PMID- 36237191 OWN - NLM STAT- MEDLINE DCOM- 20221017 LR - 20221017 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 13 DP - 2022 TI - Decreased Urine N6-methyladenosine level is closely associated with the presence of diabetic nephropathy in type 2 diabetes mellitus. PG - 986419 LID - 10.3389/fendo.2022.986419 [doi] LID - 986419 AB - BACKGROUND: To investigate the dynamic changes of urine N6-methyladenosine (m6A) levels in patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN) and evaluate the clinical significance. METHODS: First, the levels of urine m6A were examined and compared among 62 patients with T2DM, 70 patients with DN, and 52 age- and gender-matched normal glucose tolerant subjects (NGT) by using a MethyIFIashTM Urine m6A Quantification Kit. Subsequently, we compared the concentrations of urine m6A between different stages of DN. Moreover, statistical analysis was performed to evaluate the association of urine m6A with DN. RESULTS: The levels of m6A were significantly decreased in patients with DN [(16.10 +/- 6.48) ng/ml], compared with NGT [(23.12 +/- 7.52) ng/ml, P < 0.0001] and patients with T2DM [(20.39 +/- 7.16) ng/ml, P < 0.0001]. Moreover, the concentrations of urine m6A were obviously reduced with the deterioration of DN. Pearson rank correlation and regression analyses revealed that m6A was significantly associated with DN (P < 0.05). The areas under the receiver operator characteristics curve (AUC) were 0.783 (95% CI, 0.699 - 0.867, P < 0.0001) for the DN and NGT groups, and 0.737 (95% CI, 0.639 - 0.835, P < 0.0001) for the macroalbuminuria and normoalbuminuria groups, and the optimal cutoff value for m6A to distinguish the DN from NGT and the macroalbuminuria from normoalbuminuria cases was 0.4687 (diagnostic sensitivity, 71%; diagnostic specificity, 76%) and 0.4494 (diagnostic sensitivity, 79%; diagnostic specificity, 66%), respectively. CONCLUSIONS: The levels of urine m6A are significantly decreased in patients with DN and change with the deterioration of DN, which could serve as a prospective biomarker for the diagnosis of DN. CI - Copyright (c) 2022 Wan, Hua, Xing, Cheng, Zhou, Sun, Yao, Meng, Cheng, Wu, Zhai, Zhang, Kong and Lv. FAU - Wan, Shu-Jun AU - Wan SJ AD - Central Laboratory, The first affiliated hospital of Wannan Medical College, Wuhu, China. AD - Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes (Wannan Medical College), Wuhu, China. AD - Anhui Province Clinical Research Center for Critical Respiratory Medicine, Wuhu, China. FAU - Hua, Qiang AU - Hua Q AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Xing, Yu-Jie AU - Xing YJ AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Cheng, Yi AU - Cheng Y AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Zhou, Si-Min AU - Zhou SM AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Sun, Yue AU - Sun Y AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Yao, Xin-Ming AU - Yao XM AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Meng, Xiang-Jian AU - Meng XJ AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Cheng, Jin-Han AU - Cheng JH AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Wu, Han AU - Wu H AD - Anhui Province Clinical Research Center for Critical Respiratory Medicine, Wuhu, China. AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Zhai, Qing AU - Zhai Q AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Zhang, Yan AU - Zhang Y AD - Central Laboratory, The first affiliated hospital of Wannan Medical College, Wuhu, China. FAU - Kong, Xiang AU - Kong X AD - Central Laboratory, The first affiliated hospital of Wannan Medical College, Wuhu, China. AD - Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes (Wannan Medical College), Wuhu, China. AD - Anhui Province Clinical Research Center for Critical Respiratory Medicine, Wuhu, China. AD - Department of Endocrinology, The first affiliated hospital of Wannan Medical College, Yijishan Hospital, Wuhu, China. FAU - Lv, Kun AU - Lv K AD - Central Laboratory, The first affiliated hospital of Wannan Medical College, Wuhu, China. AD - Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes (Wannan Medical College), Wuhu, China. AD - Anhui Province Clinical Research Center for Critical Respiratory Medicine, Wuhu, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220927 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 0 (Biomarkers) RN - CLE6G00625 (N-methyladenosine) RN - IY9XDZ35W2 (Glucose) RN - K72T3FS567 (Adenosine) SB - IM MH - Adenosine/analogs & derivatives MH - Biomarkers/urine MH - *Diabetes Mellitus, Type 2/complications/diagnosis MH - *Diabetic Nephropathies/diagnosis/etiology MH - Glucose MH - Humans PMC - PMC9553099 OTO - NOTNLM OT - N6-methyladenosine OT - biomarkers OT - diabetic nephropathy OT - type 2 diabetes mellitus OT - urine COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/10/15 06:00 MHDA- 2022/10/18 06:00 PMCR- 2022/01/01 CRDT- 2022/10/14 02:45 PHST- 2022/07/05 00:00 [received] PHST- 2022/08/22 00:00 [accepted] PHST- 2022/10/14 02:45 [entrez] PHST- 2022/10/15 06:00 [pubmed] PHST- 2022/10/18 06:00 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fendo.2022.986419 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2022 Sep 27;13:986419. doi: 10.3389/fendo.2022.986419. eCollection 2022.