PMID- 36229953
OWN - NLM
STAT- MEDLINE
DCOM- 20230213
LR  - 20230213
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 37
IP  - 2
DP  - 2023 Feb
TI  - Proanthocyanidins suppress NLRP3 inflammasome and M1 macrophage polarization to 
      alleviate severe acute pancreatitis in mice.
PG  - e23242
LID - 10.1002/jbt.23242 [doi]
AB  - The role of reactive oxygen species (ROS) is crucial for the pathogenesis of 
      acute pancreatitis (AP). Proanthocyanidins (PAs) have been confirmed to exert 
      antioxidant activity. Our study aimed to determine whether PAs alleviated SAP via 
      reducing ROS, suppressing NLRP3 inflammasome, and inhibiting M1 macrophage 
      polarization. Our study investigated the protective effects of PAs on pancreatic 
      histopathological injury using SAP mice. The effects of PAs on macrophages were 
      investigated in inflammatory RAW 264.7 cells or mouse bone marrow-derived 
      macrophages (BMDMs) induced by lipopolysaccharide (LPS). Immunofluorescence 
      staining and/or western blot assay were employed to evaluate NLRP3 inflammasome 
      in macrophages and pancreatic tissue. Cell counting kit-8 (CCK-8) was used to 
      access effects of PAs on cell viability and cytometry flow was used to determine 
      the effects of the PAs on the ROS levels of the RAW 264.7 cells. Then, we 
      evaluated M1 macrophage polarization using flow cytometry or real-time 
      quantitative polymerase chain reaction (RT-qPCR). PAs administration alleviated 
      pancreatic inflammation in SAP mice. The PAs depressed NLRP3 inflammasome and 
      inhibited M1 macrophage polarization in pancreatic tissue. We also found that the 
      PAs showed no cellular toxicity but decreased ROS levels in RAW 264.7 cells, 
      downregulated the NLRP3 inflammasome in the macrophages, and inhibited cell M1 
      macrophage polarization. Our study indicates the anti-inflammatory properties of 
      the PAs on SAP mice by decreasing ROS levels, suppressing NLRP3 inflammasome, and 
      M1 macrophage polarization.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Sheng, Li-Ping
AU  - Sheng LP
AUID- ORCID: 0000-0002-6490-3845
AD  - Department of Gastroenterology, The First Affiliated Hospital of Xiamen 
      University, School of Medicine, Xiamen University, Xiamen, China.
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Han, Chao-Qun
AU  - Han CQ
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Ling, Xin
AU  - Ling X
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Guo, Xian-Wen
AU  - Guo XW
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Lin, Rong
AU  - Lin R
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Ding, Zhen
AU  - Ding Z
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
AD  - Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, China.
LA  - eng
GR  - 81770637/National Natural Science Foundation of China/
GR  - 82070667/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221013
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Proanthocyanidins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Nlrp3 protein, mouse)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Inflammasomes
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - *Proanthocyanidins/pharmacology
MH  - *Pancreatitis/chemically induced/drug therapy
MH  - Reactive Oxygen Species
MH  - Acute Disease
MH  - Macrophages
OTO - NOTNLM
OT  - M1 macrophage polarization
OT  - NLRP3 inflammasome
OT  - proanthocyanidins
OT  - reactive oxygen species
OT  - severe acute pancreatitis
EDAT- 2022/10/15 06:00
MHDA- 2023/02/14 06:00
CRDT- 2022/10/14 01:23
PHST- 2022/07/28 00:00 [revised]
PHST- 2021/12/19 00:00 [received]
PHST- 2022/09/22 00:00 [accepted]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2023/02/14 06:00 [medline]
PHST- 2022/10/14 01:23 [entrez]
AID - 10.1002/jbt.23242 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2023 Feb;37(2):e23242. doi: 10.1002/jbt.23242. Epub 2022 
      Oct 13.