PMID- 36221092
OWN - NLM
STAT- MEDLINE
DCOM- 20221013
LR  - 20221015
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 18
IP  - 1
DP  - 2022 Oct 11
TI  - Establishment of a recombinase polymerase amplification (RPA) fluorescence assay 
      for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV).
PG  - 369
LID - 10.1186/s12917-022-03465-4 [doi]
LID - 369
AB  - BACKGROUND: Swine acute diarrhea syndrome coronavirus (SADS-CoV) causes acute 
      vomiting and diarrhea in piglets, leading to significant financial losses for the 
      pig industry. Recombinase polymerase amplification (RPA) is a rapid nucleic acid 
      amplification technology used under constant temperature conditions. The study 
      established a real-time reverse transcription (RT)-RPA assay for early diagnosis 
      of SADS-CoV.  RESULTS: The detection limit of the real-time RT-RPA was 74 
      copies/microL of SADS-CoV genomic standard recombinant plasmid in 95% of cases. The 
      assay was performed in less than 30 min and no cross-reactions were observed with 
      eight other common viruses that affect swine, including classical swine fever 
      virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudo 
      rabies virus (PRV), swine influenza virus (SIV), seneca valley virus (SVA), 
      transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus 
      (PEDV) and porcine deltacoronavirus (PDCoV). The coefficient of variation (C.V.) 
      values of the two standards dilutions and three positive clinical sample ranged 
      from 2.95% to 4.71%. A total of 72 clinical fecal samples from swine with 
      diarrheal symptoms were analyzed with the developed RT-RPA and quantitative 
      RT-PCR. There was 98.61% agreement between the RT-RPA and the quantitative 
      real-time PCR results. CONCLUSIONS: These results indicated that the developed 
      RT-RPA assay had good specificity, sensitivity, stability and repeatability. The 
      study successfully established a broadly reactive RT-RPA assay for SADS-CoV 
      detection.
CI  - (c) 2022. The Author(s).
FAU - Cong, Xiao
AU  - Cong X
AD  - College of Animal Science, Anhui Science and Technology University and Anhui 
      Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang, 
      China.
FAU - Zhu, Yujun
AU  - Zhu Y
AD  - Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key 
      Laboratory of Laboratory Animals, Guangzhou, China.
FAU - Liu, Xinchao
AU  - Liu X
AD  - College of Animal Science, Anhui Science and Technology University and Anhui 
      Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang, 
      China.
FAU - Lian, Yuexiao
AU  - Lian Y
AD  - Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key 
      Laboratory of Laboratory Animals, Guangzhou, China.
FAU - Huang, Bihong
AU  - Huang B
AD  - Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key 
      Laboratory of Laboratory Animals, Guangzhou, China.
FAU - Luo, Yinzhu
AU  - Luo Y
AD  - Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key 
      Laboratory of Laboratory Animals, Guangzhou, China.
FAU - Gu, Youfang
AU  - Gu Y
AD  - College of Animal Science, Anhui Science and Technology University and Anhui 
      Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang, 
      China. youfanggu@163.com.
FAU - Wu, Miaoli
AU  - Wu M
AD  - Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key 
      Laboratory of Laboratory Animals, Guangzhou, China. miaoli200607@163.com.
FAU - Shi, Yue
AU  - Shi Y
AD  - Beijing Biaochizehui Biotechnology Company Limited Daxing District, Qingfengxilu 
      No.29, Beijing, China. 106424013@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20221011
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Nucleic Acids)
RN  - 0 (Recombinases)
RN  - Swine acute diarrhea syndrome coronavirus
SB  - IM
MH  - *Alphacoronavirus/genetics
MH  - Animals
MH  - *Coronavirus Infections/diagnosis/veterinary
MH  - Diarrhea/diagnosis/veterinary
MH  - *Nucleic Acids
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Recombinases
MH  - Sensitivity and Specificity
MH  - Swine
MH  - *Swine Diseases/diagnosis
PMC - PMC9552127
OTO - NOTNLM
OT  - Point-of-care testing (POCT)
OT  - Recombinase polymerase amplification (RPA)
OT  - Repeatability
OT  - Sensitivity
OT  - Specificity
OT  - Stability
OT  - Swine acute diarrhea syndrome coronavirus (SADS-CoV)
COIS- The authors declare that they have no competing interests.
EDAT- 2022/10/12 06:00
MHDA- 2022/10/14 06:00
PMCR- 2022/10/11
CRDT- 2022/10/11 23:37
PHST- 2022/02/22 00:00 [received]
PHST- 2022/09/30 00:00 [accepted]
PHST- 2022/10/11 23:37 [entrez]
PHST- 2022/10/12 06:00 [pubmed]
PHST- 2022/10/14 06:00 [medline]
PHST- 2022/10/11 00:00 [pmc-release]
AID - 10.1186/s12917-022-03465-4 [pii]
AID - 3465 [pii]
AID - 10.1186/s12917-022-03465-4 [doi]
PST - epublish
SO  - BMC Vet Res. 2022 Oct 11;18(1):369. doi: 10.1186/s12917-022-03465-4.