PMID- 36232740
OWN - NLM
STAT- MEDLINE
DCOM- 20221017
LR  - 20221019
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 19
DP  - 2022 Sep 28
TI  - NGF-Induced Upregulation of CGRP in Orofacial Pain Induced by Tooth Movement Is 
      Dependent on Atp6v0a1 and Vesicle Release.
LID - 10.3390/ijms231911440 [doi]
LID - 11440
AB  - The nerve growth factor (NGF) and calcitonin gene-related peptide (CGRP) play a 
      crucial role in the regulation of orofacial pain. It has been demonstrated that 
      CGRP increases orofacial pain induced by NGF. V-type proton ATPase subunit an 
      isoform 1 (Atp6v0a1) is involved in the exocytosis pathway, especially in 
      vesicular transport in neurons. The objective was to examine the role of Atp6v0a1 
      in NGF-induced upregulation of CGRP in orofacial pain induced by experimental 
      tooth movement. Orofacial pain was elicited by ligating closed-coil springs 
      between incisors and molars in Sprague-Dawley rats. Gene and protein expression 
      levels were determined through real-time polymerase chain reaction, 
      immunostaining, and fluorescence in situ hybridization. Lentivirus vectors 
      carrying Atp6v0a1 shRNA were used to knockdown the expression of Atp6v0a1 in TG 
      and SH-SY5Y neurons. The release of vesicles in SH-SY5Y neurons was observed by 
      using fluorescence dye FM1-43, and the release of CGRP was detected by 
      Enzyme-Linked Immunosorbent Assy. Orofacial pain was evaluated through the rat 
      grimace scale. Our results revealed that intraganglionic administration of NGF 
      and Atp6v0a1 shRNA upregulated and downregulated CGRP in trigeminal ganglia (TG) 
      and trigeminal subnucleus caudalis (Vc), respectively, and the orofacial pain was 
      also exacerbated and alleviated, respectively, following administration of NGF 
      and Atp6v0a1 shRNA. Besides, intraganglionic administration of NGF simultaneously 
      caused the downregulation of Atp6v0a1 in TG. Moreover, the release of vesicles 
      and CGRP in SH-SY5Y neurons was interfered by NGF and Atp6v0a1 shRNA. In 
      conclusion, in the orofacial pain induced by experimental tooth movement, NGF 
      induced the upregulation of CGRP in TG and Vc, and this process is dependent on 
      Atp6v0a1 and vesicle release, suggesting that they are involved in the 
      transmission of nociceptive information in orofacial pain.
FAU - Tao, Tianjin
AU  - Tao T
AUID- ORCID: 0000-0003-4944-6591
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Sichuan University, Chengdu 610041, China.
FAU - Liu, Yi
AU  - Liu Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Sichuan University, Chengdu 610041, China.
FAU - Zhang, Jingqi
AU  - Zhang J
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Sichuan University, Chengdu 610041, China.
FAU - Lai, Wenli
AU  - Lai W
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Sichuan University, Chengdu 610041, China.
FAU - Long, Hu
AU  - Long H
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Sichuan University, Chengdu 610041, China.
LA  - eng
GR  - 82071147,81571004, and 81500884/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220928
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Immunosorbents)
RN  - 0 (Ngf protein, rat)
RN  - 0 (Protons)
RN  - 0 (RNA, Small Interfering)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Adenosine Triphosphatases/metabolism
MH  - Animals
MH  - *Calcitonin Gene-Related Peptide/genetics/metabolism
MH  - Exocytosis/genetics/physiology
MH  - *Facial Pain/etiology/genetics/metabolism
MH  - Immunosorbents
MH  - In Situ Hybridization, Fluorescence
MH  - *Nerve Growth Factor/genetics/metabolism
MH  - Neuroblastoma
MH  - Neurons/metabolism
MH  - Nociception/physiology
MH  - Protons
MH  - RNA, Small Interfering
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tooth Movement Techniques/methods
MH  - Up-Regulation
MH  - *Vacuolar Proton-Translocating ATPases/genetics/metabolism
PMC - PMC9569904
OTO - NOTNLM
OT  - Atp6v0a1
OT  - CGRP
OT  - NGF
OT  - lentivirus
OT  - orofacial pain
OT  - trigeminal ganglia
OT  - trigeminal subnucleus caudalis
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/18 06:00
PMCR- 2022/09/28
CRDT- 2022/10/14 02:09
PHST- 2022/08/28 00:00 [received]
PHST- 2022/09/15 00:00 [revised]
PHST- 2022/09/15 00:00 [accepted]
PHST- 2022/10/14 02:09 [entrez]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/18 06:00 [medline]
PHST- 2022/09/28 00:00 [pmc-release]
AID - ijms231911440 [pii]
AID - ijms-23-11440 [pii]
AID - 10.3390/ijms231911440 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Sep 28;23(19):11440. doi: 10.3390/ijms231911440.