PMID- 3620552
OWN - NLM
STAT- MEDLINE
DCOM- 19870929
LR  - 20180216
IS  - 0006-3126 (Print)
IS  - 0006-3126 (Linking)
VI  - 52
IP  - 1
DP  - 1987
TI  - Effect of dexamethasone on the adenylate cyclase system of cultured hepatocytes 
      of fetal rats.
PG  - 33-9
AB  - During treatment of primary cultured hepatocytes with dexamethasone for several 
      hours, cyclic AMP formation and glycolysis by glucagon increased dose 
      dependently. In the cells pretreated with dexamethasone, the output of 
      intracellular cyclic AMP increased significantly (p less than 0.01) with glucagon 
      of 2.8 X 10(-9) M or more, and the amount of glucose released also increased 
      significantly (p less than 0.01) with glucagon of 2.8 X 10(-8) M or more, 
      compared to the control cells. Moreover, treatment with dexamethasone increased 
      the stimulatory effect of guanosine-5'-triphosphate (GTP), 
      guanyl-5'-yl-imidodiphosphate on adenylate cyclase and significantly increased 
      the stimulatory effect of fluoride. In the rat hepatocytes primarily cultured 
      with dexamethasone for several hours, the stimulatory effect of GTP and fluoride 
      on adenylate cyclase increased time dependently. These data indicate that the 
      glucocorticoid regulates the sensitivity of adenylate cyclase at distinct loci of 
      the postreceptor system.
FAU - Nishida, N
AU  - Nishida N
FAU - Takeuchi, T
AU  - Takeuchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Biol Neonate
JT  - Biology of the neonate
JID - 0247551
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Cells, Cultured
MH  - Dexamethasone/administration & dosage/*pharmacology
MH  - Drug Synergism
MH  - Enzyme Activation
MH  - Guanosine Triphosphate/administration & dosage
MH  - Guanylyl Imidodiphosphate/administration & dosage
MH  - Liver/*enzymology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sodium Fluoride/administration & dosage
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1159/000242682 [doi]
PST - ppublish
SO  - Biol Neonate. 1987;52(1):33-9. doi: 10.1159/000242682.