PMID- 36244481
OWN - NLM
STAT- MEDLINE
DCOM- 20221202
LR  - 20221202
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 858
IP  - Pt 1
DP  - 2023 Feb 1
TI  - Understanding the metabolism of the novel plant antiviral agent dufulin by 
      different positional (14)C labeling in cherry radishes.
PG  - 159396
LID - S0048-9697(22)06495-6 [pii]
LID - 10.1016/j.scitotenv.2022.159396 [doi]
AB  - Dufulin is a new type of plant antiviral agent. However, its metabolism in 
      plants, which is very important for environmental risk assessment, is still 
      unclear. In this study, we used (14)C markers at different positions and 
      high-performance liquid chromatography-quadrupole time-of-flight-mass 
      spectrometry (HPLC-QTOF-MS) to qualitatively and quantitatively analyze dufulin 
      metabolites in cherry radish. By combining ion pairs with unique abundance 
      ratios, we clarified the metabolite structures, inferred the metabolic pathway of 
      dufulin, and clarified the criteria for residues. The extractable residue of 
      dufulin from cherry radish stem and leaf tissues was above 98 % and that in the 
      succulent root was above 87 %. In the stem and leaf tissues and succulent root, 
      dufulin underwent both phase I and phase II metabolism, and four metabolites were 
      produced, including a conjugate of glucose malonate and hydroxylated dufulin, 
      which was confirmed by comparison with a standard. However, the proportions and 
      concentrations of the four metabolites did not meet the residue criteria, so only 
      the dufulin precursor compound was included as a residue. This study provides 
      reliable data for evaluating the impacts of dufulin on the environment and human 
      health and for objectively examining the safety of dufulin.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Zheng, Ruonan
AU  - Zheng R
AD  - Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear 
      Agricultural Sciences of Ministry of Agriculture of the PRC and Zhejiang 
      Province, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      11816023@zju.edu.cn.
FAU - Shao, Siyao
AU  - Shao S
AD  - Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear 
      Agricultural Sciences of Ministry of Agriculture of the PRC and Zhejiang 
      Province, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      siyaoshao@zju.edu.cn.
FAU - Li, Xiaofeng
AU  - Li X
AD  - Institute of Catalysis, Key Laboratory of Applied Chemistry of Zhejiang Province, 
      Zhejiang University, Hangzhou 310058, China. Electronic address: 
      lxf_88@zju.edu.edu.cn.
FAU - Zhang, Weiwei
AU  - Zhang W
AD  - Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear 
      Agricultural Sciences of Ministry of Agriculture of the PRC and Zhejiang 
      Province, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      21916008@zju.edu.cn.
FAU - Zhang, Sufen
AU  - Zhang S
AD  - Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear 
      Agricultural Sciences of Ministry of Agriculture of the PRC and Zhejiang 
      Province, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      sfzhang@zju.edu.cn.
FAU - Yu, Zhiyang
AU  - Yu Z
AD  - Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear 
      Agricultural Sciences of Ministry of Agriculture of the PRC and Zhejiang 
      Province, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      yuzhiyang@zju.edu.cn.
FAU - Ye, Qingfu
AU  - Ye Q
AD  - Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear 
      Agricultural Sciences of Ministry of Agriculture of the PRC and Zhejiang 
      Province, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      qfye@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20221013
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (dufulin)
RN  - 0 (Antiviral Agents)
RN  - 0 (Benzothiazoles)
SB  - IM
MH  - Humans
MH  - *Raphanus
MH  - Antiviral Agents
MH  - Benzothiazoles/analysis
MH  - Chromatography, High Pressure Liquid/methods
OTO - NOTNLM
OT  - Cherry radish
OT  - Diverse (14)C labeling
OT  - HPLC-QTOF-MS
OT  - Metabolites
OT  - Residue
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/10/17 06:00
MHDA- 2022/12/03 06:00
CRDT- 2022/10/16 19:44
PHST- 2022/09/08 00:00 [received]
PHST- 2022/10/03 00:00 [revised]
PHST- 2022/10/08 00:00 [accepted]
PHST- 2022/10/17 06:00 [pubmed]
PHST- 2022/12/03 06:00 [medline]
PHST- 2022/10/16 19:44 [entrez]
AID - S0048-9697(22)06495-6 [pii]
AID - 10.1016/j.scitotenv.2022.159396 [doi]
PST - ppublish
SO  - Sci Total Environ. 2023 Feb 1;858(Pt 1):159396. doi: 
      10.1016/j.scitotenv.2022.159396. Epub 2022 Oct 13.