PMID- 36219760
OWN - NLM
STAT- MEDLINE
DCOM- 20230105
LR  - 20230113
IS  - 1745-4514 (Electronic)
IS  - 0145-8884 (Linking)
VI  - 46
IP  - 12
DP  - 2022 Dec
TI  - Synergistic properties of garlic and Citrullus colocynthis on reproductive injury 
      caused by diabetes in male rats: Structural and molecular evidence.
PG  - e14467
LID - 10.1111/jfbc.14467 [doi]
AB  - This study evaluates the synergistic effect of garlic and Citrullus colocynthis 
      on diabetic reproductive damage by suppressing the AGEs/RAGE/Nox-4 signaling 
      pathway. Thirty-five male Wistar rats were divided into five groups 
      (n = 7/group): Control, Diabetic, Diabetic+G (Garlic, 1 mL/100 g b.w), Diabetic+C 
      (C. colocynthis, 10 mg/kg b.w) and Diabetic+GC (Garlic, 1 mL/100 g b.w and C. 
      colocynthis, 10 mg/kg b.w) groups. At the end of the experimental period 
      (30 days), in diabetic rats, glucose increased, and body & testis weight, 
      luteinizing hormone (LH) and testosterone levels, and sperm count decreased 
      significantly and histopathological injuries were observed. In addition, they 
      have increased testicular apoptosis and oxidative stress. Also, the mechanism 
      based on advanced glycation end products (AGEs)/receptors for advanced glycation 
      end products (RAGE)/NADPH oxidase-4 (Nox-4) was activated in diabetic rats. 
      Separate consumption of garlic and C. colocynthis in Diabetic+G and Diabetic+C 
      groups alleviated the negative adverse effect of diabetes to some extent, but 
      when they were used in the combination form (Diabetic+GC) improvement was 
      profound. Testis histopathology, increased body and testis weight, and enhanced 
      capacity in protecting diabetic reproductive injury was seen. Decreases in 
      testosterone and LH concentration and sperm count in diabetic rats were also 
      reversed by combined administration of garlic and C. colocynthis. It regulated 
      oxidative stress markers, meanwhile reducing caspase-3 immunoexpression. In 
      addition, overexpression of RAGE, Nox-4 and nuclear transcription factor-kappaB 
      (NF-kappaB) was inhibited by the combination of garlic and C. colocynthis. PRACTICAL 
      APPLICATIONS: Diabetes mellitus is wide spread all around the world with variety 
      of complications in body including reproductive system in which patients suffer 
      from physical and psychological aspects. Despite many efforts in providing agents 
      for controlling diabetes and its complications, economic conditions of some 
      countries make it difficult for people to provide costly medicine and as a 
      result, they have to bear the complications until they pass away. However, 
      traditional medicine is still finding its way, especially in poor countries with 
      emphasis on medicinal plants. There have been many studies on plants to alleviate 
      diabetes or its side effects. But, using one plant for long term, may be not so 
      effective. Here, we attempted to find whether two plants from two different 
      species can show more efficacy than each one alone. We noticed garlic and 
      Citrullus colocynthis despite having beneficial effects when used alone, they 
      could show synergistic effects in combination.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Mohammadzadeh, Aghileh
AU  - Mohammadzadeh A
AD  - Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, 
      Kerman, Iran.
FAU - Gol, Ali
AU  - Gol A
AD  - Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, 
      Kerman, Iran.
LA  - eng
PT  - Journal Article
DEP - 20221011
PL  - United States
TA  - J Food Biochem
JT  - Journal of food biochemistry
JID - 7706045
RN  - 0 (Antioxidants)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 3XMK78S47O (Testosterone)
RN  - 0 (Glycation End Products, Advanced)
SB  - IM
MH  - Rats
MH  - Male
MH  - Animals
MH  - *Citrullus colocynthis/metabolism
MH  - *Garlic/metabolism
MH  - *Diabetes Mellitus, Experimental/drug therapy/complications
MH  - Rats, Wistar
MH  - Seeds/metabolism
MH  - Antioxidants/pharmacology
MH  - Receptor for Advanced Glycation End Products/metabolism/therapeutic use
MH  - Testosterone
MH  - Glycation End Products, Advanced/metabolism/therapeutic use
OTO - NOTNLM
OT  - AGEs/RAGE
OT  - Nox-4
OT  - diabetes mellitus
OT  - medicinal plant
OT  - oxidative stress
EDAT- 2022/10/12 06:00
MHDA- 2023/01/06 06:00
CRDT- 2022/10/11 15:04
PHST- 2022/09/20 00:00 [revised]
PHST- 2022/07/22 00:00 [received]
PHST- 2022/09/24 00:00 [accepted]
PHST- 2022/10/12 06:00 [pubmed]
PHST- 2023/01/06 06:00 [medline]
PHST- 2022/10/11 15:04 [entrez]
AID - 10.1111/jfbc.14467 [doi]
PST - ppublish
SO  - J Food Biochem. 2022 Dec;46(12):e14467. doi: 10.1111/jfbc.14467. Epub 2022 Oct 
      11.