PMID- 362066
OWN - NLM
STAT- MEDLINE
DCOM- 19790124
LR  - 20131121
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 39
IP  - 3
DP  - 1978 Sep
TI  - Morphology of the early hemostasis in human skin wounds: influence of 
      acetylsalicylic acid.
PG  - 298-311
AB  - The in vivo formation of hemostatic plugs was studied in humans in skin wounds 
      made using the template bleeding procedure of Mielke (34). The wounds were 
      excised by punch biopsy 10 seconds, 30 seconds, 2 minutes, and 3 minutes after 
      they were made. The wounds were V-shaped and approximately 0.4 mm. deep. Within 
      30 seconds small hemostatic plugs were observed at the end of transected vessels. 
      The plugs grew in size in the subsequent minutes and became impermeable. The 
      platelets degranulated and formed pseudopods which became strongly 
      interdigitated. The platelets at the periphery of the plugs showed 
      discontinuities of the membranes. Cytoplasmic matrix and cell organelles had 
      disappeared in many of these peripheral cells. Small fibrin fibers were already 
      found at 30 seconds, mostly along the margins of the wounds and also at the 
      periphery of the hemostatic plugs. Fibrin was absent from the center of the plugs 
      and from the lumen of transected vessels. When part of a plug was extending into 
      the vessel lumen, the platelets inside the vessel were less degranulated and less 
      interdigitated than the rest of the plug. The effect of acetysalicylic acid (ASA) 
      was studied in wounds before and 2.5 hours after ingestion of 2 gm. of ASA. 
      Wounds were excised by punch biopsy 3 or 10 minutes after they had been made. 
      Platelets in ASA were less degranulated, had fewer pseudopods, and showed less 
      interdigitation than platelets in control plugs. Ballooning and fibrin deposition 
      were similar in control and ASA plugs. Pronounced differences between control and 
      ASA plugs were observed in a subject who exhibited a considerably prolonged 
      bleeding time after ASA. The ASA plugs were very large; many plugs had fused and 
      in addition numerous small platelet clumps, most likely fragments from the plugs, 
      were found in the superficial scab of the wound. It is postulated that ASA plugs 
      are less stable due to decreased interdigitation. This allows more disruption of 
      the plugs and rebleeding. Consequently, more platelets are needed and longer time 
      is required for hemostasis to occur.
FAU - Wester, J
AU  - Wester J
FAU - Sixma, J J
AU  - Sixma JJ
FAU - Geuze, J J
AU  - Geuze JJ
FAU - van der Veen, J
AU  - van der Veen J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 9001-31-4 (Fibrin)
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Adult
MH  - Aspirin/*pharmacology
MH  - Blood Platelets/ultrastructure
MH  - Cell Membrane/ultrastructure
MH  - Fibrin/metabolism
MH  - Hemostasis/*drug effects
MH  - Humans
MH  - Male
MH  - Microscopy, Electron
MH  - Pseudopodia/ultrastructure
MH  - Skin/*injuries
MH  - Time Factors
MH  - Wounds, Penetrating/*blood/metabolism/pathology
RF  - 65
EDAT- 1978/09/01 00:00
MHDA- 1978/09/01 00:01
CRDT- 1978/09/01 00:00
PHST- 1978/09/01 00:00 [pubmed]
PHST- 1978/09/01 00:01 [medline]
PHST- 1978/09/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1978 Sep;39(3):298-311.