PMID- 36215828
OWN - NLM
STAT- MEDLINE
DCOM- 20221128
LR  - 20221202
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 152
DP  - 2022 Dec
TI  - Ephedrae Herba polysaccharides inhibit the inflammation of ovalbumin induced 
      asthma by regulating Th1/Th2 and Th17/Treg cell immune imbalance.
PG  - 14-26
LID - S0161-5890(22)00431-X [pii]
LID - 10.1016/j.molimm.2022.09.009 [doi]
AB  - AIMS: This study aimed to investigate the anti-asthma effects of Ephedrae Herba 
      polysaccharides (PE) and possible mechanisms related to immune inflammatory 
      response. METHODS: An asthma model was established in rats using ovalbumin (OVA). 
      Seventy rats were randomly assigned to five groups: control, model, dexamethasone 
      (DEX, 0.075 mg/kg), low dose polysaccharides (LPE, 137.71 mg/kg) and high dose 
      polysaccharides (HPE, 275.42 mg/kg). The cough and asthma were used to evaluate 
      the basic state of asthmatic rats. Histological studies were evaluated by 
      hematoxylin and eosin (H&E), Masson, and periodic acid-schiff (PAS) staining. The 
      levels of interferon-gamma (IFN-gamma), interleukin (IL)-4, immunoglobulin E (IgE), tumor 
      necrosis factor alpha (TNF-alpha), and IL-17A in bronchoalveolar lavage fluid (BALF), and 
      the levels of transforming growth factor beta1 (TGF-beta1), IL-6, and IL-10 in serum 
      were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA levels of 
      Ifn-gamma, Il-4, Tgf-beta1, Il-6, Il-10, Tnf-alpha, Il-13, and Il-17a were evaluated by 
      quantitative real-time reverse transcription (qRT)-PCR. The dendritic cell (DCs), 
      T helper cell (Th), natural killer cell (NK), regulatory T cell (Treg), and Th17 
      cells in blood, the lymphocytes, macrophages and neutrophils in spleen, and cell 
      apoptosis and reactive oxygen species (ROS) in lung were analysed by flow 
      cytometry (FCM). Immunohistochemistry (IHC) was used to stain DCs (CD11c(+), 
      CD86(+), and CD80(+)), macrophages (CD68(+)), and neutrophils (MPO(+)) in the 
      spleen and lung. The protein levels of IL-17A, CD11c, CD86, and CD80 in lung were 
      measured by western blot. RESULTS: Our study demonstrated that PE could 
      effectively improve the symptoms of asthmatic rats, ameliorate the lung 
      pathological injury, inhibit inflammation, apoptosis and oxidative stress, 
      regulate the levels of macrophages, neutrophils, DCs, NK, Thc, Treg and Th17 
      cells. CONCLUSION: PE could collectively inhibit the inflammation, apoptosis and 
      ROS in asthma rats induced by OVA via regulating Th1/Th2 and Th17/Treg cell 
      immune imbalance.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Zhang, Beibei
AU  - Zhang B
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Zeng, Mengnan
AU  - Zeng M
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China; Collaborative 
      Innovation Center for Chinese Medicine and Respiratory Diseases co-constructed by 
      Henan province and Education Ministry of P.R. China, Henan University of Chinese 
      Medicine, Zhengzhou 450046, China.
FAU - Zhang, Qinqin
AU  - Zhang Q
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Wang, Ru
AU  - Wang R
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Jia, Jufang
AU  - Jia J
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Cao, Bing
AU  - Cao B
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Liu, Meng
AU  - Liu M
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Guo, Pengli
AU  - Guo P
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Zhang, Yuhan
AU  - Zhang Y
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China.
FAU - Zheng, Xiaoke
AU  - Zheng X
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China; Collaborative 
      Innovation Center for Chinese Medicine and Respiratory Diseases co-constructed by 
      Henan province and Education Ministry of P.R. China, Henan University of Chinese 
      Medicine, Zhengzhou 450046, China. Electronic address: zhengxk.2006@163.com.
FAU - Feng, Weisheng
AU  - Feng W
AD  - Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, 
      China; The Engineering and Technology Center for Chinese Medicine Development of 
      Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China; Collaborative 
      Innovation Center for Chinese Medicine and Respiratory Diseases co-constructed by 
      Henan province and Education Ministry of P.R. China, Henan University of Chinese 
      Medicine, Zhengzhou 450046, China. Electronic address: fwsh@hactcm.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221007
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 130068-27-8 (Interleukin-10)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-6)
RN  - 0 (Polysaccharides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Phytochemicals)
RN  - 51QBA3IQ91 (Ephedrae herba)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Rats
MH  - *Asthma/chemically induced/drug therapy
MH  - Bronchoalveolar Lavage Fluid
MH  - Disease Models, Animal
MH  - Inflammation
MH  - Interleukin-10/metabolism
MH  - Interleukin-17/metabolism
MH  - Interleukin-6/metabolism
MH  - Lung/pathology
MH  - Mice, Inbred BALB C
MH  - *Polysaccharides/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - *T-Lymphocytes, Regulatory
MH  - Th17 Cells
MH  - Transforming Growth Factor beta1/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Phytochemicals/pharmacology
MH  - *Drugs, Chinese Herbal/pharmacology
OTO - NOTNLM
OT  - Allergic immune response
OT  - Asthma
OT  - Ephedrae Herba polysaccharides
OT  - Ovalbumin-induced airway inflammation
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2022/10/11 06:00
MHDA- 2022/11/26 06:00
CRDT- 2022/10/10 18:23
PHST- 2022/06/01 00:00 [received]
PHST- 2022/09/18 00:00 [revised]
PHST- 2022/09/19 00:00 [accepted]
PHST- 2022/10/11 06:00 [pubmed]
PHST- 2022/11/26 06:00 [medline]
PHST- 2022/10/10 18:23 [entrez]
AID - S0161-5890(22)00431-X [pii]
AID - 10.1016/j.molimm.2022.09.009 [doi]
PST - ppublish
SO  - Mol Immunol. 2022 Dec;152:14-26. doi: 10.1016/j.molimm.2022.09.009. Epub 2022 Oct 
      7.