PMID- 36213652 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221011 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 9 DP - 2022 TI - Association between inflammatory cytokines in the aqueous humor and hyperreflective foci on optical coherence tomography in patients with neovascular age-related macular degeneration and polypoidal choroidal vasculopathy. PG - 973025 LID - 10.3389/fmed.2022.973025 [doi] LID - 973025 AB - PURPOSE: To investigate the associations between cytokine levels in the aqueous humor (AH) and hyperreflective foci (HF) on spectral-domain optical coherence tomography (SD-OCT) in neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV). METHODS: The prospective study included 63 eyes with nAMD, 44 with PCV, and 43 with cataracts (Controls). AH samples were obtained before anti-vascular endothelial growth factor (VEGF) therapy or cataract surgery. Cytokines interleukin 6 (IL-6), IL-8, IL-10, interferon-inducible protein 10 (IP-10), monocyte chemotactic protein 1 (MCP-1), and VEGF were measured by multiplex bead assay. Best-corrected visual acuity (BCVA), central macular thickness (CMT), and the number of HF were evaluated at baseline and 1 month after anti-VEGF treatment. RESULTS: No significances difference in IL-6 and IL-8 levels were noted among the three groups (P = 0.370 and P = 0.067). VEGF, IP-10, and IL-10 levels were significantly higher in nAMD and PCV groups than in Controls (all P < 0.05). In nAMD, HF was positively correlated with VEGF (r (s) = 0.300, P = 0.025) and in eyes with HF group, VEGF and IL-10 were significantly higher than those without HF (P = 0.008 and P = 0.022). In PCV, no correlation was observed between HF and cytokines (all P > 0.05). After anti-VEGF treatment, patients with HF in nAMD and PCV were predisposed to worse visual outcomes (P = 0.022 and P = 0.015) and a significantly greater reduction in CMT (P = 0.001 and P = 0.057). And nAMD patients with HF were more sensitive to anti-VEGF treatment than those without HF (P = 0.029). CONCLUSIONS: In the nAMD group, HF was positively correlated with VEGF. Patients in nAMD with HF had elevated levels of VEGF and IL-10 and responded favorably to anti-VEGF. HF might serve as an inflammatory biomarker and a predictive factor for therapeutic efficacy in patients with nAMD. CI - Copyright (c) 2022 Mao, Chen, Zhang, Fang, Zheng, Wu, Ye, Chen, Chen and Shen. FAU - Mao, Jianbo AU - Mao J AD - Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital, Hangzhou, China. AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Chen, Nuo AU - Chen N AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Zhang, Shian AU - Zhang S AD - Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital, Hangzhou, China. FAU - Fang, Yuyan AU - Fang Y AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Zheng, Zicheng AU - Zheng Z AD - Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, China. FAU - Wu, Sulan AU - Wu S AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Ye, Xin AU - Ye X AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Chen, Yijing AU - Chen Y AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Chen, Yiqi AU - Chen Y AD - Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital, Hangzhou, China. AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. FAU - Shen, Lijun AU - Shen L AD - Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital, Hangzhou, China. AD - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China. LA - eng PT - Journal Article DEP - 20220923 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC9538653 OTO - NOTNLM OT - aqueous humor OT - cytokine OT - hyperreflective foci OT - neovascular age-related macular degeneration OT - polypoidal choroidal vasculopathy OT - spectral-domain optical coherence tomography COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/10/11 06:00 MHDA- 2022/10/11 06:01 PMCR- 2022/09/23 CRDT- 2022/10/10 04:35 PHST- 2022/06/19 00:00 [received] PHST- 2022/08/31 00:00 [accepted] PHST- 2022/10/10 04:35 [entrez] PHST- 2022/10/11 06:00 [pubmed] PHST- 2022/10/11 06:01 [medline] PHST- 2022/09/23 00:00 [pmc-release] AID - 10.3389/fmed.2022.973025 [doi] PST - epublish SO - Front Med (Lausanne). 2022 Sep 23;9:973025. doi: 10.3389/fmed.2022.973025. eCollection 2022.