PMID- 36219533
OWN - NLM
STAT- MEDLINE
DCOM- 20230103
LR  - 20230707
IS  - 1756-185X (Electronic)
IS  - 1756-1841 (Linking)
VI  - 26
IP  - 1
DP  - 2023 Jan
TI  - Screening of key biomarkers in osteoporosis: Evidence from bioinformatic 
      analysis.
PG  - 69-79
LID - 10.1111/1756-185X.14450 [doi]
AB  - OBJECTIVE: To analyze the expression characteristics of osteoporosis-related 
      genes by bioinformatics and elucidate the pathogenesis of osteoporosis. METHODS: 
      The differentially expressed genes (DEGs), microRNA (miRNA), and genes with 
      differentially methylated regions (DMRS) in promoters were identified. The 
      protein-protein interaction (PPI) network was constructed and performed. The Clue 
      Gene Ontology analysis and miRNA-mRNA (messenger RNA) regulatory network were 
      constructed using Cytoscape. RESULTS: Fifty-nine DEGs, 10 differential miRNAs, 
      and 2083 genes with DMRs were screened out. The Proteasome-Modulator (PSMD) 
      family proteins and estrogen receptor 1 (ESR1) are vital for the PPI analysis of 
      DEGs. The interaction network of the Smad3 protein showed that the degree of 
      connection to ESR1, PSMD11, and transcription factor 4 (TCF4) is very high. Homo 
      sapiens (hsa)-miR-106b-5p was differential and regulated TCF4 through building 
      the miRNA-mRNA regulatory network. Kyoto Encyclopedia of Genes and Genomes (KEGG) 
      enrichment of DEGs focused on vascular smooth muscle contraction, thyroid hormone 
      signaling pathway, and estrogen signaling pathway. The Gene Ontology (GO) 
      function analysis of genes with DMRs in promoters was primarily concentrated in 
      the cell differentiation, positive regulation of CDP-diacylglycerol-serine 
      O-phosphatidyltransferase activity, and positive regulation of 
      C-palmitoyltransferase activity. The KEGG enrichment of genes with DMRs in 
      promoters largely focused on glycerol phospholipid metabolism, histidine 
      metabolism, Adenosine 5'-monophosphate-activated protein kinase signaling 
      pathway, Hedgehog signaling pathway, and mRNA surveillance pathway. CONCLUSION: 
      Hsa-miRNA-106b-5p regulates bone formation and the pathogenesis of osteoporosis 
      by controlling TCF4, and methylation modification of TCF4 can also affect the 
      pathogenesis of osteoporosis.
CI  - (c) 2022 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons 
      Australia, Ltd.
FAU - Chen, Hao
AU  - Chen H
AD  - Traditional Chinese Medicine Orthopedics, Nanjing University of Chinese Medicine, 
      Nanjing, China.
AD  - Department of Orthopedics and Traumatology, Yancheng Dafeng Hospital of 
      Traditional Chinese Medicine, Yancheng, China.
FAU - Li, Shaoshuo
AU  - Li S
AD  - Traditional Chinese Medicine Orthopedics, Nanjing University of Chinese Medicine, 
      Nanjing, China.
FAU - Wang, Jianwei
AU  - Wang J
AD  - Department of Orthopedics, Wuxi TCM Hospital Affiliated to Nanjing University of 
      Chinese Medicine, Wuxi, China.
FAU - Ma, Yong
AU  - Ma Y
AD  - Traditional Chinese Medicine Orthopedics, Nanjing University of Chinese Medicine, 
      Nanjing, China.
FAU - Yin, Heng
AU  - Yin H
AUID- ORCID: 0000-0001-9081-1082
AD  - Department of Orthopedics, Wuxi TCM Hospital Affiliated to Nanjing University of 
      Chinese Medicine, Wuxi, China.
LA  - eng
GR  - BJ2020063/Top Talent Support Program for young and middle-aged people of Wuxi 
      Health Committee/
GR  - BK20180167/Jiangsu Natural Science Foundation/
GR  - 81973878/National Natural Science Foundation of China/
GR  - 81873320/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221011
PL  - England
TA  - Int J Rheum Dis
JT  - International journal of rheumatic diseases
JID - 101474930
RN  - 0 (Hedgehog Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Biomarkers)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Int J Rheum Dis. 2023 Jul;26(7):1220-1221. PMID: 37394890
MH  - Humans
MH  - Gene Regulatory Networks
MH  - Gene Expression Profiling
MH  - Hedgehog Proteins
MH  - *MicroRNAs/genetics/metabolism
MH  - Biomarkers
MH  - Computational Biology
MH  - *Osteoporosis/diagnosis/genetics
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - DNA methylation
OT  - Smad3
OT  - bioinformatics
OT  - mRNA
OT  - microRNA
OT  - osteoporosis
EDAT- 2022/10/12 06:00
MHDA- 2023/01/04 06:00
CRDT- 2022/10/11 12:14
PHST- 2022/09/02 00:00 [revised]
PHST- 2022/06/07 00:00 [received]
PHST- 2022/09/20 00:00 [accepted]
PHST- 2022/10/12 06:00 [pubmed]
PHST- 2023/01/04 06:00 [medline]
PHST- 2022/10/11 12:14 [entrez]
AID - 10.1111/1756-185X.14450 [doi]
PST - ppublish
SO  - Int J Rheum Dis. 2023 Jan;26(1):69-79. doi: 10.1111/1756-185X.14450. Epub 2022 
      Oct 11.