PMID- 36206670
OWN - NLM
STAT- MEDLINE
DCOM- 20221024
LR  - 20221024
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 218
DP  - 2022 Dec 15
TI  - Reduced graphene oxide increases cells with enlarged outer membrane of 
      Citrifermentans bremense and exopolysaccharides secretion.
PG  - 114754
LID - S0956-5663(22)00794-1 [pii]
LID - 10.1016/j.bios.2022.114754 [doi]
AB  - Conductive carbons can boost anaerobic microbial metabolism by assisting 
      extracellular electron transfer (EET), and their chemistry affects microbial 
      metabolism. Graphene oxide (GO), a chemically oxidized sheet of graphite, has 
      been used in various bioelectrochemical systems, although its mechanism is rarely 
      understood. This study revealed specific metabolic responses to reduced GO (rGO) 
      in an electrogenic strain R4 of Citrifermentans bremense, recently renamed from 
      "Geobacter bremensis," in comparison to that with graphite felt (GF). 
      Specifically, the change in growth from planktonic cells to biofilm with an 
      enlarged outer membrane. The mRNA profile supported the fact that rGO upregulated 
      the 14 genes related to the exopolysaccharides (EPS) secretion and biofilm 
      formation, which is more than that in GF (4 genes). While GF upregulated the 35 
      genes involved in cell motility, which is more than that in rGO (8 genes). The 
      heme protein profile suggested that both carbons induced similar EET pathways 
      involving OmcA/MtrC and OmcS; however, GO specifically induced PilQ. These 
      findings show that the chemistry of conductive carbon differentiates metabolism, 
      especially affecting cellular morphology or living form, rather than electron 
      transfer metabolism.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Meng, Lingyu
AU  - Meng L
AD  - Department of Civil Engineering, Nagoya Institute of Technology, Nagoya, Aichi, 
      Japan.
FAU - Xie, Li
AU  - Xie L
AD  - Department of Civil Engineering, Nagoya Institute of Technology, Nagoya, Aichi, 
      Japan.
FAU - Hirose, Yuu
AU  - Hirose Y
AD  - Department of Environmental and Life Sciences, Toyohashi University of 
      Technology, Toyohashi, Aichi, Japan; Electronics-Inspired Interdisciplinary 
      Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, 
      Aichi, Japan.
FAU - Nishiuchi, Takumi
AU  - Nishiuchi T
AD  - Bioscience Core Facility, Research Center for Experimental Modeling of Human 
      Disease, Kanazawa University, Kanazawa, Ishikawa, Japan.
FAU - Yoshida, Naoko
AU  - Yoshida N
AD  - Department of Civil Engineering, Nagoya Institute of Technology, Nagoya, Aichi, 
      Japan. Electronic address: yoshida.naoko@nitech.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20221001
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - 7440-44-0 (Carbon)
RN  - 0 (RNA, Messenger)
RN  - 0 (Hemeproteins)
SB  - IM
MH  - *Graphite
MH  - *Biosensing Techniques
MH  - Carbon
MH  - RNA, Messenger
MH  - *Hemeproteins
OTO - NOTNLM
OT  - Biofilm formation
OT  - Cell motility
OT  - Exoelectrogenic bacteria
OT  - Genome-wide RNA sequencing
OT  - Graphene oxide
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/10/08 06:00
MHDA- 2022/10/25 06:00
CRDT- 2022/10/07 18:22
PHST- 2022/08/07 00:00 [received]
PHST- 2022/09/16 00:00 [revised]
PHST- 2022/09/23 00:00 [accepted]
PHST- 2022/10/08 06:00 [pubmed]
PHST- 2022/10/25 06:00 [medline]
PHST- 2022/10/07 18:22 [entrez]
AID - S0956-5663(22)00794-1 [pii]
AID - 10.1016/j.bios.2022.114754 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2022 Dec 15;218:114754. doi: 10.1016/j.bios.2022.114754. 
      Epub 2022 Oct 1.