PMID- 36212957
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221011
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2022
DP  - 2022
TI  - The Mitigatory Effect of Shen-Qi Compound on the Diabetic Thoracic Aortic 
      Complications through Inhibiting the Inflammatory Microenvironment by 
      miR-223-3p/RBP-J/IRF8 Axis.
PG  - 6686931
LID - 10.1155/2022/6686931 [doi]
LID - 6686931
AB  - BACKGROUND: Disruption of the vascular immunological inflammatory 
      microenvironment is linked to metabolic memory impairment. Even though it has 
      been proven that the Shen-Qi compound (SQC) can efficiently halt metabolic memory 
      and preserve vascular endothelial cells, extensive studies need to be done to 
      investigate if it can also change the vascular immune-inflammatory 
      microenvironment by regulating the immune system. This will help figure out the 
      role of stopping metabolic memory. METHODS: After 4 weeks on a high-fat diet 
      (HFD), GK rats were used to create a model for diabetic thoracic aortic problems. 
      The effect and mechanisms of SQC on diabetic thoracic aortic complications were 
      assessed by hematoxylin-eosin (H&E) staining, enzyme-linked immunosorbent assay 
      (ELISA), biochemical analysis, terminal deoxynucleotidyl transferase deoxyuridine 
      triphosphate (dUTP) nick end labeling (TUNEL), reverse transcription, real-time 
      polymerase chain reaction (RT-qPCR), immunofluorescence (IF), western blot, and 
      luciferase reporter assays. RESULTS: SQC treatment ameliorates the HFD-induced 
      pathological symptoms as well as the HFD-induced increased concentrations of 
      fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), 
      triglycerides (TGs), and low-density lipoprotein cholesterol (LDL-C) and 
      decreased concentrations of high-density lipoprotein cholesterol (HDL-C). 
      Besides, SQC counteracted the HFD-induced average fluorescence intensity of 
      vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 
      (ICAM-1), as well as the concentrations of endothelin-1 (ET-1) and monocyte 
      chemoattractant protein-1 (MCP-1), while rescuing the HFD-induced concentrations 
      of nitric oxide (NO) and nitric oxide synthetase (NOS). Also, SQC decreases 
      apoptosis and oxidative stress in rats with diabetic thoracic aortic 
      complications. In addition, SQC facilitated the polarization of macrophages, 
      stimulated the activation of dendritic cells, and regulated the inflammatory 
      milieu in rats with diabetic thoracic aortic complications. Furthermore, SQC also 
      modulated the miR-223-3p/RBP-J/IRF8 axis in the macrophages of rats with diabetic 
      thoracic aortic complications. CONCLUSION: SQC ameliorated diabetic thoracic 
      aortic complications through the regulation of apoptosis, oxidative stress, and 
      inflammatory microenvironment mediating by the miR-223-3p/RBP-J/IRF8 axis.
CI  - Copyright (c) 2022 Ye Tian et al.
FAU - Tian, Ye
AU  - Tian Y
AUID- ORCID: 0000-0003-3773-3542
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Xu, Gang
AU  - Xu G
AUID- ORCID: 0000-0003-4455-3267
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Gao, Hong
AU  - Gao H
AUID- ORCID: 0000-0002-3050-6462
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Xie, Hong-Yan
AU  - Xie HY
AUID- ORCID: 0000-0002-0153-9986
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Leng, Yu-Lin
AU  - Leng YL
AUID- ORCID: 0000-0003-3408-7768
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Fu, Xiao-Xu
AU  - Fu XX
AUID- ORCID: 0000-0002-4148-4393
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Xie, Chun-Guang
AU  - Xie CG
AUID- ORCID: 0000-0001-9120-8605
AD  - TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20220928
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC9534610
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2022/10/11 06:00
MHDA- 2022/10/11 06:01
PMCR- 2022/09/28
CRDT- 2022/10/10 04:22
PHST- 2022/05/24 00:00 [received]
PHST- 2022/08/18 00:00 [revised]
PHST- 2022/08/29 00:00 [accepted]
PHST- 2022/10/10 04:22 [entrez]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2022/10/11 06:01 [medline]
PHST- 2022/09/28 00:00 [pmc-release]
AID - 10.1155/2022/6686931 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2022 Sep 28;2022:6686931. doi: 
      10.1155/2022/6686931. eCollection 2022.