PMID- 36204450
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221009
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 16
DP  - 2022
TI  - Galectin-3 administration drives remyelination after hypoxic-ischemic induced 
      perinatal white matter injury.
PG  - 976002
LID - 10.3389/fncel.2022.976002 [doi]
LID - 976002
AB  - Hypoxic-ischemic (HI) induced perinatal white matter injury (PWMI) is a major 
      cause of neurologic disabilities characterized by selective oligodendroglial 
      death and myelin disruption. Galectin-3 (Gal-3) modulates postnatal 
      subventricular zone gliogenesis and attenuates ischemic injury. However, the 
      association between Gal-3 and myelin formation still remains unclear. In this 
      study, we first perform Gal-3 knockdown (KD) to identify the importance of Gal-3 
      on myelin formation. Our results show impeded myelin formation, manifested by 
      Olig2/CC1 (+) mature oligodendrocytes number, expression of oligodendroglial 
      maturation-associated markers (MBP and CNPase), and myelin thickness and 
      integrity. Then we perform recombinant Gal-3 (rGal-3) administration by 
      intracerebroventricular injection. Notably, although rGal-3 administration shows 
      no beneficial effect on oligodendrogenesis and myelin formation under normal 
      condition, our results show that rGal-3 administration attenuates cognitive 
      deficits and drives remyelination after PWMI, which are coupled to signs of 
      enhanced myelin resiliency and cognition. Also, our results indicates that the 
      significant increases in substrates for remyelination of rGal-3 administration 
      are accompanied by enhanced Iba-1 (microglia marker)/ Mrc1 (M2 marker) (+) 
      microglia and decreased Iba-1/ iNOS (M1 marker) (+) microglia. Altogether, our 
      data in this research confirm the association between Gal-3 and myelin formation, 
      underscore its position for the capacity for remyelination and restoration of 
      function, and unveils the efficacy of rGal-3 administration with 
      anti-inflammatory phenotype microglia (M2 microglia) activation. Thus, the 
      findings suggest that Gal-3 plays a significant role in myelin formation and 
      remyelination restoration.
CI  - Copyright (c) 2022 Wang, Diao, Qiu, Gao, Wang, Chen, Xiao, Li and Chen.
FAU - Wang, Qian
AU  - Wang Q
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
AD  - Department of Neonatology, Women and Children's Medical Center of Guangzhou, 
      Guangzhou, China.
FAU - Diao, Sihao
AU  - Diao S
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
FAU - Qiu, Han
AU  - Qiu H
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
FAU - Gao, Ruiwei
AU  - Gao R
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
FAU - Wang, Minjie
AU  - Wang M
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
FAU - Chen, Qiufan
AU  - Chen Q
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
AD  - Department of Neonatology, Women and Children's Medical Center of Guangzhou, 
      Guangzhou, China.
FAU - Xiao, Mili
AU  - Xiao M
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
FAU - Li, Zhihua
AU  - Li Z
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
FAU - Chen, Chao
AU  - Chen C
AD  - Department of Neonatology, Children's Hospital of Fudan University, Shanghai, 
      China.
AD  - Key Laboratory of Neonatal Diseases, National Health Commission, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20220920
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC9532057
OTO - NOTNLM
OT  - M2 microglia
OT  - galectin-3
OT  - myelin formation
OT  - oligodendrocytes
OT  - perinatal white matter injury
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/10/08 06:00
MHDA- 2022/10/08 06:01
PMCR- 2022/01/01
CRDT- 2022/10/07 02:53
PHST- 2022/06/23 00:00 [received]
PHST- 2022/08/31 00:00 [accepted]
PHST- 2022/10/07 02:53 [entrez]
PHST- 2022/10/08 06:00 [pubmed]
PHST- 2022/10/08 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2022.976002 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2022 Sep 20;16:976002. doi: 10.3389/fncel.2022.976002. 
      eCollection 2022.