PMID- 36239140
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20230202
LR  - 20230415
IS  - 1439-7641 (Electronic)
IS  - 1439-4235 (Print)
IS  - 1439-4235 (Linking)
VI  - 24
IP  - 3
DP  - 2023 Feb 1
TI  - Far Red-Shifted CdTe Quantum Dots for Multicolour Stimulated Emission Depletion 
      Nanoscopy.
PG  - e202200698
LID - 10.1002/cphc.202200698 [doi]
LID - e202200698
AB  - Stimulated emission depletion (STED) nanoscopy is a widely used nanoscopy 
      technique. Two-colour STED imaging in fixed and living cells is standardised 
      today utilising both fluorescent dyes and fluorescent proteins. Solutions to 
      image additional colours have been demonstrated using spectral unmixing, 
      photobleaching steps, or long-Stokes-shift dyes. However, these approaches often 
      compromise speed, spatial resolution, and image quality, and increase complexity. 
      Here, we present multicolour STED nanoscopy with far red-shifted semiconductor 
      CdTe quantum dots (QDs). STED imaging of the QDs is optimized to minimize 
      blinking effects and maximize the number of detected photons. The far-red and 
      compact emission spectra of the investigated QDs free spectral space for the 
      simultaneous use of fluorescent dyes, enabling straightforward three-colour STED 
      imaging with a single depletion beam. We use our method to study the 
      internalization of QDs in cells, opening up the way for future super-resolution 
      studies of particle uptake and internalization.
CI  - (c) 2022 The Authors. ChemPhysChem published by Wiley-VCH GmbH.
FAU - Alvelid, Jonatan
AU  - Alvelid J
AUID- ORCID: 0000-0002-3554-9322
AD  - Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, 
      114 28, Stockholm, Sweden.
FAU - Bucci, Andrea
AU  - Bucci A
AD  - Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, 
      114 28, Stockholm, Sweden.
FAU - Testa, Ilaria
AU  - Testa I
AUID- ORCID: 0000-0003-4005-4997
AD  - Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, 
      114 28, Stockholm, Sweden.
LA  - eng
GR  - 638314/ERC Starting Grant/
GR  - Horizon 2020/
GR  - ERC_/European Research Council/International
PT  - Journal Article
DEP - 20221109
PL  - Germany
TA  - Chemphyschem
JT  - Chemphyschem : a European journal of chemical physics and physical chemistry
JID - 100954211
SB  - IM
PMC - PMC10098508
OTO - NOTNLM
OT  - biophysics
OT  - microscopy
OT  - multicolour
OT  - quantum dots
OT  - stimulated emission depletion
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/15 06:00
MHDA- 2022/10/15 06:01
PMCR- 2023/04/13
CRDT- 2022/10/14 05:02
PHST- 2022/10/07 00:00 [revised]
PHST- 2022/09/16 00:00 [received]
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/10/15 06:01 [medline]
PHST- 2022/10/14 05:02 [entrez]
PHST- 2023/04/13 00:00 [pmc-release]
AID - CPHC202200698 [pii]
AID - 10.1002/cphc.202200698 [doi]
PST - ppublish
SO  - Chemphyschem. 2023 Feb 1;24(3):e202200698. doi: 10.1002/cphc.202200698. Epub 2022 
      Nov 9.