PMID- 36214215
OWN - NLM
STAT- MEDLINE
DCOM- 20230213
LR  - 20230213
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 37
IP  - 1
DP  - 2023 Jan
TI  - Examination of antimicrobial effect of fluoxetine in experimental sepsis model: 
      An in vivo study.
PG  - e23240
LID - 10.1002/jbt.23240 [doi]
AB  - Since most infectious diseases can develop into sepsis, it is still a major 
      medical problem. Some in-vivo studies showed promising properties of fluoxetine 
      in the treatment of infections. This study aims the antimicrobial effect of 
      fluoxetine on the inflammatory process used in the treatment of sepsis-modeled 
      rats. Besides, to investigate the efficacy of fluoxetine on modifying the 
      antibiotic effect of imipenem in the inflammatory response. An experimental 
      sepsis model was divided into negative control, positive control, fluoxetine 
      5 mg/kg, imipenem 60 mg/kg, and combined (fluoxetine; imipenem). Procalcitonin 
      (PCT), high-sensitivity C-reactive protein (hs-CRP), lactate, myeloperoxidase 
      activity (MPO), the inflammation markers interleukin-1beta (IL-1beta), interleukin-6 
      (IL-6), tumor necrosis factor-alfa (TNF-alpha), and monocyte chemoattractant 
      protein-1 (MCP-1) levels were measured by enzyme-linked immunosorbent assay 
      method. Oxidative stress markers, total oxidant status (TOS), total antioxidant 
      status (TAS), total thiol (TT), and native thiol (NT) were measured using 
      photometric methods. Oxidative stress index (OSI) was calculated according to TAS 
      and TOS levels. The statistical analysis was performed by Statistical Package for 
      Social Sciences version 22.0. After treatment with fluoxetine, imipenem, and 
      combined groups, IL-1beta, IL-6, TNF-alpha, MPO activity, MCP-1, hs-CRP, PCT, lactate, 
      and the oxidative stress markers OSI, and disulfide levels were decreased 
      (p < 0.05). The TT, NT, and TAS levels significantly statistically increased 
      (p < 0.05). This research demonstrates that fluoxetine has effects as 
      anti-inflammatory and antioxidant, and the combined treatment with antibioticum 
      imipenem indicates positive synergistic effects in the experimental sepsis model.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Cakir, Ahsen
AU  - Cakir A
AUID- ORCID: 0000-0002-2121-2198
AD  - Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
FAU - Bozali, Kubra
AU  - Bozali K
AUID- ORCID: 0000-0003-2416-0773
AD  - Faculty Life Sciences and Environmental Technology, Avans University of Applied 
      Science, Breda, The Netherlands.
AD  - Department of Medical Biochemistry, Faculty of Hamidiye Medicine, University of 
      Health Sciences Turkey, Istanbul, Turkey.
FAU - Celikten, Mert
AU  - Celikten M
AUID- ORCID: 0000-0002-8215-3638
AD  - Department of Scientific Research Projects, Experimental Application and Research 
      Center, Bezmialem Vakif University, Istanbul, Turkey.
AD  - Department of Anatomy, Medipol Institute of Health Sciences, Istanbul, Turkey.
FAU - Guler, Eray Metin
AU  - Guler EM
AUID- ORCID: 0000-0003-4351-1719
AD  - Department of Medical Biochemistry, Faculty of Hamidiye Medicine, University of 
      Health Sciences Turkey, Istanbul, Turkey.
AD  - Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif 
      University, Istanbul, Turkey.
FAU - Sahan, Ebru
AU  - Sahan E
AUID- ORCID: 0000-0002-1287-8166
AD  - Department of Psychiatry, Faculty of Medicine, Bezmialem Vakif University, 
      Istanbul, Turkey.
FAU - Durdu, Bulent
AU  - Durdu B
AUID- ORCID: 0000-0002-0244-4006
AD  - Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, 
      Bezmialem Vakif University, Istanbul, Turkey.
FAU - Sumbul, Bilge
AU  - Sumbul B
AUID- ORCID: 0000-0002-8768-3777
AD  - Department of Medical Microbiology, Faculty of Medicine, Bezmialem Vakif 
      University, Istanbul, Turkey.
FAU - Kocyigit, Abdurrahim
AU  - Kocyigit A
AUID- ORCID: 0000-0003-2335-412X
AD  - Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif 
      University, Istanbul, Turkey.
LA  - eng
GR  - E8.2019/3/Bezmialem Vakif Universitesi/
PT  - Journal Article
DEP - 20221010
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Antioxidants)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 71OTZ9ZE0A (Imipenem)
RN  - 0 (Interleukin-6)
RN  - 0 (Lactates)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Anti-Infective Agents/pharmacology
MH  - Antioxidants/pharmacology
MH  - C-Reactive Protein/metabolism
MH  - *Fluoxetine/pharmacology/therapeutic use
MH  - Imipenem/pharmacology/therapeutic use
MH  - Interleukin-6/metabolism
MH  - Lactates
MH  - Oxidative Stress
MH  - *Sepsis/drug therapy
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Disease Models, Animal
OTO - NOTNLM
OT  - antidepressive
OT  - fluoxetine
OT  - imipenem
OT  - inflammation
OT  - sepsis
EDAT- 2022/10/11 06:00
MHDA- 2023/01/18 06:00
CRDT- 2022/10/10 06:02
PHST- 2022/08/03 00:00 [revised]
PHST- 2022/02/26 00:00 [received]
PHST- 2022/09/22 00:00 [accepted]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2023/01/18 06:00 [medline]
PHST- 2022/10/10 06:02 [entrez]
AID - 10.1002/jbt.23240 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2023 Jan;37(1):e23240. doi: 10.1002/jbt.23240. Epub 2022 
      Oct 10.