PMID- 36230915
OWN - NLM
STAT- MEDLINE
DCOM- 20221017
LR  - 20221204
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 11
IP  - 19
DP  - 2022 Sep 21
TI  - Cartilaginous Metabolomics Reveals the Biochemical-Niche Fate Control of Bone 
      Marrow-Derived Stem Cells.
LID - 10.3390/cells11192951 [doi]
LID - 2951
AB  - Joint disorders have become a global health issue with the growth of the aging 
      population. Screening small active molecules targeting chondrogenic 
      differentiation of bone marrow-derived stem cells (BMSCs) is of urgency. In this 
      study, microfracture was employed to create a regenerative niche in rabbits (n = 
      9). Cartilage samples were collected four weeks post-surgery. 
      Microfracture-caused morphological (n = 3) and metabolic (n = 6) changes were 
      detected. Non-targeted metabolomic analysis revealed that there were 96 
      differentially expressed metabolites (DEMs) enriched in 70 pathways involved in 
      anti-inflammation, lipid metabolism, signaling transduction, etc. Among the 
      metabolites, docosapentaenoic acid 22n-3 (DPA) and ursodeoxycholic acid (UDCA) 
      functionally facilitated cartilage defect healing, i.e., increasing the vitality 
      and adaptation of the BMSCs, chondrogenic differentiation, and chondrocyte 
      functionality. Our findings firstly reveal the differences in metabolomic 
      activities between the normal and regenerated cartilages and provide a list of 
      endogenous biomolecules potentially involved in the biochemical-niche fate 
      control for chondrogenic differentiation of BMSCs. Ultimately, the biomolecules 
      may serve as anti-aging supplements for chondrocyte renewal or as drug candidates 
      for cartilage regenerative medicine.
FAU - Peng, Haining
AU  - Peng H
AUID- ORCID: 0000-0001-5136-3790
AD  - Department of Sports Medicine, The Affiliated Hospital of Qingdao University, 
      Qingdao 266000, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Sports Medicine, The Affiliated Hospital of Qingdao University, 
      Qingdao 266000, China.
AD  - Institute of Sports Medicine and Rehabilitation, Qingdao University, Qingdao 
      266000, China.
AD  - Shandong Institute of Traumatic Orthopedics, Medical Research Center, The 
      Affiliated Hospital of Qingdao University, Qingdao 266590, China.
FAU - Ren, Zhongkai
AU  - Ren Z
AD  - Department of Sports Medicine, The Affiliated Hospital of Qingdao University, 
      Qingdao 266000, China.
FAU - Wei, Ziran
AU  - Wei Z
AD  - Department of Sports Medicine, The Affiliated Hospital of Qingdao University, 
      Qingdao 266000, China.
FAU - Chen, Renjie
AU  - Chen R
AD  - Department of Sports Medicine, The Affiliated Hospital of Qingdao University, 
      Qingdao 266000, China.
FAU - Zhang, Yingze
AU  - Zhang Y
AD  - Department of Orthopedics, The Third Hospital of Hebei Medical University, 
      Shijiazhuang 050051, China.
AD  - Department of Orthopedics, The Affiliated Hospital of Qingdao University, Qingdao 
      266000, China.
FAU - Huang, Xiaohong
AU  - Huang X
AUID- ORCID: 0000-0003-3617-8911
AD  - Shandong Institute of Traumatic Orthopedics, Medical Research Center, The 
      Affiliated Hospital of Qingdao University, Qingdao 266590, China.
FAU - Yu, Tengbo
AU  - Yu T
AD  - Department of Sports Medicine, The Affiliated Hospital of Qingdao University, 
      Qingdao 266000, China.
AD  - Institute of Sports Medicine and Rehabilitation, Qingdao University, Qingdao 
      266000, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220921
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 724L30Y2QR (Ursodeoxycholic Acid)
SB  - IM
MH  - Animals
MH  - Bone Marrow
MH  - Cartilage/physiology
MH  - *Fractures, Stress/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Rabbits
MH  - Ursodeoxycholic Acid
PMC - PMC9562901
OTO - NOTNLM
OT  - bone marrow-derived stem cells
OT  - cartilage
OT  - chondrogenic differentiation
OT  - endogenous biomolecules
OT  - metabolomics
OT  - regeneration
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/18 06:00
PMCR- 2022/09/21
CRDT- 2022/10/14 01:53
PHST- 2022/07/22 00:00 [received]
PHST- 2022/08/14 00:00 [revised]
PHST- 2022/09/14 00:00 [accepted]
PHST- 2022/10/14 01:53 [entrez]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/18 06:00 [medline]
PHST- 2022/09/21 00:00 [pmc-release]
AID - cells11192951 [pii]
AID - cells-11-02951 [pii]
AID - 10.3390/cells11192951 [doi]
PST - epublish
SO  - Cells. 2022 Sep 21;11(19):2951. doi: 10.3390/cells11192951.