylalanine, alanine, 
      glutamate, proline, bile acid, and phospholipid metabolism pathways.
CI  - Copyright (c) 2022 Wang, Wang, Yu, Ren, Jia, Wang, Li and Jiang.
FAU - Wang, Lun
AU  - Wang L
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Wang, Zeyu
AU  - Wang Z
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Yu, Yang
AU  - Yu Y
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Ren, Zhaoheng
AU  - Ren Z
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Jia, Yongheng
AU  - Jia Y
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Wang, Jinfa
AU  - Wang J
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Li, Shixing
AU  - Li S
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of 
      Jilin University, Changchun City, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220920
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - OF5P57N2ZX (Alanine)
RN  - 0 (Amino Acids, Aromatic)
RN  - 0 (Amino Acids, Branched-Chain)
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Blood Glucose)
RN  - 0 (Glutamates)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Isobutyrates)
RN  - 5QU0I8393U (Lithocholic Acid)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 0 (Phospholipids)
RN  - 9DLQ4CIU6V (Proline)
RN  - 5W494URQ81 (Streptozocin)
RN  - HG18B9YRS7 (Valine)
SB  - IM
EIN - Front Endocrinol (Lausanne). 2023 Mar 28;14:1128339. PMID: 37082128
MH  - Animals
MH  - Rats
MH  - Alanine
MH  - Amino Acids, Aromatic
MH  - Amino Acids, Branched-Chain
MH  - Anastomosis, Surgical/methods
MH  - Bile Acids and Salts
MH  - Blood Glucose/analysis
MH  - *Diabetes Mellitus, Type 2/surgery
MH  - Gastrectomy/methods
MH  - Glutamates
MH  - Glycated Hemoglobin/analysis
MH  - Ileum/chemistry/surgery
MH  - Isobutyrates
MH  - Lithocholic Acid
MH  - *Obesity, Morbid/surgery
MH  - Phenylalanine
MH  - Phospholipids
MH  - Proline
MH  - Streptozocin
MH  - Valine
PMC - PMC9530139
OTO - NOTNLM
OT  - SADI-S
OT  - T2DM
OT  - bariatric surgery
OT  - metabolomics
OT  - single-anastomosis duodenal-ileal bypass with sleeve gastrectomy
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/10/08 06:00
MHDA- 2022/10/12 06:00
PMCR- 2022/01/01
CRDT- 2022/10/07 02:46
PHST- 2022/08/08 00:00 [received]
PHST- 2022/08/29 00:00 [accepted]
PHST- 2022/10/07 02:46 [entrez]
PHST- 2022/10/08 06:00 [pubmed]
PHST- 2022/10/12 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.1013959 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Sep 20;13:1013959. doi: 
      10.3389/fendo.2022.1013959. eCollection 2022.