PMID- 36213411
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221011
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips 
      from dead animals.
PG  - 993442
LID - 10.3389/fvets.2022.993442 [doi]
LID - 993442
AB  - The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges 
      on monitoring and surveillance. The objective of this study was to assess PRRSV 
      RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to 
      serum samples, processing fluids, and family oral fluids. Tongue tips and serum 
      samples were collected from three PRRSV-positive breeding herd farms (farms A, B, 
      and C) of three different age groups: newborns (<24 h), processing (2 to 7 days 
      of age), and weaning (18 to 22 days of age). Additionally, processing fluids and 
      family oral fluids were collected from 2-7 days of age and weaning age, 
      respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all 
      age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in 
      pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family 
      oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips 
      was numerically lower than the average Ct value from serum samples in the newborn 
      age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family 
      oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected 
      in tongue tips when pooled serum samples from the same age group tested PRRSV 
      RNA-negative. Taken together, these results demonstrate the potential value of 
      tongue tips for PRRSV monitoring and surveillance.
CI  - Copyright (c) 2022 Machado, Magalhaes, Poeta Silva, Moraes, Cezar, Mil-Homens, 
      Osemeke, Paiva, Moura, Gauger, Trevisan, Silva and Linhares.
FAU - Machado, Isadora F
AU  - Machado IF
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Magalhaes, Edison S
AU  - Magalhaes ES
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Poeta Silva, Ana Paula S
AU  - Poeta Silva APS
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Moraes, Daniel C A
AU  - Moraes DCA
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Cezar, Guilherme
AU  - Cezar G
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Mil-Homens, Mafalda P
AU  - Mil-Homens MP
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Osemeke, Onyekachukwu H
AU  - Osemeke OH
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Paiva, Rodrigo
AU  - Paiva R
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Moura, Cesar A A
AU  - Moura CAA
AD  - Iowa Select Farms, Iowa Falls, IA, United States.
FAU - Gauger, Phillip
AU  - Gauger P
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Trevisan, Giovani
AU  - Trevisan G
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Silva, Gustavo S
AU  - Silva GS
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
FAU - Linhares, Daniel C L
AU  - Linhares DCL
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, United States.
LA  - eng
PT  - Journal Article
DEP - 20220921
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9533096
OTO - NOTNLM
OT  - PRRSV
OT  - family oral fluids
OT  - pooled serum samples
OT  - population-based sampling
OT  - processing fluids
OT  - surveillance
OT  - tongue tips
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/10/11 06:00
MHDA- 2022/10/11 06:01
PMCR- 2022/01/01
CRDT- 2022/10/10 04:31
PHST- 2022/07/13 00:00 [received]
PHST- 2022/08/18 00:00 [accepted]
PHST- 2022/10/10 04:31 [entrez]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2022/10/11 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.993442 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Sep 21;9:993442. doi: 10.3389/fvets.2022.993442. eCollection 
      2022.