PMID- 36230453
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221017
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 12
IP  - 19
DP  - 2022 Oct 9
TI  - Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using 
      Reverse Transcription Loop-Mediated Isothermal Amplification Method.
LID - 10.3390/ani12192712 [doi]
LID - 2712
AB  - Porcine epidemic diarrhea virus (PEDV) can cause severe infectious porcine 
      epidemic diarrhea (PED) and infect different ages of pigs, resulting in sickness 
      and death among suckling pigs. For PEDV detection, finding an effective and rapid 
      method is a priority. In this study, we established an effective reverse 
      transcription loop-mediated isothermal amplification (RT-LAMP) method for PEDV 
      detection. Three sets of primers, specific for eight different sequences of the 
      PEDV N gene, were designed in this study. The optimized RT-LAMP amplification 
      program was as follows: 59 min at 61.9  degrees C and 3 min at 80  degrees C. The RT-LAMP results 
      were confirmed with the addition of SYBR Green I fluorescence dye and with the 
      detection of a ladder-like band by conventional gel electrophoresis analysis, 
      which demonstrated a significant agreement between the two methods. The LOD of 
      PEDV by RT-LAMP was 0.0001 ng/muL. Compared with RT-LAMP, the traditional RT-PCR 
      method is 100-fold less sensitive. The RT-LAMP results had no cross-reaction with 
      porcine parvovirus (PPV), porcine circovirus type 1 (PCV1), porcine pseudorabies 
      virus (PRV), porcine circovirus type 2 (PCV2), rotavirus (RV), transmissible 
      gastroenteritis virus (TGEV) and porcine reproductive and respiratory syndrome 
      virus (PRRSV). Consequently, the newly developed RT-LAMP method could provide an 
      accurate and reliable tool for PEDV diagnosis.
FAU - Li, Chunhua
AU  - Li C
AUID- ORCID: 0000-0002-7522-2279
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences, Shanghai 201106, China.
AD  - Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of 
      Agricultural Sciences, Shanghai 201106, China.
FAU - Liang, Jieling
AU  - Liang J
AD  - School of Life Science, Taizhou University, Taizhou 318000, China.
FAU - Yang, Dan
AU  - Yang D
AD  - School of Life Science, Taizhou University, Taizhou 318000, China.
FAU - Zhang, Qi
AU  - Zhang Q
AD  - College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
FAU - Miao, Denian
AU  - Miao D
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences, Shanghai 201106, China.
FAU - He, Xizhong
AU  - He X
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences, Shanghai 201106, China.
FAU - Du, Yanan
AU  - Du Y
AD  - College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
FAU - Zhang, Wanjing
AU  - Zhang W
AD  - College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
FAU - Ni, Jianping
AU  - Ni J
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences, Shanghai 201106, China.
FAU - Zhao, Kai
AU  - Zhao K
AD  - Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of 
      Agricultural Sciences, Shanghai 201106, China.
AD  - Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 
      Shanghai 201106, China.
LA  - eng
GR  - 14140900702, 18391902000 and 17391901800/Science and Technology Commission of 
      Shanghai Municipality/
GR  - [2022] 012/SAAS Program for Excellent Research Team/
PT  - Journal Article
DEP - 20221009
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC9558507
OTO - NOTNLM
OT  - RT-LAMP
OT  - porcine epidemic diarrhea virus
OT  - visual detection
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/15 06:01
PMCR- 2022/10/09
CRDT- 2022/10/14 01:50
PHST- 2022/07/29 00:00 [received]
PHST- 2022/09/29 00:00 [revised]
PHST- 2022/10/06 00:00 [accepted]
PHST- 2022/10/14 01:50 [entrez]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/15 06:01 [medline]
PHST- 2022/10/09 00:00 [pmc-release]
AID - ani12192712 [pii]
AID - animals-12-02712 [pii]
AID - 10.3390/ani12192712 [doi]
PST - epublish
SO  - Animals (Basel). 2022 Oct 9;12(19):2712. doi: 10.3390/ani12192712.