PMID- 36239355
OWN - NLM
STAT- MEDLINE
DCOM- 20221228
LR  - 20230211
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Print)
IS  - 1672-9145 (Linking)
VI  - 54
IP  - 10
DP  - 2022 Oct 25
TI  - Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in 
      inflammatory processes.
PG  - 1507-1517
LID - 10.3724/abbs.2022143 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) plays a crucial role in various 
      inflammatory diseases. To reveal the impact of MCP-1 during diseases and to 
      develop anti-inflammatory agents, we establish a transgenic mouse line. The 
      firefly luciferase gene is incorporated into the mouse genome and driven by the 
      endogenous MCP-1 promoter. A bioluminescence photographing system is applied to 
      monitor luciferase levels in live mice during inflammation, including 
      lipopolysaccharide-induced sepsis, concanavalin A-induced T cell-dependent liver 
      injury, CCl (4)-induced acute hepatitis, and liver fibrosis. The results 
      demonstrate that the luciferase signal induced in inflammatory processes is 
      correlated with endogenous MCP-1 expression in mice. Furthermore, the expressions 
      of MCP-1 and the luciferase gene are dramatically inhibited by administration of 
      the anti-inflammatory drug dexamethasone in a septicemia model. Our results 
      suggest that the transgenic MCP-1-Luc mouse is a useful model to study MCP-1 
      expression in inflammation and disease and to evaluate the efficiency of 
      anti-inflammatory drugs in vivo.
FAU - Shao, Fangyang
AU  - Shao F
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
AD  - Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
AD  - College of Life Sciences, University of Chinese Academy of Sciences, Beijing 
      100049, China.
FAU - Ci, Lei
AU  - Ci L
AD  - Shanghai Engineering Research Center for Model Organisms, SMOC, Shanghai 201203, 
      China.
FAU - Shi, Jiahao
AU  - Shi J
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Fang, Fei
AU  - Fang F
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Yan, Bowen
AU  - Yan B
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Liu, Xijun
AU  - Liu X
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Yao, Xiangyu
AU  - Yao X
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Zhang, Mengjie
AU  - Zhang M
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Yang, Hua
AU  - Yang H
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
FAU - Wang, Zhugang
AU  - Wang Z
AD  - Shanghai Engineering Research Center for Model Organisms, SMOC, Shanghai 201203, 
      China.
FAU - Fei, Jian
AU  - Fei J
AD  - School of Life Sciences and Technology, Tongji University, Shanghai 200092, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Chemokine CCL2)
RN  - 0 (Anti-Inflammatory Agents)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Chemokine CCL2/genetics
MH  - *Anti-Inflammatory Agents/pharmacology
MH  - Mice, Transgenic
MH  - Inflammation/genetics
MH  - Luciferases/genetics
PMC - PMC9828394
OTO - NOTNLM
OT  - MCP-1
OT  - bioluminescence imaging
OT  - luciferase
OT  - mouse
OT  - promoter
EDAT- 2022/10/15 06:00
MHDA- 2022/12/29 06:00
PMCR- 2022/10/13
CRDT- 2022/10/14 07:42
PHST- 2022/10/15 06:00 [pubmed]
PHST- 2022/12/29 06:00 [medline]
PHST- 2022/10/14 07:42 [entrez]
PHST- 2022/10/13 00:00 [pmc-release]
AID - 10.3724/abbs.2022143 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2022 Oct 25;54(10):1507-1517. doi: 
      10.3724/abbs.2022143.