PMID- 36213788
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221011
IS  - 0912-0009 (Print)
IS  - 1880-5086 (Electronic)
IS  - 0912-0009 (Linking)
VI  - 71
IP  - 2
DP  - 2022 Sep
TI  - Carotenoid transporter CD36 expression depends on hypoxia-inducible factor-1alpha in 
      mouse soleus muscles.
PG  - 112-121
LID - 10.3164/jcbn.21-163 [doi]
AB  - Dietary beta-carotene induces muscle hypertrophy and prevents muscle atrophy in red 
      slow-twitch soleus muscles, but not in white fast-twitch extensor digitorum 
      longus (EDL) muscles and gastrocnemius muscles. However, it remains unclear why 
      these beneficial effects of beta-carotene are elicited in soleus muscles. To address 
      this issue, we focused on carotenoid transporters in skeletal muscles. In mice, 
      Cd36 mRNA levels were higher in red muscle than in white muscle. The 
      siRNA-mediated knockdown of CD36 decreased beta-carotene uptake in C2C12 myotubes. 
      In soleus muscles, CD36 knockdown inhibited beta-carotene-induced increase in muscle 
      mass. Intravenous injection of the hypoxia marker pimonidazole produced more 
      pimonidazole-bound proteins in soleus muscles than in EDL muscles, and the 
      hypoxia-inducible factor-1 (HIF-1) alpha protein level was higher in soleus muscles 
      than in EDL muscles. In C2C12 myotubes, hypoxia increased the expression of CD36 
      and HIF-1alpha at the protein and mRNA levels, and HIF-1alpha knockdown reduced 
      hypoxia-induced increase in Cd36 mRNA level. In soleus muscles, HIF-1alpha knockdown 
      reduced Cd36 mRNA level. These results indicate that CD36 is predominantly 
      involved in beta-carotene-induced increase in soleus muscle mass of mice. 
      Furthermore, we demonstrate that CD36 expression depends on HIF-1alpha in the soleus 
      muscles of mice, even under normal physiological conditions.
CI  - Copyright (c) 2022 JCBN.
FAU - Kitakaze, Tomoya
AU  - Kitakaze T
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
FAU - Sugihira, Takashi
AU  - Sugihira T
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
FAU - Kameyama, Hiromichi
AU  - Kameyama H
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
FAU - Maruchi, Asami
AU  - Maruchi A
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
FAU - Kobayashi, Yasuyuki
AU  - Kobayashi Y
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
FAU - Harada, Naoki
AU  - Harada N
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
FAU - Yamaji, Ryoichi
AU  - Yamaji R
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 
      599-8531, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220526
PL  - Japan
TA  - J Clin Biochem Nutr
JT  - Journal of clinical biochemistry and nutrition
JID - 8700907
PMC - PMC9519423
OTO - NOTNLM
OT  - CD36
OT  - carotenoid transporter
OT  - hypoxia-inducible factor-1alpha
OT  - soleus muscle
OT  - beta-carotene
COIS- No potential conflicts of interest were disclosed.
EDAT- 2022/10/11 06:00
MHDA- 2022/10/11 06:01
PMCR- 2022/09/01
CRDT- 2022/10/10 04:38
PHST- 2021/12/10 00:00 [received]
PHST- 2022/03/22 00:00 [accepted]
PHST- 2022/10/10 04:38 [entrez]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2022/10/11 06:01 [medline]
PHST- 2022/09/01 00:00 [pmc-release]
AID - DN/JST.JSTAGE/jcbn/21-163 [pii]
AID - 10.3164/jcbn.21-163 [doi]
PST - ppublish
SO  - J Clin Biochem Nutr. 2022 Sep;71(2):112-121. doi: 10.3164/jcbn.21-163. Epub 2022 
      May 26.