PMID- 36224608
OWN - NLM
STAT- MEDLINE
DCOM- 20221014
LR  - 20221016
IS  - 1756-3305 (Electronic)
IS  - 1756-3305 (Linking)
VI  - 15
IP  - 1
DP  - 2022 Oct 12
TI  - Epitope vaccine design for Toxoplasma gondii based on a genome-wide database of 
      membrane proteins.
PG  - 364
LID - 10.1186/s13071-022-05497-z [doi]
LID - 364
AB  - BACKGROUND: There is presently no effective and safe vaccine for Toxoplasma 
      gondii for humans. The study described here was designed to search for a novel 
      group of optimal B cell and T cell epitopes from Toxoplasma membrane proteins 
      using genome-wide comprehensive screening. METHODS: The amino acid sequences of 
      membrane proteins of T. gondii were obtained from the UniProt database. The 
      ABCPred and BepiPred servers were employed to predict the linear B cell epitopes. 
      The Immune Epitope Database (IEDB) online service was utilized to forecast T cell 
      epitopes within T. gondii membrane proteins that bind to human leukocyte antigen 
      (HLA) class I (HLA-I) or HLA-II molecules. RESULTS: From the 314 membrane 
      proteins of T. gondii, a total of 14 linear B cell epitopes embedded in 12 
      membrane proteins were identified. Eight epitopes for major histocompatibility 
      complex (MHC) class I (MHC-I) molecules and 18 epitopes for MHC-II molecules were 
      ultimately selected, for which world population coverage percentiles were 71.94% 
      and 99.76%, respectively. The top rated combinations of linear B cell epitopes 
      and T cell epitopes covering both BALB/c mice and a majority of the human 
      population were identified for the development of a protective vaccine. 
      CONCLUSIONS: The ultimate vaccine construct described here, which comprises B 
      cells, MHC-I and MHC-II epitopes, might protect individuals against T. gondii 
      infection by inducing humoral and cellular immune responses.
CI  - (c) 2022. The Author(s).
FAU - Li, Xuan-Wu
AU  - Li XW
AD  - National Clinical Research Center for Metabolic Disease, Key Laboratory of 
      Diabetes Immunology, Ministry of Education, and Department of Metabolism and 
      Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, 
      China.
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Zhang, Ni
AU  - Zhang N
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Li, Zhuo-Lin
AU  - Li ZL
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Dibo, Nouhoum
AU  - Dibo N
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Ma, Zhen-Rong
AU  - Ma ZR
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Lu, Bin
AU  - Lu B
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Huang, Ye-Hong
AU  - Huang YH
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Chang, Yun-Feng
AU  - Chang YF
AD  - Department of Forensic Medicine Science, Xiangya School of Basic Medicine, 
      Central South University, Changsha, China.
FAU - Chen, Hong-Zhi
AU  - Chen HZ
AD  - National Clinical Research Center for Metabolic Disease, Key Laboratory of 
      Diabetes Immunology, Ministry of Education, and Department of Metabolism and 
      Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, 
      China. chenhongzhi2013@csu.edu.cn.
FAU - Wu, Xiang
AU  - Wu X
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China. wuxiang@csu.edu.cn.
AD  - Hunan Provincial Key Lab of Immunology and Transmission Control on 
      Schistosomiasis, Changsha, China. wuxiang@csu.edu.cn.
LA  - eng
GR  - 2018YFE0114500/National Key Research and Development Project/
GR  - 82072306/National Natural Sciences Foundation of China/
GR  - 82072306/National Natural Sciences Foundation of China/
GR  - 81970746/National Natural Sciences Foundation of China/
GR  - 2022JJ30797/Hunan Provincial Natural Sciences Foundation/
PT  - Journal Article
DEP - 20221012
PL  - England
TA  - Parasit Vectors
JT  - Parasites & vectors
JID - 101462774
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Membrane Proteins)
RN  - 0 (Vaccines)
SB  - IM
MH  - Animals
MH  - Epitopes, B-Lymphocyte/genetics
MH  - Epitopes, T-Lymphocyte/genetics
MH  - Histocompatibility Antigens Class II
MH  - Humans
MH  - Membrane Proteins/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Toxoplasma/genetics
MH  - *Vaccines
PMC - PMC9555269
OTO - NOTNLM
OT  - Bioinformatics
OT  - Epitope vaccine
OT  - Human leukocyte antigen
OT  - Toxoplasma gondii
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/10/13 06:00
MHDA- 2022/10/15 06:00
PMCR- 2022/10/12
CRDT- 2022/10/12 23:45
PHST- 2022/08/06 00:00 [received]
PHST- 2022/09/16 00:00 [accepted]
PHST- 2022/10/12 23:45 [entrez]
PHST- 2022/10/13 06:00 [pubmed]
PHST- 2022/10/15 06:00 [medline]
PHST- 2022/10/12 00:00 [pmc-release]
AID - 10.1186/s13071-022-05497-z [pii]
AID - 5497 [pii]
AID - 10.1186/s13071-022-05497-z [doi]
PST - epublish
SO  - Parasit Vectors. 2022 Oct 12;15(1):364. doi: 10.1186/s13071-022-05497-z.