PMID- 36214976
OWN - NLM
STAT- MEDLINE
DCOM- 20230302
LR  - 20230302
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Linking)
VI  - 65
IP  - 4
DP  - 2023 Apr
TI  - Paired Box 5-Induced LINC00467 Upregulation Promotes the Progression of Laryngeal 
      Squamous Cell Cancer by Triggering the MicroRNA-4735-3p/TNF Alpha-Induced Protein 
      3 Pathway.
PG  - 655-667
LID - 10.1007/s12033-022-00564-3 [doi]
AB  - LINC00467 was reported as an oncogenic gene in different types of human cancers. 
      In this study, we investigated the molecular mechanisms of LINC00467 in the 
      tumorigenesis of laryngeal squamous cell cancer (LSCC). RT-qPCR was utilized to 
      detect the mRNA expression of genes, and western blot assay was used to determine 
      the protein levels of TNF alpha-induced protein 3 (TNFAIP3). The cell viability 
      was detected by CCK-8 assay. Transwell assays were conducted to determine the 
      cell migration and invasion of LSCC cells, and the cell cycle was assessed by 
      flow cytometry. The association between paired box 5 (PAX5), LINC00467, 
      miR-4735-3p, and TNFAIP3 was verified using ChIP, RNA pull-down, or luciferase 
      reporter assays. In our study, we found that LINC00467 was upregulated in LSCC, 
      and knockdown of LINC00467 suppressed cell viability and metastasis of LSCC. 
      Besides, LINC00467 transcription could be activated by PAX5 in LSCC. Furthermore, 
      LINC00467 acted as competitive endogenous RNA (ceRNA) for miR-4735-3p to 
      accelerate LSCC progression. In the meantime, TNFAIP3 was identified as a 
      downstream gene of miR-4735-3p. Finally, TNFAIP3 overexpression could overturn 
      the effects of miR-4735-3p mimic on LSCC cellular activities. In conclusion, our 
      results demonstrated that PAX5-induced LINC00467 facilitated LSCC progression by 
      inhibiting miR-4735-3p to increase TNFAIP3 expression.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China.
AD  - Department of Otorhinolaryngology, The Third People's Hospital of Deqing, Deqing, 
      China.
FAU - Wu, Yong
AU  - Wu Y
AD  - Department of Otorhinolaryngology, The First People's Hospital of Tongxiang, 
      Jiaxing, China.
FAU - Dai, Lili
AU  - Dai L
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China.
FAU - Wu, Honglin
AU  - Wu H
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China.
FAU - Chen, Chaohui
AU  - Chen C
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China.
FAU - Ni, Jiayu
AU  - Ni J
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China.
FAU - Jin, Enli
AU  - Jin E
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China.
FAU - Zhou, Xuehua
AU  - Zhou X
AUID- ORCID: 0000-0003-3259-3392
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, No. 126, Wenzhou Road, Hangzhou, 310015, Zhejiang 
      Province, China. xuehuazhou22@163.com.
LA  - eng
GR  - Y2100578/National Natural Science Foundation of Zhejiang province, China/
GR  - Y2090486/National Natural Science Foundation of Zhejiang province, China/
GR  - 2017RC011/Medical and health research project of Zhejiang Province/
GR  - 20110833B09/Development project of science and Technology of Hangzhou/
PT  - Journal Article
DEP - 20221010
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
EIN - Mol Biotechnol. 2022 Oct 31;:. PMID: 36315336
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - Up-Regulation
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - *Carcinoma, Squamous Cell/metabolism
MH  - *Laryngeal Neoplasms/genetics/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *Head and Neck Neoplasms/genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
OTO - NOTNLM
OT  - LINC00467
OT  - Laryngeal squamous cell cancer cells
OT  - PAX5
OT  - TNFAIP3
OT  - miR-4735-3p
EDAT- 2022/10/11 06:00
MHDA- 2023/03/03 06:00
CRDT- 2022/10/10 11:20
PHST- 2022/09/08 00:00 [accepted]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/10/10 11:20 [entrez]
AID - 10.1007/s12033-022-00564-3 [pii]
AID - 10.1007/s12033-022-00564-3 [doi]
PST - ppublish
SO  - Mol Biotechnol. 2023 Apr;65(4):655-667. doi: 10.1007/s12033-022-00564-3. Epub 
      2022 Oct 10.