PMID- 36210640
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230907
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 56
IP  - 2
DP  - 2023 Feb
TI  - Periplaneta Americana extract inhibits osteoclastic differentiation in vitro.
PG  - e13341
LID - 10.1111/cpr.13341 [doi]
LID - e13341
AB  - OBJECTIVES: Periplaneta americana extract (PAE) is proven to be promising in 
      treating fever, wound healing, liver fibrosis, and cardiovascular disease. 
      However, the role of PAE in skeletal disorders remains unclear. This study 
      investigated whether PAE regulates osteoclastic differentiation in vitro via the 
      culture using RAW264.7 cells and bone marrow derived macrophages (BMDMs). 
      MATERIALS AND METHODS: RAW264.7 cells and BMDMs were cultured and induced for 
      osteoclastic differentiation supplementing with different concentrations of PAE 
      (0, 0.1, 1, and 10 mg/mL). Cell counting kit-8 (CCK-8) assay was used to detect 
      the cytotoxicity and cell proliferation. TRAP staining, actin ring staining, 
      real-time quantitative PCR (RT-qPCR), and bone resorption activity test were 
      performed to detect osteoclastic differentiation. RT-qPCR and enzyme-linked 
      immunosorbent assay (ELISA) were conducted to assay the expression and secretion 
      of inflammatory factors. RNA sequencing (RNA-seq) and western blot analysis were 
      carried out to uncover the underlying mechanism. RESULTS: CCK-8 results showed 
      that 10 mg/mL and a lower concentration of PAE did not affect cell proliferation. 
      RT-qPCR analysis verified that PAE down-regulated the osteoclastic genes Nfatc1, 
      Ctsk, and Acp5 in macrophages. Moreover, PAE restrained the differentiation, 
      formation, and function of osteoclasts. Besides, RT-qPCR and ELISA assays showed 
      that PAE decreased inflammatory genes expression and reduced the secretion of 
      inflammatory factors, including IL1beta, IL6, and TNFalpha. Subsequent RNA-seq analysis 
      identified possible genes and signaling pathways of PAE-mediated 
      osteoclastogenesis suppression. CONCLUSIONS: Our study indicates that PAE has 
      inhibitive effects on osteoclastogenesis and may be a potential therapeutic 
      alternative for bone diseases.
CI  - (c) 2022 The Authors. Cell Proliferation published by Beijing Institute for Stem 
      Cell and Regenerative Medicine and John Wiley & Sons Ltd.
FAU - Zhao, Bin
AU  - Zhao B
AUID- ORCID: 0000-0003-4365-3821
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral 
      Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Zhang, Yuning
AU  - Zhang Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral 
      Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Xu, Jie
AU  - Xu J
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral 
      Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Li, Yuyu
AU  - Li Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral 
      Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Yuan, Quan
AU  - Yuan Q
AUID- ORCID: 0000-0002-2836-1081
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral 
      Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
AD  - Department of Oral Implantology, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, China.
FAU - Zhou, Chenchen
AU  - Zhou C
AUID- ORCID: 0000-0002-6427-5869
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral 
      Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
AD  - Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, China.
LA  - eng
GR  - 81901040/National Natural Science Foundation of China/
GR  - 82171001/National Natural Science Foundation of China/
GR  - 2020QNR00/Young Elite Scientist Sponsorship Program by CAST/
PT  - Journal Article
DEP - 20221009
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (RANK Ligand)
SB  - IM
MH  - Animals
MH  - *Periplaneta/metabolism
MH  - Osteoclasts/metabolism
MH  - Macrophages/metabolism
MH  - Osteogenesis/physiology
MH  - Cell Differentiation/physiology
MH  - RANK Ligand/metabolism
PMC - PMC9890529
COIS- All authors declare that there is no conflict of interest.
EDAT- 2022/10/11 06:00
MHDA- 2023/02/03 06:00
PMCR- 2022/10/09
CRDT- 2022/10/10 02:44
PHST- 2022/09/01 00:00 [revised]
PHST- 2022/05/28 00:00 [received]
PHST- 2022/09/07 00:00 [accepted]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/10/10 02:44 [entrez]
PHST- 2022/10/09 00:00 [pmc-release]
AID - CPR13341 [pii]
AID - 10.1111/cpr.13341 [doi]
PST - ppublish
SO  - Cell Prolif. 2023 Feb;56(2):e13341. doi: 10.1111/cpr.13341. Epub 2022 Oct 9.