PMID- 36214618 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221019 IS - 1415-4757 (Print) IS - 1678-4685 (Electronic) IS - 1415-4757 (Linking) VI - 45 IP - 3 DP - 2022 TI - Development and identification of an elite wheat-Hordeum californicum T6HcS/6BL translocation line ND646 containing several desirable traits. PG - e20220117 LID - S1415-47572022000300302 [pii] LID - 10.1590/1678-4685-GMB-2022-0117 [doi] LID - e20220117 AB - Hordeum californicum (H. californicum, 2n=2X=14, HcHc), one of the wild relatives of wheat (Triticum aestivum L.), harbors many desirable genes and is a potential genetic resource for wheat improvement. In this study, an elite line ND646 was selected from a BC4F5 population, which was developed using 60Co-gamma irradiated wheat-H. californicum disomic addition line WJ28-1 (DA6Hc) as the donor parent and Ningchun 4 as the recurrent parent. ND646 was identified as a novel wheat-H. californicum 6HcS/6BL translocation line using genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and H. californicum-specific expressed sequence tag (EST) markers. Further evaluation revealed that ND646 had excellent performance in several traits, such as a higher sedimentation value (SV), higher water absorption rate (WAR), and higher hardness index (HI). More importantly, it had more kernels per spike (KPS), a higher grain yields (GY), and good resistance to powdery mildew, leaf rust, and 2,4-D butylate (2,4-D). Its excellent phenotypic performance laid the foundation for further investigation of its genetic architecture and makes ND646 a useful germplasm resource for wheat breeding. FAU - Wang, Zhangjun AU - Wang Z AD - Nanjing Agricultural University, Cytogenetics Institute, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, Jiangsu, China. AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Li, Qingfeng AU - Li Q AUID- ORCID: 0000-0002-7639-7652 AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Liu, Caixia AU - Liu C AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Liu, Fenglou AU - Liu F AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Xu, Nali AU - Xu N AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Yao, Mingming AU - Yao M AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Yu, Huixia AU - Yu H AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Wang, Yanqing AU - Wang Y AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Chen, Jiajing AU - Chen J AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Bai, Shuangyu AU - Bai S AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Yang, Jingxin AU - Yang J AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Sun, Gang AU - Sun G AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Long, Jiaohui AU - Long J AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Fan, Yalei AU - Fan Y AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Kang, Ling AU - Kang L AD - Ningxia Academy of Agricultural-Forestry Sciences, Institute of Crop Sciences, Yinchuan, Ningxia, China. FAU - Li, Hongxia AU - Li H AD - Ningxia Academy of Agricultural-Forestry Sciences, Institute of Crop Sciences, Yinchuan, Ningxia, China. FAU - Zhang, Xiaogang AU - Zhang X AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. FAU - Liu, Shengxiang AU - Liu S AD - Ningxia University, School of Agriculture, Yinchuan, Ningxia, China. LA - eng GR - R01 EB004070/EB/NIBIB NIH HHS/United States PT - Journal Article DEP - 20221007 PL - Brazil TA - Genet Mol Biol JT - Genetics and molecular biology JID - 100883590 PMC - PMC9549530 COIS- Conflict of Interest: The authors declare no conflict of interest. EDAT- 2022/10/11 06:00 MHDA- 2022/10/11 06:01 PMCR- 2022/10/07 CRDT- 2022/10/10 09:32 PHST- 2022/03/24 00:00 [received] PHST- 2022/08/18 00:00 [accepted] PHST- 2022/10/10 09:32 [entrez] PHST- 2022/10/11 06:00 [pubmed] PHST- 2022/10/11 06:01 [medline] PHST- 2022/10/07 00:00 [pmc-release] AID - S1415-47572022000300302 [pii] AID - 10.1590/1678-4685-GMB-2022-0117 [doi] PST - epublish SO - Genet Mol Biol. 2022 Oct 7;45(3):e20220117. doi: 10.1590/1678-4685-GMB-2022-0117. eCollection 2022.