PMID- 3620476
OWN - NLM
STAT- MEDLINE
DCOM- 19870930
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 914
IP  - 3
DP  - 1987 Aug 21
TI  - alpha-D-mannopyranosylmethyl-p-nitrophenyltriazene inhibition of rat liver 
      alpha-D-mannosidases.
PG  - 283-8
AB  - The compound alpha-D-mannopyranosylmethyl-p-nitrophenyltriazene (alpha-ManMNT) 
      has been tested for its effect on four alpha-D-mannosidase activities present in 
      rat liver. When p-nitrophenyl alpha-D-mannopyranoside was used as a substrate, 
      preincubation of enzyme with 1.0 mM alpha-ManMNT inhibited soluble 
      alpha-D-mannosidase by 90%, lysosomal alpha-D-mannosidase by approx. 60%, and had 
      virtually no effect on Golgi mannosidase II. Golgi mannosidase I removal of the 
      four alpha-1,2-linked D-mannoses from the common Man9GlcNAc2 oligosaccharide 
      structure formed during N-linked glycoprotein biosynthesis was also blocked by 
      treatment of the Golgi fraction with this compound. Mannosyltriazene inhibition 
      of the three susceptible hepatic alpha-D-mannosidases was largely irreversible. 
      alpha-ManMNT should therefore be useful for studying oligosaccharide processing 
      and possibly for determining the turnover time of the inhibited 
      alpha-D-mannosidases.
FAU - Docherty, P A
AU  - Docherty PA
FAU - Aronson, N N Jr
AU  - Aronson NN Jr
LA  - eng
GR  - DK 33314-04/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Triazenes)
RN  - 101396-17-2 (mannopyranosylmethyl-4-nitrophenyltriazene)
RN  - EC 3.2.1.- (Mannosidases)
RN  - EC 3.2.1.113 (mannosyl-oligosaccharide 1,2-alpha-mannosidase)
RN  - EC 3.2.1.114 (mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase)
SB  - IM
MH  - Animals
MH  - Cytosol/enzymology
MH  - Golgi Apparatus/enzymology
MH  - Kinetics
MH  - Liver/*enzymology
MH  - Lysosomes/enzymology
MH  - Mannosidases/*antagonists & inhibitors
MH  - Rats
MH  - Triazenes/*pharmacology
EDAT- 1987/08/21 00:00
MHDA- 2001/03/28 10:01
CRDT- 1987/08/21 00:00
PHST- 1987/08/21 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1987/08/21 00:00 [entrez]
AID - 0167-4838(87)90287-1 [pii]
AID - 10.1016/0167-4838(87)90287-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1987 Aug 21;914(3):283-8. doi: 
      10.1016/0167-4838(87)90287-1.