PMID- 36219154
OWN - NLM
STAT- MEDLINE
DCOM- 20230329
LR  - 20230406
IS  - 1568-5624 (Electronic)
IS  - 0920-5063 (Linking)
VI  - 34
IP  - 5
DP  - 2023 Apr
TI  - Preparation of hybrid meniscal constructs using hydrogels and acellular matrices.
PG  - 587-611
LID - 10.1080/09205063.2022.2135078 [doi]
AB  - To search for a suitable meniscus repair material, acellular hybrid scaffolds 
      consisting of in situ cross-linkable 3-D interpenetrating network structures were 
      obtained by decellularization of the meniscus tissues followed by integration of 
      the gel system. Decellularization efficiency was confirmed using a DNA 
      quantification assay (82% decrease in DNA content) and histological stainings. In 
      the second part of the study, the gelatin molecule was functionalized by adding 
      methacrylic anhydride and the degree of functionalization was found to be 75% by 
      (Proton-Nuclear Magnetic Resonance) (1)H-NMR. Using this, a series of hybrid 
      constructs named GelMA-Hybrid (G-Hybrid), GELMA/PEGDMA-Hybrid (PG-Hybrid), and 
      GelMA/PEGDMA/HAMA-Hybrid (PGH-Hybrid) were prepared by cross-linking with UVA. 
      Changes in the chemical structure were determined with Fourier Transform Infrared 
      Spectrophotometer (FTIR). Water uptake capacities of cross-linked hybrid 
      structures were measured in swelling studies, and it was found that hybrid 
      scaffolds showed similar swelling properties compared to native counterparts. By 
      compressive mechanical tests, enhanced mechanical properties were revealed in 
      cross-linked scaffolds with PGH-Hybrid having the highest cross-link density. 
      Protein denaturation and decomposition transition temperatures were improved by 
      adding hydrogels to acellular scaffolds according to thermal gravimetric analyses 
      (TGA). Cross-linked acellular scaffolds have exhibited a behavior close to native 
      tissues with below 25% mass loss in phosphate buffer saline (PBS) and enzymatic 
      solution. Cell viability was examined through Alamar Blue on the first day and 
      cell viability in hybrid constructs was found to be above 80% while it was closer 
      to the control group on the 7(th) day. It was concluded that the developed 
      biomaterials could be used in meniscus tissue engineering with their tunable 
      physicochemical and mechanical properties.
FAU - Zihna, Gizem
AU  - Zihna G
AD  - Bioengineering Division, Institute of Science, Hacettepe University, Ankara, 
      Turkey.
FAU - Topuz, Bengisu
AU  - Topuz B
AD  - Bioengineering Division, Institute of Science, Hacettepe University, Ankara, 
      Turkey.
FAU - Gunal, Gulcin
AU  - Gunal G
AD  - Bioengineering Division, Institute of Science, Hacettepe University, Ankara, 
      Turkey.
FAU - Aydin, Halil Murat
AU  - Aydin HM
AUID- ORCID: 0000-0003-4107-4324
AD  - Bioengineering Division, Institute of Science, Hacettepe University, Ankara, 
      Turkey.
AD  - Centre for Bioengineering, Hacettepe University, Ankara, Turkey.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221022
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (poly(ethylene glycol)-dimethacrylate)
RN  - 0 (Hydrogels)
RN  - 9007-49-2 (DNA)
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - *Tissue Scaffolds/chemistry
MH  - Hydrogels/chemistry
MH  - Tissue Engineering
MH  - *Meniscus
MH  - DNA
MH  - Gelatin/chemistry
OTO - NOTNLM
OT  - Meniscus tissue engineering
OT  - decellularization
OT  - hybrid structures
OT  - hydrogels
OT  - photopolymerization
EDAT- 2022/10/12 06:00
MHDA- 2023/03/29 06:05
CRDT- 2022/10/11 10:33
PHST- 2023/03/29 06:05 [medline]
PHST- 2022/10/12 06:00 [pubmed]
PHST- 2022/10/11 10:33 [entrez]
AID - 10.1080/09205063.2022.2135078 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 2023 Apr;34(5):587-611. doi: 
      10.1080/09205063.2022.2135078. Epub 2022 Oct 22.