PMID- 36233243
OWN - NLM
STAT- MEDLINE
DCOM- 20221017
LR  - 20221019
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 19
DP  - 2022 Oct 8
TI  - Diflubenzuron Induces Cardiotoxicity in Zebrafish Embryos.
LID - 10.3390/ijms231911932 [doi]
LID - 11932
AB  - Diflubenzuron is an insecticide that serves as a chitin inhibitor to restrict the 
      growth of many harmful larvae, including mosquito larvae, cotton bollworm and 
      flies. The residue of diflubenzuron is often detected in aquaculture, but its 
      potential toxicity to aquatic organisms is still obscure. In this study, 
      zebrafish embryos (from 6 h to 96 h post-fertilization, hpf) were exposed to 
      different concentrations of diflubenzuron (0, 0.5, 1.5, 2.5, 3.5 and 4.5 mg/L), 
      and the morphologic changes, mortality rate, hatchability rate and average heart 
      rate were calculated. Diflubenzuron exposure increased the distance between the 
      venous sinus and bulbar artery (SV-BA), inhibited proliferation of myocardial 
      cells and damaged vascular development. In addition, diflubenzuron exposure also 
      induced contents of reactive oxygen species (ROS) and malondialdehyde (MDA) and 
      inhibited the activity of antioxidants, including SOD (superoxide dismutase) and 
      CAT (catalase). Moreover, acridine orange (AO) staining showed that diflubenzuron 
      exposure increased the apoptotic cells in the heart. Q-PCR also indicated that 
      diflubenzuron exposure promoted the expression of apoptosis-related genes (bax, 
      bcl2, p53, caspase3 and caspase9). However, the expression of some heart-related 
      genes were inhibited. The oxidative stress-induced apoptosis damaged the cardiac 
      development of zebrafish embryos. Therefore, diflubenzuron exposure induced 
      severe cardiotoxicity in zebrafish embryos. The results contribute to a more 
      comprehensive understanding of the safety use of diflubenzuron.
FAU - Han, Xue
AU  - Han X
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Xu, Xiaowen
AU  - Xu X
AUID- ORCID: 0000-0002-8513-3456
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang 330047, China.
FAU - Yu, Tingting
AU  - Yu T
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Li, Meifeng
AU  - Li M
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Liu, Yulong
AU  - Liu Y
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Lai, Jingli
AU  - Lai J
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Mao, Huiling
AU  - Mao H
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Hu, Chengyu
AU  - Hu C
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
FAU - Wang, Shanghong
AU  - Wang S
AD  - Department of Bioscience, School of Life Science, Nanchang University, Nanchang 
      330031, China.
LA  - eng
GR  - 31960735, 32160871 and 32160872/National Natural Science Foundation of China/
GR  - JXARS-06/Jiangxi Agriculture Research System/
GR  - 2017ZX07301002-05/National Water Pollution Control and Treatment Science and 
      Technology Major Project of China/
GR  - 2019M662279/China Postdoctoral Science Foundation/
GR  - 2019KY43, 2020RC22/Jiangxi Postdoctoral Science Foundation/
PT  - Journal Article
DEP - 20221008
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antioxidants)
RN  - 0 (Insecticides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 1398-61-4 (Chitin)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - F30N4O6XVV (Acridine Orange)
RN  - J76U6ZSI8D (Diflubenzuron)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Cardiotoxicity/metabolism
MH  - Catalase/metabolism
MH  - Chitin/metabolism
MH  - *Diflubenzuron
MH  - Embryo, Nonmammalian/metabolism
MH  - *Insecticides/metabolism
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/genetics
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Tumor Suppressor Protein p53/metabolism
MH  - *Water Pollutants, Chemical/toxicity
MH  - Zebrafish/genetics
MH  - bcl-2-Associated X Protein/metabolism
PMC - PMC9570284
OTO - NOTNLM
OT  - apoptosis
OT  - cardiotoxicity
OT  - diflubenzuron
OT  - oxidative stress
OT  - zebrafish embryo
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/18 06:00
PMCR- 2022/10/08
CRDT- 2022/10/14 02:13
PHST- 2022/08/29 00:00 [received]
PHST- 2022/09/20 00:00 [revised]
PHST- 2022/09/29 00:00 [accepted]
PHST- 2022/10/14 02:13 [entrez]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/18 06:00 [medline]
PHST- 2022/10/08 00:00 [pmc-release]
AID - ijms231911932 [pii]
AID - ijms-23-11932 [pii]
AID - 10.3390/ijms231911932 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Oct 8;23(19):11932. doi: 10.3390/ijms231911932.