PMID- 36231009
OWN - NLM
STAT- MEDLINE
DCOM- 20221017
LR  - 20221130
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 11
IP  - 19
DP  - 2022 Sep 29
TI  - Nestin Modulates Airway Smooth Muscle Cell Migration by Affecting Spatial 
      Rearrangement of Vimentin Network and Focal Adhesion Assembly.
LID - 10.3390/cells11193047 [doi]
LID - 3047
AB  - Airway smooth muscle cell migration plays a role in the progression of airway 
      remodeling, a hallmark of allergic asthma. However, the mechanisms that regulate 
      cell migration are not yet entirely understood. Nestin is a class VI intermediate 
      filament protein that is involved in the proliferation/regeneration of neurons, 
      cancer cells, and skeletal muscle. Its role in cell migration is not fully 
      understood. Here, nestin knockdown (KD) inhibited the migration of human airway 
      smooth muscle cells. Using confocal microscopy and the Imaris software, we found 
      that nestin KD attenuated focal adhesion sizes during cell spreading. Moreover, 
      polo-like kinase 1 (Plk1) and vimentin phosphorylation at Ser-56 have been 
      previously shown to affect focal adhesion assembly. Here, nestin KD reduced Plk1 
      phosphorylation at Thr-210 (an indication of Plk1 activation), vimentin 
      phosphorylation at Ser-56, the contacts of vimentin filaments to paxillin, and 
      the morphology of focal adhesions. Moreover, the expression of vimentin 
      phosphorylation-mimic mutant S56D (aspartic acid substitution at Ser-56) rescued 
      the migration, vimentin reorganization, and focal adhesion size of nestin KD 
      cells. Together, our results suggest that nestin promotes smooth muscle cell 
      migration. Mechanistically, nestin regulates Plk1 phosphorylation, which mediates 
      vimenitn phosphorylation, the connection of vimentin filaments with paxillin, and 
      focal adhesion assembly.
FAU - Wang, Ruping
AU  - Wang R
AD  - Department of Molecular and Cellular Physiology, Albany Medical College, Albany, 
      NY 12208, USA.
FAU - Khan, Sakeeb
AU  - Khan S
AUID- ORCID: 0000-0003-4324-3910
AD  - Department of Molecular and Cellular Physiology, Albany Medical College, Albany, 
      NY 12208, USA.
FAU - Liao, Guoning
AU  - Liao G
AD  - Department of Molecular and Cellular Physiology, Albany Medical College, Albany, 
      NY 12208, USA.
FAU - Wu, Yidi
AU  - Wu Y
AD  - Department of Molecular and Cellular Physiology, Albany Medical College, Albany, 
      NY 12208, USA.
FAU - Tang, Dale D
AU  - Tang DD
AUID- ORCID: 0000-0002-7339-9249
AD  - Department of Molecular and Cellular Physiology, Albany Medical College, Albany, 
      NY 12208, USA.
LA  - eng
GR  - R01 HL110951/HL/NHLBI NIH HHS/United States
GR  - R01 HL145392/HL/NHLBI NIH HHS/United States
GR  - R01 HL130304/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220929
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Nestin)
RN  - 0 (Paxillin)
RN  - 0 (Vimentin)
RN  - 30KYC7MIAI (Aspartic Acid)
SB  - IM
MH  - Aspartic Acid
MH  - Cell Movement/physiology
MH  - *Focal Adhesions/metabolism
MH  - Humans
MH  - *Intermediate Filaments/metabolism
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Nestin/genetics/metabolism
MH  - Paxillin/metabolism
MH  - Vimentin/metabolism
PMC - PMC9562664
OTO - NOTNLM
OT  - intermediate filament protein
OT  - migration
OT  - nestin
OT  - smooth muscle
COIS- The authors declare that they have no conflict of interest with the contents of 
      this article.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/18 06:00
PMCR- 2022/09/29
CRDT- 2022/10/14 01:54
PHST- 2022/09/14 00:00 [received]
PHST- 2022/09/26 00:00 [revised]
PHST- 2022/09/27 00:00 [accepted]
PHST- 2022/10/14 01:54 [entrez]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/18 06:00 [medline]
PHST- 2022/09/29 00:00 [pmc-release]
AID - cells11193047 [pii]
AID - cells-11-03047 [pii]
AID - 10.3390/cells11193047 [doi]
PST - epublish
SO  - Cells. 2022 Sep 29;11(19):3047. doi: 10.3390/cells11193047.