PMID- 36244649
OWN - NLM
STAT- MEDLINE
DCOM- 20231106
LR  - 20231113
IS  - 2320-2890 (Electronic)
IS  - 2319-4170 (Print)
IS  - 2319-4170 (Linking)
VI  - 46
IP  - 5
DP  - 2023 Oct
TI  - The effects of intermittent hypoxia on hepatic expression of fatty acid 
      translocase CD36 in lean and diet-induced obese mice.
PG  - 100566
LID - S2319-4170(22)00141-X [pii]
LID - 10.1016/j.bj.2022.10.003 [doi]
LID - 100566
AB  - BACKGROUND: Both obstructive sleep apnea (OSA) and non-alcoholic fatty liver 
      disease (NAFLD) are prevalent within obese individuals. We aimed to investigate 
      the effects of intermittent hypoxia (IH), a clinical feature of OSA, on hepatic 
      expression of fatty acid translocase (CD36) in relation to liver injury in lean 
      and diet-induced obese mice. METHODS: Four-week-old male C57BL/6J mice were 
      randomized to standard diet (SD) or high fat (HF) diet groups. At 13-week-old, 
      all mice were exposed to either air or IH (IH30; thirty hypoxic episodes per 
      hour) for four weeks. We assessed liver injury through lipid profile, oxidative 
      and inflammatory stress, histological scoring and hepatic CD36 expression. 
      RESULTS: In lean mice, IH elevated serum and hepatic triglyceride and free fatty 
      acid (FFA) levels, in line with upregulation of hepatic CD36 expression and 
      myeloperoxidase (MPO)-positive cells in support of inflammatory infiltrates along 
      with increase in serum malondialdehyde (MDA), C-X-C motif chemokine ligand 
      1(CXCL-1) and monocyte chemoattractant protein-1 (MCP-1). In diet-induced obese 
      mice, an increase in hepatic alanine transaminase (ALT) activity, serum and 
      hepatic levels of lipid parameters and inflammatory markers, serum MDA level, 
      hepatic expressions of CD36 and alpha-smooth muscle actin (alpha-SMA), and MPO-positive 
      cells was observed. IH potentiated hepatic ALT activity, serum CXCL-1 and hepatic 
      interleukin-6 (IL-6), in line with inflammatory infiltrates, but paradoxically, 
      reduced hepatic FFA level and hepatic CD36 expression, compared to obese mice 
      without IH exposure. However, IH further augmented diet-induced liver steatosis 
      and fibrosis as shown by histological scores. CONCLUSION: This study contributes 
      to support that IH featuring OSA may lead to liver injury via differential 
      regulation of hepatic CD36 expression in lean and diet-induced obese mice.
CI  - Copyright (c) 2022 Chang Gung University. Published by Elsevier B.V. All rights 
      reserved.
FAU - Ji, Yang
AU  - Ji Y
AD  - Respiratory Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 
      China.
FAU - Liang, Yingmin
AU  - Liang Y
AD  - Respiratory Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 
      China; Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
FAU - Chu, Pak Hin
AU  - Chu PH
AD  - Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
FAU - Ge, Mengqin
AU  - Ge M
AD  - Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
FAU - Yeung, Sze Chun
AU  - Yeung SC
AD  - Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
FAU - Ip, Mary Sau Man
AU  - Ip MSM
AD  - Respiratory Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 
      China; Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
FAU - Mak, Judith Choi Wo
AU  - Mak JCW
AD  - Respiratory Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 
      China; Department of Medicine, The University of Hong Kong, Hong Kong SAR, China; 
      Department of Pharmacology & Pharmacy, The University of Hong Kong, Hong Kong 
      SAR, China. Electronic address: judithmak@hku.hk.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
DEP - 20221013
PL  - United States
TA  - Biomed J
JT  - Biomedical journal
JID - 101599820
RN  - 0 (Triglycerides)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Male
MH  - Mice
MH  - Animals
MH  - Mice, Obese
MH  - Mice, Inbred C57BL
MH  - *Liver/pathology
MH  - Hypoxia/metabolism/pathology
MH  - Diet, High-Fat/adverse effects
MH  - Triglycerides/metabolism
MH  - Fatty Acids/metabolism
MH  - *Sleep Apnea, Obstructive
PMC - PMC10498409
OTO - NOTNLM
OT  - Inflammation
OT  - Intermittent hypoxia
OT  - Liver injury
OT  - Obstructive sleep apnea
OT  - Oxidative stress
COIS- Conflicts of interest The authors have no Conflicts of interest to declare.
EDAT- 2022/10/17 06:00
MHDA- 2023/11/06 06:41
PMCR- 2022/10/13
CRDT- 2022/10/16 19:47
PHST- 2022/01/21 00:00 [received]
PHST- 2022/09/01 00:00 [revised]
PHST- 2022/10/11 00:00 [accepted]
PHST- 2023/11/06 06:41 [medline]
PHST- 2022/10/17 06:00 [pubmed]
PHST- 2022/10/16 19:47 [entrez]
PHST- 2022/10/13 00:00 [pmc-release]
AID - S2319-4170(22)00141-X [pii]
AID - 100566 [pii]
AID - 10.1016/j.bj.2022.10.003 [doi]
PST - ppublish
SO  - Biomed J. 2023 Oct;46(5):100566. doi: 10.1016/j.bj.2022.10.003. Epub 2022 Oct 13.