PMID- 36233871 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221019 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 15 IP - 19 DP - 2022 Sep 21 TI - Incorporation of Mixing Microbial Induced Calcite Precipitation (MICP) with Pretreatment Procedure for Road Soil Subgrade Stabilization. LID - 10.3390/ma15196529 [doi] LID - 6529 AB - Microbial induced carbonate precipitation (MICP) provides an alternative method to stabilize the soil. To further improve the reinforcement effect, this study aims to propose a strategy by incorporating the mixing MICP method with pretreatment procedure. A series of laboratory tests were performed to investigate the preparation parameters (including the moisture content and dry density of the soil, the concentration of urea and CaCl(2) in cementation solution), the engineering properties, the CaCO(3) distribution as well as the mineralogical and micro structural characteristics of pretreatment-mixing MICP reinforced soil (PMMRS). Based on the orthogonal experiment results, the optimum preparation parameters for PMMRS were determined. The UCS of PMMRS was more strongly dependent on the moisture content and concentration of CaCl(2) than the concentration ratio of CaCl(2) to urea. Moreover, it was testified that incorporation of pretreatment procedure improved the stabilization effect of traditional mixing MICP method on the clayed sand (CLS). The UCS of PMMRS specimen was increased by 198% and 78% for the pure CLS and the simple mixing MICP reinforced soil, respectively. Furthermore, the CaCO(3) products generated consisted of the aragonite, calcite and vaterite, which distributed unevenly inside the specimen no matter the lateral or vertical direction. The reason for the uneven distribution might be that oxygen content varied with the regions in different directions, and hence affected the mineralization reaction. In addition, the mineralization reaction would affect the pore structure of the soil, which was highly related to the stabilization effect of MICP reinforced soil. FAU - Hu, Xiaodi AU - Hu X AD - School of Civil Engineering and Architecture, Wuhan Institute of Technology, Wuhan 430205, China. AD - Hubei Provincial Engineering Research Center for Green Civil Engineering Materials and Structures, Wuhan 430073, China. FAU - Fu, Xiongzheng AU - Fu X AUID- ORCID: 0000-0002-0978-0360 AD - School of Civil Engineering and Architecture, Wuhan Institute of Technology, Wuhan 430205, China. FAU - Pan, Pan AU - Pan P AD - School of Civil Engineering and Architecture, Wuhan Institute of Technology, Wuhan 430205, China. AD - Hubei Provincial Engineering Research Center for Green Civil Engineering Materials and Structures, Wuhan 430073, China. FAU - Lin, Lirong AU - Lin L AD - School of Civil Engineering and Architecture, Wuhan Institute of Technology, Wuhan 430205, China. AD - Hubei Provincial Academy of Building Research and Design Co., Ltd., Wuhan 430071, China. FAU - Sun, Yihan AU - Sun Y AD - Zhejiang Academy of Transportation Sciences, Hangzhou 310023, China. LA - eng GR - No. 2019CFB330/the Nature Science Foundation of Hubei Province/ GR - No. LGG21E080002/Zhejiang Provincial Natural Science Foundation/ GR - No. 2022-11-1-2/Technology Project of Department of Transportation of Hubei Province/ PT - Journal Article DEP - 20220921 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC9573274 OTO - NOTNLM OT - microbial induced calcite precipitation (MICP) OT - pretreatment-mixing MICP method OT - subgrade soil stabilization COIS- The authors declare no conflict of interest. EDAT- 2022/10/15 06:00 MHDA- 2022/10/15 06:01 PMCR- 2022/09/21 CRDT- 2022/10/14 02:18 PHST- 2022/07/29 00:00 [received] PHST- 2022/09/13 00:00 [revised] PHST- 2022/09/15 00:00 [accepted] PHST- 2022/10/14 02:18 [entrez] PHST- 2022/10/15 06:00 [pubmed] PHST- 2022/10/15 06:01 [medline] PHST- 2022/09/21 00:00 [pmc-release] AID - ma15196529 [pii] AID - materials-15-06529 [pii] AID - 10.3390/ma15196529 [doi] PST - epublish SO - Materials (Basel). 2022 Sep 21;15(19):6529. doi: 10.3390/ma15196529.