PMID- 36230894
OWN - NLM
STAT- MEDLINE
DCOM- 20221017
LR  - 20221204
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 11
IP  - 19
DP  - 2022 Sep 20
TI  - Circulating Microparticles Are Differentially Increased in Lowlanders and 
      Highlanders with High Altitude Induced Pulmonary Hypertension during the Cold 
      Season.
LID - 10.3390/cells11192932 [doi]
LID - 2932
AB  - The role of microparticles (MPs) and cold in high altitude pulmonary hypertension 
      (HAPH) remains unexplored. We investigated the impact of long-term cold exposure 
      on the pulmonary circulation in lowlanders and high-altitude natives and the role 
      of MPs. Pulmonary hemodynamics were evaluated using Doppler echocardiography at 
      the end of the colder and warmer seasons. We further examined the miRNA content 
      of MPs isolated from the study participants and studied their effects on human 
      pulmonary artery smooth muscle (hPASMCs) and endothelial cells (hPAECs). 
      Long-term exposure to cold environment was associated with an enhanced pulmonary 
      artery pressure in highlanders. Plasma levels of CD62E-positive and CD68-positive 
      MPs increased in response to cold in lowlanders and HAPH highlanders. The 
      miRNA-210 expression contained in MPs differentially changed in response to cold 
      in lowlanders and highlanders. MPs isolated from lowlanders and highlanders 
      increased proliferation and reduced apoptosis of hPASMCs. Further, MPs isolated 
      from warm-exposed HAPH highlanders and cold-exposed highlanders exerted the most 
      pronounced effects on VEGF expression in hPAECs. We demonstrated that prolonged 
      exposure to cold is associated with elevated pulmonary artery pressures, which 
      are most pronounced in high-altitude residents. Further, the numbers of 
      circulating MPs are differentially increased in lowlanders and HAPH highlanders 
      during the colder season.
FAU - Sydykov, Akylbek
AU  - Sydykov A
AUID- ORCID: 0000-0002-8122-7033
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
FAU - Petrovic, Aleksandar
AU  - Petrovic A
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Maripov, Abdirashit M
AU  - Maripov AM
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Gredic, Marija
AU  - Gredic M
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Bermes, Daniel Gerd
AU  - Bermes DG
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Kushubakova, Nadira
AU  - Kushubakova N
AUID- ORCID: 0000-0001-6874-7125
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Muratali Uulu, Kubatbek
AU  - Muratali Uulu K
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Pilz, Christina
AU  - Pilz C
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Cholponbaeva, Meerim
AU  - Cholponbaeva M
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Duishobaev, Melis
AU  - Duishobaev M
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Satybaldyev, Samatbek
AU  - Satybaldyev S
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Satieva, Nurgul
AU  - Satieva N
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
FAU - Mamazhakypov, Argen
AU  - Mamazhakypov A
AUID- ORCID: 0000-0002-2140-4819
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Sartmyrzaeva, Meerim
AU  - Sartmyrzaeva M
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Omurzakova, Nazgul
AU  - Omurzakova N
AUID- ORCID: 0000-0003-3970-9706
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
FAU - Kerimbekova, Zhainagul
AU  - Kerimbekova Z
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Baktybek, Nursultan
AU  - Baktybek N
AUID- ORCID: 0000-0002-0716-9146
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Kulchoroeva, Cholpon
AU  - Kulchoroeva C
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
FAU - Pak, Oleg
AU  - Pak O
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Zhao, Lan
AU  - Zhao L
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
AD  - Department of Medicine, Imperial College London, London SW7 2AZ, UK.
FAU - Weissmann, Norbert
AU  - Weissmann N
AUID- ORCID: 0000-0003-2675-3871
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Avdeev, Sergey
AU  - Avdeev S
AUID- ORCID: 0000-0002-5999-2150
AD  - Department of Pulmonology, Sechenov First Moscow State Medical University 
      (Sechenov University), 119991 Moscow, Russia.
FAU - Maslov, Leonid N
AU  - Maslov LN
AUID- ORCID: 0000-0002-6020-1598
AD  - Laboratory of Experimental Cardiology, Cardiology Research Institute, Tomsk 
      National Research Medical Center, Russian Academy of Science, 634012 Tomsk, 
      Russia.
FAU - Ghofrani, Hossein Ardeschir
AU  - Ghofrani HA
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Schermuly, Ralph Theo
AU  - Schermuly RT
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
FAU - Sarybaev, Akpay S
AU  - Sarybaev AS
AD  - Department of Mountain and Sleep Medicine and Pulmonary Hypertension, National 
      Center of Cardiology and Internal Medicine, Bishkek 720040, Kyrgyzstan.
AD  - Kyrgyz-Indian Mountain Biomedical Research Center, Bishkek 720040, Kyrgyzstan.
FAU - Kosanovic, Djuro
AU  - Kosanovic D
AD  - Department of Internal Medicine, Member of the German Center for Lung Research 
      (DZL), Justus Liebig University of Giessen, 35390 Giessen, Germany.
AD  - Department of Pulmonology, Sechenov First Moscow State Medical University 
      (Sechenov University), 119991 Moscow, Russia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220920
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (MicroRNAs)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - Pulmonary edema of mountaineers
SB  - IM
MH  - Altitude
MH  - Altitude Sickness
MH  - Endothelial Cells
MH  - Humans
MH  - *Hypertension, Pulmonary
MH  - *MicroRNAs
MH  - Seasons
MH  - Vascular Endothelial Growth Factor A
PMC - PMC9563667
OTO - NOTNLM
OT  - chronic cold exposure
OT  - echocardiography
OT  - high altitude
OT  - microparticles
OT  - pulmonary hypertension
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/18 06:00
PMCR- 2022/09/20
CRDT- 2022/10/14 01:53
PHST- 2022/08/18 00:00 [received]
PHST- 2022/09/12 00:00 [revised]
PHST- 2022/09/15 00:00 [accepted]
PHST- 2022/10/14 01:53 [entrez]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/18 06:00 [medline]
PHST- 2022/09/20 00:00 [pmc-release]
AID - cells11192932 [pii]
AID - cells-11-02932 [pii]
AID - 10.3390/cells11192932 [doi]
PST - epublish
SO  - Cells. 2022 Sep 20;11(19):2932. doi: 10.3390/cells11192932.